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Article: Protein profiles of extracellular polymeric substances and activated sludge in a membrane biological reactor by 2-dimensional gel electrophoresis

TitleProtein profiles of extracellular polymeric substances and activated sludge in a membrane biological reactor by 2-dimensional gel electrophoresis
Authors
KeywordsActivated sludge
Extracellular polymeric substances (EPS)
Membrane biological reactor (MBR)
Two-dimensional gel electrophoresis (2-DGE)
Issue Date2006
PublisherI W A Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwst_ws
Citation
Water Science And Technology: Water Supply, 2006, v. 6 n. 6, p. 27-33 How to Cite?
AbstractExtracellular polymeric substances (EPS) play a key role in wastewater treatment, specifically flocculation, settling and treatment efficiency. Proteins are the predominant components in EPS and influence hydrophobicity and flocculation of sludge. In this paper, two-dimensional gel electrophoresis (2-DGE) technique was used to analyse protein profiles of EPS from a membrane biological reactor (MBR) fed either glucose or acetate. A highly reproducible 2-DGE protocol was developed achieving >95% of protein spots matches in repeated runs. Results also showed that proteins of EPS obtained from the activated sludge displayed a similar protein pattern between pH 4-7 with molecular weight 10-80 kD. Protein profiles of EPS extracted from activated sludge fed glucose and acetate showed similar distribution patterns for proteins, but further separation of proteins between pH 4-7 revealed distinctive differences between the specific proteins from these two carbon sources. In addition, information of 2-DGE protein profiles indicates little relationship between the physical and chemical analyses. It is apparent that 2-DGE is a much more powerful and a very useful tool in studying metabolisms of microbial communities at the level of metaproteomics. © IWA Publishing 2006.
Persistent Identifierhttp://hdl.handle.net/10722/71011
ISSN
2015 Impact Factor: 0.532
2015 SCImago Journal Rankings: 0.315
References

 

DC FieldValueLanguage
dc.contributor.authorXie, Ben_HK
dc.contributor.authorGu, JDen_HK
dc.contributor.authorLi, XYen_HK
dc.date.accessioned2010-09-06T06:28:07Z-
dc.date.available2010-09-06T06:28:07Z-
dc.date.issued2006en_HK
dc.identifier.citationWater Science And Technology: Water Supply, 2006, v. 6 n. 6, p. 27-33en_HK
dc.identifier.issn1606-9749en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71011-
dc.description.abstractExtracellular polymeric substances (EPS) play a key role in wastewater treatment, specifically flocculation, settling and treatment efficiency. Proteins are the predominant components in EPS and influence hydrophobicity and flocculation of sludge. In this paper, two-dimensional gel electrophoresis (2-DGE) technique was used to analyse protein profiles of EPS from a membrane biological reactor (MBR) fed either glucose or acetate. A highly reproducible 2-DGE protocol was developed achieving >95% of protein spots matches in repeated runs. Results also showed that proteins of EPS obtained from the activated sludge displayed a similar protein pattern between pH 4-7 with molecular weight 10-80 kD. Protein profiles of EPS extracted from activated sludge fed glucose and acetate showed similar distribution patterns for proteins, but further separation of proteins between pH 4-7 revealed distinctive differences between the specific proteins from these two carbon sources. In addition, information of 2-DGE protein profiles indicates little relationship between the physical and chemical analyses. It is apparent that 2-DGE is a much more powerful and a very useful tool in studying metabolisms of microbial communities at the level of metaproteomics. © IWA Publishing 2006.en_HK
dc.languageengen_HK
dc.publisherI W A Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwst_wsen_HK
dc.relation.ispartofWater Science and Technology: Water Supplyen_HK
dc.subjectActivated sludgeen_HK
dc.subjectExtracellular polymeric substances (EPS)en_HK
dc.subjectMembrane biological reactor (MBR)en_HK
dc.subjectTwo-dimensional gel electrophoresis (2-DGE)en_HK
dc.titleProtein profiles of extracellular polymeric substances and activated sludge in a membrane biological reactor by 2-dimensional gel electrophoresisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1606-9749&volume=6&issue=6&spage=27&epage=33&date=2007&atitle=Protein+profiles+of+extracellular+polymeric+substances+and+activated+sludge+in+a+membrane+biological+reactor+by+2-dimensional+gel+electrophoresisen_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.emailLi, XY: xlia@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.identifier.authorityLi, XY=rp00222en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.2166/ws.2006.968en_HK
dc.identifier.scopuseid_2-s2.0-33847076515en_HK
dc.identifier.hkuros132045en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33847076515&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue6en_HK
dc.identifier.spage27en_HK
dc.identifier.epage33en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridXie, B=15849776300en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.scopusauthoridLi, XY=26642887900en_HK

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