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Article: Quantification of extracellular polymeric substances in biofilms by confocal laser scanning microscopy

TitleQuantification of extracellular polymeric substances in biofilms by confocal laser scanning microscopy
Authors
Zhang, TFang, HHP
KeywordsBiofilm
Confocal
Extracellular
Microscopy
Polymer
Issue Date2001
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492
Citation
Biotechnology Letters, 2001, v. 23 n. 5, p. 405-409 How to Cite?
DOI: http://dx.doi.org/10.1023/A:1005620730265
AbstractExtracellular polymeric substances (EPS) in a biofilm were quantified by measuring the total cell volume from a 3-D image of the biofilm using confocal laser scanning microscope after staining cells with a fluorescent dye specific for nucleic acids. The EPS content was the difference between the volatile solids in the biofilm and the total cell mass, which could be quantified from the measured cell volume.
Persistent Identifierhttp://hdl.handle.net/10722/71274
ISSN
0141-5492
2021 Impact Factor: 2.716
2020 SCImago Journal Rankings: 0.548
ISI Accession Number ID
WOS:000166909800015
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2010-09-06T06:30:30Z-
dc.date.available2010-09-06T06:30:30Z-
dc.date.issued2001en_HK
dc.identifier.citationBiotechnology Letters, 2001, v. 23 n. 5, p. 405-409en_HK
dc.identifier.issn0141-5492en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71274-
dc.description.abstractExtracellular polymeric substances (EPS) in a biofilm were quantified by measuring the total cell volume from a 3-D image of the biofilm using confocal laser scanning microscope after staining cells with a fluorescent dye specific for nucleic acids. The EPS content was the difference between the volatile solids in the biofilm and the total cell mass, which could be quantified from the measured cell volume.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492en_HK
dc.relation.ispartofBiotechnology Lettersen_HK
dc.subjectBiofilmen_HK
dc.subjectConfocalen_HK
dc.subjectExtracellularen_HK
dc.subjectMicroscopyen_HK
dc.subjectPolymeren_HK
dc.titleQuantification of extracellular polymeric substances in biofilms by confocal laser scanning microscopyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0141-5492&volume=23&spage=405&epage=409&date=2001&atitle=Quantification+of+extracellular+polymeric+substances+in+biofilms+by+confocal+laser+scanning+microscopy.+en_HK
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hken_HK
dc.identifier.emailFang, HHP: hrechef@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1023/A:1005620730265en_HK
dc.identifier.scopuseid_2-s2.0-0035123630en_HK
dc.identifier.hkuros60275en_HK
dc.identifier.hkuros106420-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035123630&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue5en_HK
dc.identifier.spage405en_HK
dc.identifier.epage409en_HK
dc.identifier.isiWOS:000166909800015-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK
dc.identifier.issnl0141-5492-

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