File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterization of nitrifying and denitrifying bacteria coimmobilized in PVA and kinetics model of biological nitrogen removal by coimmobilized cells

TitleCharacterization of nitrifying and denitrifying bacteria coimmobilized in PVA and kinetics model of biological nitrogen removal by coimmobilized cells
Authors
KeywordsBiologic nitrogen removal
Immobilized cell
Kinetics model
Issue Date2002
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictec
Citation
Enzyme And Microbial Technology, 2002, v. 30 n. 1, p. 49-55 How to Cite?
AbstractPolyvinyl alcohol (PVA) as a gel matrix was used to coimmobilize nitrifying and denitrifying bacteria by repeated freezing and thawing. Factors affecting single-stage biodenitrification (simultaneously occurring nitrification and denitrification in a reactor) process such as nitrifying bacteria and denitrifying bacteria ratio, organic carbon source, pH, alkalinity, temperature, dissolved oxygen (DO), and the operational stability of coimmobilized cells were investigated. Besides, the kinetics model of single-stage biodenitrification was studied. The experimental results showed that nitrogen removal rate was most fast when nitrifying to denitrifying bacteria ratio was between 1.5:1 and 3.6:1; in four carbon sources of methanol, ethanol, acetic acid and glucose, nitrogen removal rate was most fast when using ethanol; the optimal values of temperature, pH and DO were 30°C, 8.2 and 2∼6 mg · l -1, respectively; the higher alkalinity to ammonia ratio was, the faster nitrogen removal rate was, while nitrogen removal rate went steady when alkalinity to ammonia ratio is over 9. The continuous operational system remained stable for a period of more then 60 days, and the coimmobilized cells could easily adapt to an increase in loading. The effect of internal diffusion on overall rate could be neglected, and the limiting rate process was biochemical reaction in the single-stage biodenitrification process using coimmobilized cells. The reaction kinetics of coimmobilized cells followed Michaelis-Menten form, and its kinetics constant, K m and v max were 303.0 mg · l -1 and 0.096 mg · l -1 (immobilized cells) · s -1, respectively. The relative errors of predicted and experimental nitrogen removal rate were almost all less than 10%. © 2002 Elsevier Science Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/71335
ISSN
2015 Impact Factor: 2.624
2015 SCImago Journal Rankings: 0.854
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCao, GMen_HK
dc.contributor.authorZhao, QXen_HK
dc.contributor.authorSun, XBen_HK
dc.contributor.authorZhang, Ten_HK
dc.date.accessioned2010-09-06T06:31:04Z-
dc.date.available2010-09-06T06:31:04Z-
dc.date.issued2002en_HK
dc.identifier.citationEnzyme And Microbial Technology, 2002, v. 30 n. 1, p. 49-55en_HK
dc.identifier.issn0141-0229en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71335-
dc.description.abstractPolyvinyl alcohol (PVA) as a gel matrix was used to coimmobilize nitrifying and denitrifying bacteria by repeated freezing and thawing. Factors affecting single-stage biodenitrification (simultaneously occurring nitrification and denitrification in a reactor) process such as nitrifying bacteria and denitrifying bacteria ratio, organic carbon source, pH, alkalinity, temperature, dissolved oxygen (DO), and the operational stability of coimmobilized cells were investigated. Besides, the kinetics model of single-stage biodenitrification was studied. The experimental results showed that nitrogen removal rate was most fast when nitrifying to denitrifying bacteria ratio was between 1.5:1 and 3.6:1; in four carbon sources of methanol, ethanol, acetic acid and glucose, nitrogen removal rate was most fast when using ethanol; the optimal values of temperature, pH and DO were 30°C, 8.2 and 2∼6 mg · l -1, respectively; the higher alkalinity to ammonia ratio was, the faster nitrogen removal rate was, while nitrogen removal rate went steady when alkalinity to ammonia ratio is over 9. The continuous operational system remained stable for a period of more then 60 days, and the coimmobilized cells could easily adapt to an increase in loading. The effect of internal diffusion on overall rate could be neglected, and the limiting rate process was biochemical reaction in the single-stage biodenitrification process using coimmobilized cells. The reaction kinetics of coimmobilized cells followed Michaelis-Menten form, and its kinetics constant, K m and v max were 303.0 mg · l -1 and 0.096 mg · l -1 (immobilized cells) · s -1, respectively. The relative errors of predicted and experimental nitrogen removal rate were almost all less than 10%. © 2002 Elsevier Science Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictecen_HK
dc.relation.ispartofEnzyme and Microbial Technologyen_HK
dc.rightsEnzyme and Microbial Technology. Copyright © Elsevier Inc.en_HK
dc.subjectBiologic nitrogen removalen_HK
dc.subjectImmobilized cellen_HK
dc.subjectKinetics modelen_HK
dc.titleCharacterization of nitrifying and denitrifying bacteria coimmobilized in PVA and kinetics model of biological nitrogen removal by coimmobilized cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0141-0229&volume=30&spage=49&epage=55&date=2002&atitle=Characterization+of+nitrifying+and+denitrifying+bacteria+coimmobilized+in+PVA+and+kinetics+model+of+biological+nitrogen+removal+by+coimmobilized+cellsen_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0141-0229(01)00458-6en_HK
dc.identifier.scopuseid_2-s2.0-0037039509en_HK
dc.identifier.hkuros106170en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037039509&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue1en_HK
dc.identifier.spage49en_HK
dc.identifier.epage55en_HK
dc.identifier.isiWOS:000173306700008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCao, GM=7401710002en_HK
dc.identifier.scopusauthoridZhao, QX=7402764425en_HK
dc.identifier.scopusauthoridSun, XB=7405626508en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats