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Article: 16S rDNA clone library screening of environmental sample using melting curve analysis

Title16S rDNA clone library screening of environmental sample using melting curve analysis
Authors
Keywords16S rDNA
Clone library
Melting curve analysis
Issue Date2005
PublisherTaiwan.
Citation
Journal Of The Chinese Institute Of Engineers, Transactions Of The Chinese Institute Of Engineers,Series A/Chung-Kuo Kung Ch'eng Hsuch K'an, 2005, v. 28 n. 7, p. 1153-1155 How to Cite?
Abstract
A new method of screening a 16S rDNA clone library from an environmental sample is demonstrated using the non-specific fluorescent dye SYBR Green for the melting curve analysis of PCR-amplified DNA fragments. It is simple, easy and swift to operate. In addition, it requires little manual operation and the product may be still used for further analysis.
Persistent Identifierhttp://hdl.handle.net/10722/70827
ISSN
2013 Impact Factor: 0.209
2013 SCImago Journal Rankings: 0.225
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2010-09-06T06:26:28Z-
dc.date.available2010-09-06T06:26:28Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of The Chinese Institute Of Engineers, Transactions Of The Chinese Institute Of Engineers,Series A/Chung-Kuo Kung Ch'eng Hsuch K'an, 2005, v. 28 n. 7, p. 1153-1155en_HK
dc.identifier.issn0253-3839en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70827-
dc.description.abstractA new method of screening a 16S rDNA clone library from an environmental sample is demonstrated using the non-specific fluorescent dye SYBR Green for the melting curve analysis of PCR-amplified DNA fragments. It is simple, easy and swift to operate. In addition, it requires little manual operation and the product may be still used for further analysis.en_HK
dc.languageengen_HK
dc.publisherTaiwan.en_HK
dc.relation.ispartofJournal of the Chinese Institute of Engineers, Transactions of the Chinese Institute of Engineers,Series A/Chung-kuo Kung Ch'eng Hsuch K'anen_HK
dc.subject16S rDNAen_HK
dc.subjectClone libraryen_HK
dc.subjectMelting curve analysisen_HK
dc.title16S rDNA clone library screening of environmental sample using melting curve analysisen_HK
dc.typeArticleen_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-28744434906en_HK
dc.identifier.hkuros104524en_HK
dc.identifier.hkuros120538-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-28744434906&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1153en_HK
dc.identifier.epage1155en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK

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