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Article: Microbial community dynamics during start-up of acidogenic anaerobic reactors

TitleMicrobial community dynamics during start-up of acidogenic anaerobic reactors
Authors
Issue Date2002
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/watres
Citation
Water Research, 2002, v. 36 n. 13, p. 3203-3210 How to Cite?
AbstractStart-up of two acidogenic reactors under mesophilic (37°C) and thermophilic (55°C) conditions was carried out with methanogenic granular sludge as an inoculum and dairy wastewater as feed. During these 71 days of the start-up period, microbial community dynamics in these two acidogenic reactors, as monitored by denaturing gradient gel electrophoresis (DGGE) and dot-blot hybridization with group-specific oligonucleotide probes, was correlated to reactor performance. Due to pH drop to 5.5, DGGE community fingerprints for domains Bacteria and Archaea populations showed significant shifts after 13 days of operation, and this change was accompanied with an increase in volatile fatty acid production, a decrease in methane formation, and rapid sludge disintegration. Dot-blot hybridization results further indicated that the decrease in methane production was related to the decrease in Archaea population in particular with methanogens from 34.1% of total 16S-rRNA in the seed sludge to 8% within the first 13 days, and to 2-5% at day 71. Among the methanogens monitored, the class Methanomicrobiales was the most abundant followed up by Methanobacteriales and Methanococcales. Due to an elevated temperature, the microbial community change was more significant and rapid in the thermophilic reactor than in the mesophilic reactor. Significant microbial population changes took place at the first 13 days for both reactors, but a longer period up to 71 days was required to establish a microbial community with a stable metabolic activity. © 2002 Elsevier Science Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/71432
ISSN
2015 Impact Factor: 5.991
2015 SCImago Journal Rankings: 2.772
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, WTen_HK
dc.contributor.authorChan, OCen_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2010-09-06T06:31:56Z-
dc.date.available2010-09-06T06:31:56Z-
dc.date.issued2002en_HK
dc.identifier.citationWater Research, 2002, v. 36 n. 13, p. 3203-3210en_HK
dc.identifier.issn0043-1354en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71432-
dc.description.abstractStart-up of two acidogenic reactors under mesophilic (37°C) and thermophilic (55°C) conditions was carried out with methanogenic granular sludge as an inoculum and dairy wastewater as feed. During these 71 days of the start-up period, microbial community dynamics in these two acidogenic reactors, as monitored by denaturing gradient gel electrophoresis (DGGE) and dot-blot hybridization with group-specific oligonucleotide probes, was correlated to reactor performance. Due to pH drop to 5.5, DGGE community fingerprints for domains Bacteria and Archaea populations showed significant shifts after 13 days of operation, and this change was accompanied with an increase in volatile fatty acid production, a decrease in methane formation, and rapid sludge disintegration. Dot-blot hybridization results further indicated that the decrease in methane production was related to the decrease in Archaea population in particular with methanogens from 34.1% of total 16S-rRNA in the seed sludge to 8% within the first 13 days, and to 2-5% at day 71. Among the methanogens monitored, the class Methanomicrobiales was the most abundant followed up by Methanobacteriales and Methanococcales. Due to an elevated temperature, the microbial community change was more significant and rapid in the thermophilic reactor than in the mesophilic reactor. Significant microbial population changes took place at the first 13 days for both reactors, but a longer period up to 71 days was required to establish a microbial community with a stable metabolic activity. © 2002 Elsevier Science Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/watresen_HK
dc.relation.ispartofWater Researchen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBacteria, Anaerobic - physiologyen_HK
dc.subject.meshBioreactorsen_HK
dc.subject.meshCattleen_HK
dc.subject.meshPopulation Dynamicsen_HK
dc.subject.meshRNA, Ribosomal, 16S - analysisen_HK
dc.subject.meshSewage - microbiologyen_HK
dc.subject.meshTemperatureen_HK
dc.subject.meshWaste Disposal, Fluiden_HK
dc.titleMicrobial community dynamics during start-up of acidogenic anaerobic reactorsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0043-1354&volume=36&issue=13&spage=3203&epage=3210&date=2002&atitle=Microbial+community+dynamics+during+start-up+of+acidogenic+anaerobic+reactorsen_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0043-1354(02)00022-2en_HK
dc.identifier.pmid12188116-
dc.identifier.scopuseid_2-s2.0-0036661667en_HK
dc.identifier.hkuros76011en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036661667&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue13en_HK
dc.identifier.spage3203en_HK
dc.identifier.epage3210en_HK
dc.identifier.isiWOS:000177441400004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLiu, WT=37045931100en_HK
dc.identifier.scopusauthoridChan, OC=7004275779en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK

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