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Article: Crystallization of oxytetracycline from fermentation waste liquor: Influence of biopolymer impurities

TitleCrystallization of oxytetracycline from fermentation waste liquor: Influence of biopolymer impurities
Authors
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jcis
Citation
Journal Of Colloid And Interface Science, 2004, v. 279 n. 1, p. 100-108 How to Cite?
AbstractOrganic impurities in the fermentation broth of antibiotic production impose great difficulties in the crystallization and recovery of antibiotics from the concentrated waste liquor. In the present laboratory study, the inhibitory effect of biopolymers on antibiotic crystallization was investigated using oxytetracycline (OTC) as the model antibiotic. Organic impurities separated from actual OTC fermentation waste liquor by ultrafiltration were dosed into a pure OTC solution at various concentrations. The results demonstrated that small organic molecules with an apparent molecular weight (AMW) of below 10,000 Da did not affect OTC crystallization significantly. However, large biopolymers, especially polysaccharides, in the fermentation waste caused severe retardation of crystal growth and considerable deterioration in the purity of the OTC crystallized. Atomic force microscopy (AFM) revealed that OTC nuclei formed in the solution attached to the surfaces of large organic molecules, probably polysaccharides, instead of being surrounded by proteins as previously thought. It is proposed that the attachment of OTC nuclei to biopolymers would prevent OTC from rapid crystallization, resulting in a high OTC residue in the aqueous phase. In addition, the adsorption of OTC clusters onto biopolymers would destabilize the colloidal system of organic macromolecules and promote particle flocculation. OTC crystallization would therefore take place with the precipitation of abundant organic impurities. Hence, the removal of polysaccharides and other biopolymers by ultrafiltration can be an effective means of improving the recovery of OTC and similar antibiotics by crystallization from the fermentation waste. © 2004 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/71116
ISSN
2015 Impact Factor: 3.782
2015 SCImago Journal Rankings: 1.126
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, SZen_HK
dc.contributor.authorLi, XYen_HK
dc.contributor.authorWang, Den_HK
dc.date.accessioned2010-09-06T06:29:03Z-
dc.date.available2010-09-06T06:29:03Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Colloid And Interface Science, 2004, v. 279 n. 1, p. 100-108en_HK
dc.identifier.issn0021-9797en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71116-
dc.description.abstractOrganic impurities in the fermentation broth of antibiotic production impose great difficulties in the crystallization and recovery of antibiotics from the concentrated waste liquor. In the present laboratory study, the inhibitory effect of biopolymers on antibiotic crystallization was investigated using oxytetracycline (OTC) as the model antibiotic. Organic impurities separated from actual OTC fermentation waste liquor by ultrafiltration were dosed into a pure OTC solution at various concentrations. The results demonstrated that small organic molecules with an apparent molecular weight (AMW) of below 10,000 Da did not affect OTC crystallization significantly. However, large biopolymers, especially polysaccharides, in the fermentation waste caused severe retardation of crystal growth and considerable deterioration in the purity of the OTC crystallized. Atomic force microscopy (AFM) revealed that OTC nuclei formed in the solution attached to the surfaces of large organic molecules, probably polysaccharides, instead of being surrounded by proteins as previously thought. It is proposed that the attachment of OTC nuclei to biopolymers would prevent OTC from rapid crystallization, resulting in a high OTC residue in the aqueous phase. In addition, the adsorption of OTC clusters onto biopolymers would destabilize the colloidal system of organic macromolecules and promote particle flocculation. OTC crystallization would therefore take place with the precipitation of abundant organic impurities. Hence, the removal of polysaccharides and other biopolymers by ultrafiltration can be an effective means of improving the recovery of OTC and similar antibiotics by crystallization from the fermentation waste. © 2004 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jcisen_HK
dc.relation.ispartofJournal of Colloid and Interface Scienceen_HK
dc.subject.meshBiopolymers - chemistryen_HK
dc.subject.meshCrystallizationen_HK
dc.subject.meshFermentationen_HK
dc.subject.meshIndustrial Wasteen_HK
dc.subject.meshMolecular Structureen_HK
dc.subject.meshOxytetracycline - chemistryen_HK
dc.subject.meshSurface Propertiesen_HK
dc.subject.meshTime Factorsen_HK
dc.titleCrystallization of oxytetracycline from fermentation waste liquor: Influence of biopolymer impuritiesen_HK
dc.typeArticleen_HK
dc.identifier.emailLi, XY:xlia@hkucc.hku.hken_HK
dc.identifier.authorityLi, XY=rp00222en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jcis.2004.06.050en_HK
dc.identifier.pmid15380417-
dc.identifier.scopuseid_2-s2.0-4544284936en_HK
dc.identifier.hkuros105746en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4544284936&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume279en_HK
dc.identifier.issue1en_HK
dc.identifier.spage100en_HK
dc.identifier.epage108en_HK
dc.identifier.isiWOS:000224708300011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, SZ=26660776400en_HK
dc.identifier.scopusauthoridLi, XY=26642887900en_HK
dc.identifier.scopusauthoridWang, D=7407068226en_HK

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