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Article: Applications of real-time polymerase chain reaction for quantification of microorganisms in environmental samples

TitleApplications of real-time polymerase chain reaction for quantification of microorganisms in environmental samples
Authors
Issue Date2006
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology And Biotechnology, 2006, v. 70 n. 3, p. 281-289 How to Cite?
AbstractDue to the advanced development of fluorogenic chemistry, quantitative real-time polymerase chain reaction (qRT-PCR) has become an emerging technique for the detection and quantification of microorganisms in the environment. Compared with the conventional hybridization- and PCR-based techniques, qRT-PCR not only has better sensitivity and reproducibility, but it is also quicker to perform and has a minimum risk of amplicon carryover contamination. This article reviews the principle of this emerging technique, its detection reagents, target DNAs, quantification procedures, and affecting factors. The applications of qRT-PCR for the quantification of microorganisms in the environment are also summarized. © Springer-Verlag 2006.
Persistent Identifierhttp://hdl.handle.net/10722/70558
ISSN
2015 Impact Factor: 3.376
2015 SCImago Journal Rankings: 1.262
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2010-09-06T06:24:03Z-
dc.date.available2010-09-06T06:24:03Z-
dc.date.issued2006en_HK
dc.identifier.citationApplied Microbiology And Biotechnology, 2006, v. 70 n. 3, p. 281-289en_HK
dc.identifier.issn0175-7598en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70558-
dc.description.abstractDue to the advanced development of fluorogenic chemistry, quantitative real-time polymerase chain reaction (qRT-PCR) has become an emerging technique for the detection and quantification of microorganisms in the environment. Compared with the conventional hybridization- and PCR-based techniques, qRT-PCR not only has better sensitivity and reproducibility, but it is also quicker to perform and has a minimum risk of amplicon carryover contamination. This article reviews the principle of this emerging technique, its detection reagents, target DNAs, quantification procedures, and affecting factors. The applications of qRT-PCR for the quantification of microorganisms in the environment are also summarized. © Springer-Verlag 2006.en_HK
dc.languageengen_HK
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htmen_HK
dc.relation.ispartofApplied Microbiology and Biotechnologyen_HK
dc.subject.meshArchaea - genetics - isolation & purificationen_HK
dc.subject.meshBacteria - genetics - isolation & purificationen_HK
dc.subject.meshDNA, Archaeal - analysisen_HK
dc.subject.meshDNA, Bacterial - analysisen_HK
dc.subject.meshEnvironmental Microbiologyen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.titleApplications of real-time polymerase chain reaction for quantification of microorganisms in environmental samplesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0175-7598&volume=70&spage=281&epage=289&date=2006&atitle=Applications+of+real-time+polymerase+chain+reaction+for+quantification+of+microorganisms+in+environmental+samplesen_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00253-006-0333-6en_HK
dc.identifier.pmid16470363-
dc.identifier.scopuseid_2-s2.0-33644776725en_HK
dc.identifier.hkuros116006en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33644776725&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume70en_HK
dc.identifier.issue3en_HK
dc.identifier.spage281en_HK
dc.identifier.epage289en_HK
dc.identifier.isiWOS:000235865600003-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK
dc.identifier.citeulike6462995-

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