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Article: Microbial diversity of a mesophilic hydrogen-producing sludge

TitleMicrobial diversity of a mesophilic hydrogen-producing sludge
Authors
Issue Date2002
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology And Biotechnology, 2002, v. 58 n. 1, p. 112-118 How to Cite?
AbstractA hydrogen-producing sludge degraded 99% of glucose at 36°C and pH 5.5, producing a methane-free biogas (comprising 64% hydrogen) and an effluent comprising mostly butyrate, acetate, and ethanol. The yield was 0.26 1 H2 g-1 glucose and the production rate per gram of volatile suspended solids was 4.6 1 H2 day-1. A 16S rDNA library was constructed from the sludge for microbial species determination. A total of 96 clones were selected for plasmids recovery, screened by denaturing gradient gel electrophoresis, and sequenced for rDNA. Based on the phylogenetic analysis of the rDNA sequences, 64.6% of all the clones were affiliated with three Clostridium species (Clostridiaceae), 18.8% with Enterobacteriaceae, and 3.1% with Streptococcus bovis (Streptococcaceae). The remaining 13.5% belonged to eight operational taxonomic units, the affiliations of which were not identified.
Persistent Identifierhttp://hdl.handle.net/10722/70779
ISSN
2015 Impact Factor: 3.376
2015 SCImago Journal Rankings: 1.262
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFang, HHPen_HK
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorLiu, Hen_HK
dc.date.accessioned2010-09-06T06:26:03Z-
dc.date.available2010-09-06T06:26:03Z-
dc.date.issued2002en_HK
dc.identifier.citationApplied Microbiology And Biotechnology, 2002, v. 58 n. 1, p. 112-118en_HK
dc.identifier.issn0175-7598en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70779-
dc.description.abstractA hydrogen-producing sludge degraded 99% of glucose at 36°C and pH 5.5, producing a methane-free biogas (comprising 64% hydrogen) and an effluent comprising mostly butyrate, acetate, and ethanol. The yield was 0.26 1 H2 g-1 glucose and the production rate per gram of volatile suspended solids was 4.6 1 H2 day-1. A 16S rDNA library was constructed from the sludge for microbial species determination. A total of 96 clones were selected for plasmids recovery, screened by denaturing gradient gel electrophoresis, and sequenced for rDNA. Based on the phylogenetic analysis of the rDNA sequences, 64.6% of all the clones were affiliated with three Clostridium species (Clostridiaceae), 18.8% with Enterobacteriaceae, and 3.1% with Streptococcus bovis (Streptococcaceae). The remaining 13.5% belonged to eight operational taxonomic units, the affiliations of which were not identified.en_HK
dc.languageengen_HK
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htmen_HK
dc.relation.ispartofApplied Microbiology and Biotechnologyen_HK
dc.subject.meshBacteria - classification - genetics - isolation & purification - metabolismen_HK
dc.subject.meshBioreactorsen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshDNA, Bacterial - analysis - geneticsen_HK
dc.subject.meshDNA, Ribosomal - analysis - geneticsen_HK
dc.subject.meshHydrogen - metabolismen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshRNA, Ribosomal, 16S - geneticsen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSewage - microbiologyen_HK
dc.titleMicrobial diversity of a mesophilic hydrogen-producing sludgeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0175-7598&volume=58&spage=112&epage=118&date=2002&atitle=Microbial+diversity+of+a+mesophilic+hydrogen-producing+sludge.+en_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00253-001-0865-8en_HK
dc.identifier.pmid11833529-
dc.identifier.scopuseid_2-s2.0-0036172432en_HK
dc.identifier.hkuros106174en_HK
dc.identifier.hkuros67259-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036172432&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume58en_HK
dc.identifier.issue1en_HK
dc.identifier.spage112en_HK
dc.identifier.epage118en_HK
dc.identifier.isiWOS:000173559200016-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridLiu, H=36072584800en_HK

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