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Conference Paper: Study of microbial community of brewery-treating granular sludge by denaturing gradient gel electrophoresis of 16S rRNA gene

TitleStudy of microbial community of brewery-treating granular sludge by denaturing gradient gel electrophoresis of 16S rRNA gene
Authors
Issue Date2001
PublisherIWA Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwst
Citation
Water Science And Technology, 2001, v. 43 n. 1, p. 77-82 How to Cite?
AbstractThe microbial community structure of granular sludge from an upflow anaerobic sludge blanket (UASB) reactor treating brewery effluent was studied by denaturing gradient gel electrophoresis (DGGE). Twelve major bands were observed in the DGGE fingerprint for the Bacteria domain and four bands for the Archaea domain. Of the bacterial bands observed, six were successfully purified and sequenced. Among them, three were related to the gram-positive low G+C group, one to the Delta subclass of the Proteobacteria, one to the Gamma subclass, and one to the Cytophaga group with no close related sequence. The 16S rRNA sequences of the four archaeal bands were closely associated with Methanosaeta concilii and Methanobacterium formicum.
Persistent Identifierhttp://hdl.handle.net/10722/71422
ISSN
2015 Impact Factor: 1.064
2015 SCImago Journal Rankings: 0.469
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, OCen_HK
dc.contributor.authorLiu, WTen_HK
dc.contributor.authorFang, HHen_HK
dc.date.accessioned2010-09-06T06:31:51Z-
dc.date.available2010-09-06T06:31:51Z-
dc.date.issued2001en_HK
dc.identifier.citationWater Science And Technology, 2001, v. 43 n. 1, p. 77-82en_HK
dc.identifier.issn0273-1223en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71422-
dc.description.abstractThe microbial community structure of granular sludge from an upflow anaerobic sludge blanket (UASB) reactor treating brewery effluent was studied by denaturing gradient gel electrophoresis (DGGE). Twelve major bands were observed in the DGGE fingerprint for the Bacteria domain and four bands for the Archaea domain. Of the bacterial bands observed, six were successfully purified and sequenced. Among them, three were related to the gram-positive low G+C group, one to the Delta subclass of the Proteobacteria, one to the Gamma subclass, and one to the Cytophaga group with no close related sequence. The 16S rRNA sequences of the four archaeal bands were closely associated with Methanosaeta concilii and Methanobacterium formicum.en_HK
dc.languageengen_HK
dc.publisherIWA Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwsten_HK
dc.relation.ispartofWater Science and Technologyen_HK
dc.subject.meshBacteria, Anaerobic - geneticsen_HK
dc.subject.meshDNA Fingerprintingen_HK
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_HK
dc.subject.meshPopulation Dynamicsen_HK
dc.subject.meshRNA, Ribosomal, 16S - geneticsen_HK
dc.subject.meshWaste Disposal, Fluid - methodsen_HK
dc.titleStudy of microbial community of brewery-treating granular sludge by denaturing gradient gel electrophoresis of 16S rRNA geneen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0273-1223&volume=43 &issue=1&spage=77 &epage= 82&date=2001&atitle=Study+of+microbial+community+of+brewery-treating+granular+sludge+by+denaturing+gradient+gel+electrophoresis+of+16S+rRNA+geneen_HK
dc.identifier.emailFang, HH:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityFang, HH=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11379115-
dc.identifier.scopuseid_2-s2.0-0035113685en_HK
dc.identifier.hkuros60285en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035113685&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue1en_HK
dc.identifier.spage77en_HK
dc.identifier.epage82en_HK
dc.identifier.isiWOS:000167316800010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChan, OC=7004275779en_HK
dc.identifier.scopusauthoridLiu, WT=14029966400en_HK
dc.identifier.scopusauthoridFang, HH=7402542625en_HK

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