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Article: Microbial characteristics of a methanogenic phenol-degrading sludge

TitleMicrobial characteristics of a methanogenic phenol-degrading sludge
Authors
Issue Date2005
PublisherI W A Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwst
Citation
Water Science And Technology, 2005, v. 52 n. 1-2, p. 73-78 How to Cite?
AbstractMicrobial properties of a methanogenic granular phenol-degrading sludge were characterized using the 16S rRNA/DNA-based techniques, including polymerase chain reaction (PCR) amplification, cloning, DNA sequencing, and fluorescence in situ hybridization (FISH). The sludge was sampled from an upflow anaerobic sludge blanket reactor, which removed 98% of phenol (up to 1260 mg/l) in wastewater at 26°C with 12 hours of hydraulic retention. Based on DNA analysis, the Eubacteria in the sludge was composed of 13 operational taxonomy units (OTUs). Two OTUs, one resembling Clostridium and the other remotely resembling Desulfotomaculum, were likely responsible for the conversion of phenol to benzoate, which was further degraded by five Syntrophus-resembling OTUs to acetate and H2/CO2; methanogens lastly converted acetate and H2/CO2 into methane. The role of six remaining OTUs remains unclear. Overall, the sludge was composed of 26 ± 6% Eubacteria and 74 ± 9% methanogens, of which 54 ± 6% were acetotrophic Methanosaetaceae, 14 ± 3% and 3 ± 2% were hydrogenotrophic Methanomicrobiales and Methanobacteriaceae, respectively. © IWA Publishing 2005.
Persistent Identifierhttp://hdl.handle.net/10722/71463
ISSN
2015 Impact Factor: 1.064
2015 SCImago Journal Rankings: 0.469
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorKe, SZen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorFang, HPen_HK
dc.date.accessioned2010-09-06T06:32:13Z-
dc.date.available2010-09-06T06:32:13Z-
dc.date.issued2005en_HK
dc.identifier.citationWater Science And Technology, 2005, v. 52 n. 1-2, p. 73-78en_HK
dc.identifier.issn0273-1223en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71463-
dc.description.abstractMicrobial properties of a methanogenic granular phenol-degrading sludge were characterized using the 16S rRNA/DNA-based techniques, including polymerase chain reaction (PCR) amplification, cloning, DNA sequencing, and fluorescence in situ hybridization (FISH). The sludge was sampled from an upflow anaerobic sludge blanket reactor, which removed 98% of phenol (up to 1260 mg/l) in wastewater at 26°C with 12 hours of hydraulic retention. Based on DNA analysis, the Eubacteria in the sludge was composed of 13 operational taxonomy units (OTUs). Two OTUs, one resembling Clostridium and the other remotely resembling Desulfotomaculum, were likely responsible for the conversion of phenol to benzoate, which was further degraded by five Syntrophus-resembling OTUs to acetate and H2/CO2; methanogens lastly converted acetate and H2/CO2 into methane. The role of six remaining OTUs remains unclear. Overall, the sludge was composed of 26 ± 6% Eubacteria and 74 ± 9% methanogens, of which 54 ± 6% were acetotrophic Methanosaetaceae, 14 ± 3% and 3 ± 2% were hydrogenotrophic Methanomicrobiales and Methanobacteriaceae, respectively. © IWA Publishing 2005.en_HK
dc.languageengen_HK
dc.publisherI W A Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwsten_HK
dc.relation.ispartofWater Science and Technologyen_HK
dc.subject.meshBacteria - classification - genetics - isolation & purification - metabolismen_HK
dc.subject.meshBacteria, Anaerobic - classification - genetics - isolation & purification - metabolismen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBiodegradation, Environmentalen_HK
dc.subject.meshBioreactorsen_HK
dc.subject.meshDNA, Archaeal - analysisen_HK
dc.subject.meshDNA, Bacterial - analysisen_HK
dc.subject.meshEuryarchaeota - classification - genetics - isolation & purification - metabolismen_HK
dc.subject.meshIn Situ Hybridization, Fluorescenceen_HK
dc.subject.meshMethane - metabolismen_HK
dc.subject.meshPhenol - metabolismen_HK
dc.subject.meshRNA, Ribosomal, 16S - analysisen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSewage - microbiologyen_HK
dc.titleMicrobial characteristics of a methanogenic phenol-degrading sludgeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0273-1223&volume=52&issue=1-2&spage=73&epage=78&date=2005&atitle=Microbial+characteristics+of+a+methanogenic+phenol-degrading+sludgeen_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.emailFang, HP:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityFang, HP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid16180411-
dc.identifier.scopuseid_2-s2.0-25144521659en_HK
dc.identifier.hkuros104765en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-25144521659&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage73en_HK
dc.identifier.epage78en_HK
dc.identifier.isiWOS:000232146500010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridKe, SZ=7007049344en_HK
dc.identifier.scopusauthoridLiu, Y=35424308000en_HK
dc.identifier.scopusauthoridFang, HP=7402542625en_HK

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