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Article: Microchip capillary electrophoresis for frontal analysis of free bilirubin and study of its interaction with human serum albumin

TitleMicrochip capillary electrophoresis for frontal analysis of free bilirubin and study of its interaction with human serum albumin
Authors
KeywordsBilirubin
Frontal analysis
Human serum albumin
Microchip capillary electrophoresis
Multichannel microfluidic chip
Issue Date2008
PublisherWiley - V C H Verlag GmbH & Co KGaA.
Citation
Electrophoresis, 2008, v. 29 n. 9, p. 1924-1931 How to Cite?
AbstractTo meet the need for bedside monitoring of free bilirubin for neonates under critical conditions, a microfluidic chip was fabricated and tested for its coupling with CE/frontal analysis (FA) to determine free bilirubin and study of its binding interaction with HSA, which regulated its concentration in plasma. The poly(methyl methacrylate) (PMMA) multichannel chip was fabricated by CO2 laser ablation and bonded with a fused-silica separation capillary for CE/FA separation with UV detection. The chip was designed to allow a complete assay of four electrophoretic runs using preconditioned channels to speed up the determination of free bilirubin and to deliver quick results for bedside monitoring. Under optimized conditions, the linear working range for free bilirubin was from 10 to 200 μmol with RSDs from 2.1 to 5.0% for n = 3, and the LOD at 9 μmol for S/N = 3. From a binding study between bilirubin and HSA under FA condition, the second binding constant for bilirubin-HSA was determined as 1.07 × 105 L/mol and the number of binding sites per HSA as 3.46. The results enabled the calculation of free bilirubin for jaundiced infants based on the clinically significant level of total bilirubin, producing a range of 118.3-119.4 μmol/L. The developed method is shown to meet the clinical requirement with additional margin of protection to detect the early rising level of free bilirubin prior to jaundice condition. The low-cost microchip CE/FA device is shown to produce quick results with high potential to deliver a suitable bed-side monitoring method for bilirubin management in neonates. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/70444
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNie, Zen_HK
dc.contributor.authorFung, YSen_HK
dc.date.accessioned2010-09-06T06:22:56Z-
dc.date.available2010-09-06T06:22:56Z-
dc.date.issued2008en_HK
dc.identifier.citationElectrophoresis, 2008, v. 29 n. 9, p. 1924-1931en_HK
dc.identifier.issn0173-0835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70444-
dc.description.abstractTo meet the need for bedside monitoring of free bilirubin for neonates under critical conditions, a microfluidic chip was fabricated and tested for its coupling with CE/frontal analysis (FA) to determine free bilirubin and study of its binding interaction with HSA, which regulated its concentration in plasma. The poly(methyl methacrylate) (PMMA) multichannel chip was fabricated by CO2 laser ablation and bonded with a fused-silica separation capillary for CE/FA separation with UV detection. The chip was designed to allow a complete assay of four electrophoretic runs using preconditioned channels to speed up the determination of free bilirubin and to deliver quick results for bedside monitoring. Under optimized conditions, the linear working range for free bilirubin was from 10 to 200 μmol with RSDs from 2.1 to 5.0% for n = 3, and the LOD at 9 μmol for S/N = 3. From a binding study between bilirubin and HSA under FA condition, the second binding constant for bilirubin-HSA was determined as 1.07 × 105 L/mol and the number of binding sites per HSA as 3.46. The results enabled the calculation of free bilirubin for jaundiced infants based on the clinically significant level of total bilirubin, producing a range of 118.3-119.4 μmol/L. The developed method is shown to meet the clinical requirement with additional margin of protection to detect the early rising level of free bilirubin prior to jaundice condition. The low-cost microchip CE/FA device is shown to produce quick results with high potential to deliver a suitable bed-side monitoring method for bilirubin management in neonates. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA.en_HK
dc.relation.ispartofElectrophoresisen_HK
dc.subjectBilirubinen_HK
dc.subjectFrontal analysisen_HK
dc.subjectHuman serum albuminen_HK
dc.subjectMicrochip capillary electrophoresisen_HK
dc.subjectMultichannel microfluidic chipen_HK
dc.titleMicrochip capillary electrophoresis for frontal analysis of free bilirubin and study of its interaction with human serum albuminen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0173-0835&volume=29&spage=1924&epage=1931&date=2008&atitle=Microchip+Capillary+Electrophoresis+for+Frontal+Analysis+of+Free+Bilirubin+and+Study+of+its+Interaction+with+Human+Serum+Albumin+en_HK
dc.identifier.emailFung, YS:ysfung@hku.hken_HK
dc.identifier.authorityFung, YS=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/elps.200700596en_HK
dc.identifier.pmid18393342-
dc.identifier.scopuseid_2-s2.0-43449129182en_HK
dc.identifier.hkuros145383en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-43449129182&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1924en_HK
dc.identifier.epage1931en_HK
dc.identifier.isiWOS:000256510000020-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridNie, Z=24177229900en_HK
dc.identifier.scopusauthoridFung, YS=13309754700en_HK

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