Article: 16S rDNA clone library screening of environmental sample using melting curve analysis

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Title16S rDNA clone library screening of environmental sample using melting curve analysis
AuthorsZhang, T1
Fang, HHP1
Keywords16S rDNA
Clone library
Melting curve analysis
Issue Date2005
PublisherTaiwan.
CitationJournal Of The Chinese Institute Of Engineers, Transactions Of The Chinese Institute Of Engineers,Series A/Chung-Kuo Kung Ch'eng Hsuch K'an, 2005, v. 28 n. 7, p. 1153-1155 [How to Cite?]
AbstractA new method of screening a 16S rDNA clone library from an environmental sample is demonstrated using the non-specific fluorescent dye SYBR Green for the melting curve analysis of PCR-amplified DNA fragments. It is simple, easy and swift to operate. In addition, it requires little manual operation and the product may be still used for further analysis.
ISSN0253-3839
2011 Impact Factor: 0.295
2011 SCImago Journal Rankings: 0.031
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorZhang, T
dc.contributor.authorFang, HHP
dc.date.accessioned2010-09-06T06:26:28Z
dc.date.available2010-09-06T06:26:28Z
dc.date.issued2005
dc.description.abstractA new method of screening a 16S rDNA clone library from an environmental sample is demonstrated using the non-specific fluorescent dye SYBR Green for the melting curve analysis of PCR-amplified DNA fragments. It is simple, easy and swift to operate. In addition, it requires little manual operation and the product may be still used for further analysis.
dc.description.naturelink_to_subscribed_fulltext
dc.identifier.citationJournal Of The Chinese Institute Of Engineers, Transactions Of The Chinese Institute Of Engineers,Series A/Chung-Kuo Kung Ch'eng Hsuch K'an, 2005, v. 28 n. 7, p. 1153-1155 [How to Cite?]
dc.identifier.epage1155
dc.identifier.hkuros104524
dc.identifier.hkuros120538
dc.identifier.issn0253-3839
2011 Impact Factor: 0.295
2011 SCImago Journal Rankings: 0.031
dc.identifier.issue7
dc.identifier.scopuseid_2-s2.0-28744434906
dc.identifier.spage1153
dc.identifier.urihttp://hdl.handle.net/10722/70827
dc.identifier.volume28
dc.languageeng
dc.publisherTaiwan.
dc.relation.ispartofJournal of the Chinese Institute of Engineers, Transactions of the Chinese Institute of Engineers,Series A/Chung-kuo Kung Ch'eng Hsuch K'an
dc.relation.referencesReferences in Scopus
dc.subject16S rDNA
dc.subjectClone library
dc.subjectMelting curve analysis
dc.title16S rDNA clone library screening of environmental sample using melting curve analysis
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong