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Article: Characterization of microbial community in granular sludge treating brewery wastewater

TitleCharacterization of microbial community in granular sludge treating brewery wastewater
Authors
Issue Date2002
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/watres
Citation
Water Research, 2002, v. 36 n. 7, p. 1767-1775 How to Cite?
AbstractThe diversity and distribution of microbes within brewery-degrading anaerobic sludge granules were studied using various molecular techniques. Molecular cloning of small-subunit rRNA gene sequences indicated that all archaeal clones were affiliated with Methanosaeta concillii (>99% sequence similarity), and the bacterial clones were mostly affiliated with a not-yet-cultured Clostridium cluster (48 out of 99 clones) in the low G+C gram-positive group, Xanthomonas spp. in the γ-subclass of Proteobacteria (30 clones), and Desulfovibrio spp. (16 clones) in the δ-subclass of Proteobacteria. Slot-blot hybridization indicated that archaeal cells from the Methanomicrobiales (58.4% of total rRNA), Methanobacterials (3.3%) and Methanococcales (1.0%) accounted for 62.4% of the total community rRNA. The rest of the microbial populations were the clostridial cluster (27.3% of total rRNA) and Desulfovibrio spp. (9.4%). Fluorescence in-situ hybridization with domain and group-specific oligonucleotide probes further revealed a multi-layer granular architecture. On the surface layer, the hydrolytic clostridial species and hydrogenotrophic Methanobacteriales were the predominant. In the middle layer, mostly H2-producing acetogens from the δ-Proteobacteria (i.e., Desulfovibrio spp.), hydrogenotrophic Methanobacteriales and aceticlastic Methanosaeta were observed to presumably form a syntrophic association. Finally, the center core consisted of microcolonies of Methanosaeta cells. Copyright © 2002 Elsevier Science Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/71126
ISSN
2015 Impact Factor: 5.991
2015 SCImago Journal Rankings: 2.772
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, WTen_HK
dc.contributor.authorChan, OCen_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2010-09-06T06:29:08Z-
dc.date.available2010-09-06T06:29:08Z-
dc.date.issued2002en_HK
dc.identifier.citationWater Research, 2002, v. 36 n. 7, p. 1767-1775en_HK
dc.identifier.issn0043-1354en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71126-
dc.description.abstractThe diversity and distribution of microbes within brewery-degrading anaerobic sludge granules were studied using various molecular techniques. Molecular cloning of small-subunit rRNA gene sequences indicated that all archaeal clones were affiliated with Methanosaeta concillii (>99% sequence similarity), and the bacterial clones were mostly affiliated with a not-yet-cultured Clostridium cluster (48 out of 99 clones) in the low G+C gram-positive group, Xanthomonas spp. in the γ-subclass of Proteobacteria (30 clones), and Desulfovibrio spp. (16 clones) in the δ-subclass of Proteobacteria. Slot-blot hybridization indicated that archaeal cells from the Methanomicrobiales (58.4% of total rRNA), Methanobacterials (3.3%) and Methanococcales (1.0%) accounted for 62.4% of the total community rRNA. The rest of the microbial populations were the clostridial cluster (27.3% of total rRNA) and Desulfovibrio spp. (9.4%). Fluorescence in-situ hybridization with domain and group-specific oligonucleotide probes further revealed a multi-layer granular architecture. On the surface layer, the hydrolytic clostridial species and hydrogenotrophic Methanobacteriales were the predominant. In the middle layer, mostly H2-producing acetogens from the δ-Proteobacteria (i.e., Desulfovibrio spp.), hydrogenotrophic Methanobacteriales and aceticlastic Methanosaeta were observed to presumably form a syntrophic association. Finally, the center core consisted of microcolonies of Methanosaeta cells. Copyright © 2002 Elsevier Science Ltd.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/watresen_HK
dc.relation.ispartofWater Researchen_HK
dc.subject.meshArchaea - classification - genetics - isolation & purificationen_HK
dc.subject.meshClostridium - classification - genetics - isolation & purificationen_HK
dc.subject.meshFood Industryen_HK
dc.subject.meshIn Situ Hybridization, Fluorescenceen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshProteobacteria - classification - genetics - isolation & purificationen_HK
dc.subject.meshRNA, Ribosomal - geneticsen_HK
dc.subject.meshSewage - microbiologyen_HK
dc.subject.meshWater Microbiologyen_HK
dc.subject.meshWater Purificationen_HK
dc.titleCharacterization of microbial community in granular sludge treating brewery wastewateren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0043-1354&volume=36 &issue=7&spage=1767 &epage= 1775&date=2002&atitle=Characterization+of+microbial+community+in+granular+sludge+treating+brewery+wastewateren_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0043-1354(01)00377-3en_HK
dc.identifier.pmid12044076-
dc.identifier.scopuseid_2-s2.0-0036216418en_HK
dc.identifier.hkuros67254en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036216418&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1767en_HK
dc.identifier.epage1775en_HK
dc.identifier.isiWOS:000175807400013-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLiu, WT=37045931100en_HK
dc.identifier.scopusauthoridChan, OC=7004275779en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK

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