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Article: Microbial characterization and quantification of an anaerobic sludge degrading dimethyl phthalate

TitleMicrobial characterization and quantification of an anaerobic sludge degrading dimethyl phthalate
Authors
Issue Date2009
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JAM
Citation
Journal Of Applied Microbiology, 2009, v. 106 n. 1, p. 296-305 How to Cite?
AbstractAims: Characterization and quantification of microbial community in dimethyl phthalate (DMP)-degrading anaerobic sludge using molecular techniques. Methods and Results: An enriched anaerobic sludge effectively degrading over 99% of dimethyl phthalate in an upflow anaerobic sludge blanket (UASB) reactor for 530 days was characterized and quantified by 16S rRNA-based molecular methods. A total of 78 Bacteria clones were classified into 22 operational taxonomic units (OTUs) in nine divisions, including Firmicutes, Proteobacteria, Chloroflexi, Thermotogae, Bacteroidetes/Chlorobi, Spirochaetes, Acidobacteria and two candidate divisions. The two most abundant OTUs were likely responsible, respectively, for the de-esterification of DMP and the subsequent phthalate degradation. The outer layer of the granule was dominated by Bacteria; whereas the interior was by Archaea, of which 89 ± 5% were acetoclastic Methanosaetaceae and 11 ± 5% hydrogenotrophic Methanomicrobiales. Conclusions: Twenty-two Bacteria OTUs in DMP-degrading anaerobic sludge distributed in nine divisions. The two most abundant OTUs were likely responsible respectively for the de-esterification of DMP and the subsequent phthalate degradation. Layered granular microstructure of DMP-degrading anaerobic sludge suggested that the rate of DMP de-esterification is faster than its inward diffusion rate. Significance and Impact of the Study: This work is the first study to characterize and quantify the microbial community in the anaerobic phthalic ester degrading sludge from the anaerobic reactor. © 2008 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/70561
ISSN
2015 Impact Factor: 2.156
2015 SCImago Journal Rankings: 0.921
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7107/03E
Funding Information:

The authors wish to thank the Hong Kong Research Grants Council for the financial support of this study (HKU 7107/03E), and D.W. Liang wishes to thank The University of Hong Kong for the postgraduate studentship.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLiang, DWen_HK
dc.contributor.authorFang, HHPen_HK
dc.contributor.authorZhang, Ten_HK
dc.date.accessioned2010-09-06T06:24:05Z-
dc.date.available2010-09-06T06:24:05Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Applied Microbiology, 2009, v. 106 n. 1, p. 296-305en_HK
dc.identifier.issn1364-5072en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70561-
dc.description.abstractAims: Characterization and quantification of microbial community in dimethyl phthalate (DMP)-degrading anaerobic sludge using molecular techniques. Methods and Results: An enriched anaerobic sludge effectively degrading over 99% of dimethyl phthalate in an upflow anaerobic sludge blanket (UASB) reactor for 530 days was characterized and quantified by 16S rRNA-based molecular methods. A total of 78 Bacteria clones were classified into 22 operational taxonomic units (OTUs) in nine divisions, including Firmicutes, Proteobacteria, Chloroflexi, Thermotogae, Bacteroidetes/Chlorobi, Spirochaetes, Acidobacteria and two candidate divisions. The two most abundant OTUs were likely responsible, respectively, for the de-esterification of DMP and the subsequent phthalate degradation. The outer layer of the granule was dominated by Bacteria; whereas the interior was by Archaea, of which 89 ± 5% were acetoclastic Methanosaetaceae and 11 ± 5% hydrogenotrophic Methanomicrobiales. Conclusions: Twenty-two Bacteria OTUs in DMP-degrading anaerobic sludge distributed in nine divisions. The two most abundant OTUs were likely responsible respectively for the de-esterification of DMP and the subsequent phthalate degradation. Layered granular microstructure of DMP-degrading anaerobic sludge suggested that the rate of DMP de-esterification is faster than its inward diffusion rate. Significance and Impact of the Study: This work is the first study to characterize and quantify the microbial community in the anaerobic phthalic ester degrading sludge from the anaerobic reactor. © 2008 The Authors.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JAMen_HK
dc.relation.ispartofJournal of Applied Microbiologyen_HK
dc.rightsJournal of Applied Microbiology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subject.meshArchaea - classification - genetics - growth & development - metabolismen_HK
dc.subject.meshBacteria, Anaerobic - classification - genetics - growth & development - metabolismen_HK
dc.subject.meshBioreactorsen_HK
dc.subject.meshDNA, Archaeal - analysisen_HK
dc.subject.meshDNA, Bacterial - analysisen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPhthalic Acids - metabolismen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshRNA, Ribosomal, 16S - analysisen_HK
dc.subject.meshSewage - microbiologyen_HK
dc.titleMicrobial characterization and quantification of an anaerobic sludge degrading dimethyl phthalateen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1364-5072&volume=106&spage=296&epage=305 &date=2008&atitle=Microbial+characterization+and+quantification+of+an+anaerobic+sludge+degrading+dimethyl+phthalateen_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1365-2672.2008.04003.xen_HK
dc.identifier.pmid19120614-
dc.identifier.scopuseid_2-s2.0-58249114976en_HK
dc.identifier.hkuros155502en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58249114976&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume106en_HK
dc.identifier.issue1en_HK
dc.identifier.spage296en_HK
dc.identifier.epage305en_HK
dc.identifier.isiWOS:000262475600031-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectBiodegradation of endocrine disrupters in landfill leachate-
dc.identifier.scopusauthoridLiang, DW=15835235400en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK

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