File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: Use of P-17 and NOX specific primer sets for assimilable organic carbon (AOC) measurements

TitleUse of P-17 and NOX specific primer sets for assimilable organic carbon (AOC) measurements
Authors
KeywordsAOC
Assimilable organic carbon
Molecular technique
NOX
P-17
qRT-PCR
Issue Date2007
PublisherIWA Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwst_ws
Citation
International Symposium on Sustainable and Safe Water Supplies, Hong Kong, 15-16 January 2007. In Water Science And Technology: Water Supply, 2007, v. 7 n. 2, p. 157-163 How to Cite?
AbstractIn this study, two primer sets were at first designed specific to P-17 and NOX, the two bacterial species used for measurements of assimilable organic carbon (AOC) in water, based on their respective 16S rDNA sequences. These two primer sets were then used to measure the AOC content in 41 water samples by quantitative real time polymerase chain reaction (qRT-PCR). Results showed that P-17 and NOX cell numbers quantified by qRT-PCR using the designed primer sets were consistently higher (about 40.8% for P-17 and 54.8% for NOX measurements) than those by the traditional but time-consuming plate culture method. This proposed method not only saves the 5 days needed for plate culture, but also may detect AOC at concentrations as low as 0.3 μg-acetate-C/l and 0.6 μg-oxalate-C/l, which are lower than those measured by the plate culture and other methods. Using this new method, the water from the tap and a local reservoir were found containing 122 μg/l and 194 μg/l AOC, respectively, accounting for 5.7% and 7.8% of TOC. © IWA Publishing 2007.
DescriptionThis journal issue has title: "Sustainable and safe water supplies : selected proceedings of the international symposium on sustainable and safe water supplies, held in Hong Kong, China, 15-17 January 2007"
Persistent Identifierhttp://hdl.handle.net/10722/70944
ISSN
2015 Impact Factor: 0.532
2015 SCImago Journal Rankings: 0.315
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorQin, XLen_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2010-09-06T06:27:30Z-
dc.date.available2010-09-06T06:27:30Z-
dc.date.issued2007en_HK
dc.identifier.citationInternational Symposium on Sustainable and Safe Water Supplies, Hong Kong, 15-16 January 2007. In Water Science And Technology: Water Supply, 2007, v. 7 n. 2, p. 157-163en_HK
dc.identifier.issn1606-9749en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70944-
dc.descriptionThis journal issue has title: "Sustainable and safe water supplies : selected proceedings of the international symposium on sustainable and safe water supplies, held in Hong Kong, China, 15-17 January 2007"-
dc.description.abstractIn this study, two primer sets were at first designed specific to P-17 and NOX, the two bacterial species used for measurements of assimilable organic carbon (AOC) in water, based on their respective 16S rDNA sequences. These two primer sets were then used to measure the AOC content in 41 water samples by quantitative real time polymerase chain reaction (qRT-PCR). Results showed that P-17 and NOX cell numbers quantified by qRT-PCR using the designed primer sets were consistently higher (about 40.8% for P-17 and 54.8% for NOX measurements) than those by the traditional but time-consuming plate culture method. This proposed method not only saves the 5 days needed for plate culture, but also may detect AOC at concentrations as low as 0.3 μg-acetate-C/l and 0.6 μg-oxalate-C/l, which are lower than those measured by the plate culture and other methods. Using this new method, the water from the tap and a local reservoir were found containing 122 μg/l and 194 μg/l AOC, respectively, accounting for 5.7% and 7.8% of TOC. © IWA Publishing 2007.en_HK
dc.languageengen_HK
dc.publisherIWA Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwst_wsen_HK
dc.relation.ispartofWater Science and Technology: Water Supplyen_HK
dc.subjectAOCen_HK
dc.subjectAssimilable organic carbonen_HK
dc.subjectMolecular techniqueen_HK
dc.subjectNOXen_HK
dc.subjectP-17en_HK
dc.subjectqRT-PCRen_HK
dc.titleUse of P-17 and NOX specific primer sets for assimilable organic carbon (AOC) measurementsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1606-9749&volume=7&issue=2&spage=157&epage=163&date=2007&atitle=Use+of+P-17+and+NOX+specific+primer+sets+for+assimilable+organic+carbon+(AOC)+measurements-
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.2166/ws.2007.050en_HK
dc.identifier.scopuseid_2-s2.0-34548162599en_HK
dc.identifier.hkuros143155en_HK
dc.identifier.hkuros175705-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548162599&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue2en_HK
dc.identifier.spage157en_HK
dc.identifier.epage163en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.description.otherInternational Symposium on Sustainable and Safe Water Supplies, Hong Kong, 15-16 January 2007. In Water Science And Technology: Water Supply, 2007, v. 7 n. 2, p. 157-163-
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridQin, XL=19934218600en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats