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Article: All-trans retinoic acid induces XAF1 expression through an interferon regulatory factor-1 element in colon cancer

TitleAll-trans retinoic acid induces XAF1 expression through an interferon regulatory factor-1 element in colon cancer
Authors
Issue Date2006
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
Gastroenterology, 2006, v. 130 n. 3, p. 747-758 How to Cite?
AbstractBackground & Aims: X-linked inhibitor of apoptosis protein (XIAP)-associated factor 1 (XAF1) is a novel tumor suppressor and interferon (IFN)-stimulated gene. All-trans retinoic acid (ATRA) exerts an antiproliferative effect on tumor cells through up-regulation of IFN regulatory factor 1 (IRF-1) and the downstream IFN-stimulated genes. The aim of this study was to determine the effect and mechanism of ATRA on XAF1 expression and the role of XAF1 in ATRA-induced growth inhibition in colon cancer. Methods: Gene expression is detected by reverse-transcription polymerase chain reaction and immunoblotting. The transcription activity of XAF1 promoter is examined by luciferase reporter assay. The activity of IFN regulatory factor binding element (IRF-E) is assessed by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Cell growth is evaluated by both in vitro and in vivo in nude mice xenografts. Results: IFN-alfa stimulates XAF1 promoter activity in the colon cancer cells Lovo and SW1116 dose-dependently. An IRF-1 binding element (IRF-E-XAF1) is found in the -30 to -38 nucleotide region upstream of the ATG initiator codon of the XAF1 gene. Site-directed mutagenesis of IRF-E-XAF1 abrogates native and IFN-induced promoter activity and binding capacity. ATRA induces XAF1 expression both in vitro and in vivo through interaction with IRF-E-XAF1. Overexpression of XAF1 increases cell susceptibility to ATRA-induced growth suppression both in vitro and in vivo. Furthermore, the effect of ATRA on XAF1 expression is independent of the promoter methylation and the subcellular distribution of XIAP. Conclusions: XAF1 participates in ATRA-induced growth suppression through IRF-1-mediated transcriptional regulation. © 2006 by the American Gastroenterological Association Institute.
Persistent Identifierhttp://hdl.handle.net/10722/70433
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Jen_HK
dc.contributor.authorPeng, Yen_HK
dc.contributor.authorSun, YWen_HK
dc.contributor.authorHe, Hen_HK
dc.contributor.authorZhu, Sen_HK
dc.contributor.authorAn, Xen_HK
dc.contributor.authorLi, Men_HK
dc.contributor.authorLin, MCMen_HK
dc.contributor.authorZou, Ben_HK
dc.contributor.authorXia, HHXen_HK
dc.contributor.authorJiang, Ben_HK
dc.contributor.authorChan, AOOen_HK
dc.contributor.authorYuen, MFen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2010-09-06T06:22:50Z-
dc.date.available2010-09-06T06:22:50Z-
dc.date.issued2006en_HK
dc.identifier.citationGastroenterology, 2006, v. 130 n. 3, p. 747-758en_HK
dc.identifier.issn0016-5085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70433-
dc.description.abstractBackground & Aims: X-linked inhibitor of apoptosis protein (XIAP)-associated factor 1 (XAF1) is a novel tumor suppressor and interferon (IFN)-stimulated gene. All-trans retinoic acid (ATRA) exerts an antiproliferative effect on tumor cells through up-regulation of IFN regulatory factor 1 (IRF-1) and the downstream IFN-stimulated genes. The aim of this study was to determine the effect and mechanism of ATRA on XAF1 expression and the role of XAF1 in ATRA-induced growth inhibition in colon cancer. Methods: Gene expression is detected by reverse-transcription polymerase chain reaction and immunoblotting. The transcription activity of XAF1 promoter is examined by luciferase reporter assay. The activity of IFN regulatory factor binding element (IRF-E) is assessed by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Cell growth is evaluated by both in vitro and in vivo in nude mice xenografts. Results: IFN-alfa stimulates XAF1 promoter activity in the colon cancer cells Lovo and SW1116 dose-dependently. An IRF-1 binding element (IRF-E-XAF1) is found in the -30 to -38 nucleotide region upstream of the ATG initiator codon of the XAF1 gene. Site-directed mutagenesis of IRF-E-XAF1 abrogates native and IFN-induced promoter activity and binding capacity. ATRA induces XAF1 expression both in vitro and in vivo through interaction with IRF-E-XAF1. Overexpression of XAF1 increases cell susceptibility to ATRA-induced growth suppression both in vitro and in vivo. Furthermore, the effect of ATRA on XAF1 expression is independent of the promoter methylation and the subcellular distribution of XIAP. Conclusions: XAF1 participates in ATRA-induced growth suppression through IRF-1-mediated transcriptional regulation. © 2006 by the American Gastroenterological Association Institute.en_HK
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastroen_HK
dc.relation.ispartofGastroenterologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshColonic Neoplasms - drug therapy - metabolismen_HK
dc.subject.meshCpG Islandsen_HK
dc.subject.meshDNA Methylationen_HK
dc.subject.meshGene Expression Regulation - drug effectsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInterferon Regulatory Factor-1 - physiologyen_HK
dc.subject.meshIntracellular Signaling Peptides and Proteinsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMutagenesis, Site-Directeden_HK
dc.subject.meshNeoplasm Proteins - genetics - physiologyen_HK
dc.subject.meshPromoter Regions, Geneticen_HK
dc.subject.meshTranscription Initiation Siteen_HK
dc.subject.meshTretinoin - pharmacologyen_HK
dc.titleAll-trans retinoic acid induces XAF1 expression through an interferon regulatory factor-1 element in colon canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-5085&volume=130&issue=3&spage=747&epage=758&date=2006&atitle=All-Trans+Retinoic+Acid+Induces+XAF1+Expression+Through+an+Interferon+Regulatory+Factor-1+Element+in+Colon+Canceren_HK
dc.identifier.emailWang, J: jidewang@gmail.comen_HK
dc.identifier.emailLin, MCM: mcllin@hkucc.hku.hken_HK
dc.identifier.emailYuen, MF: mfyuen@hku.hken_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.authorityWang, J=rp00491en_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.identifier.authorityYuen, MF=rp00479en_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1053/j.gastro.2005.12.017en_HK
dc.identifier.pmid16530516-
dc.identifier.scopuseid_2-s2.0-33644868920en_HK
dc.identifier.hkuros114944en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33644868920&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume130en_HK
dc.identifier.issue3en_HK
dc.identifier.spage747en_HK
dc.identifier.epage758en_HK
dc.identifier.isiWOS:000236210100019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, J=35309087500en_HK
dc.identifier.scopusauthoridPeng, Y=7403419265en_HK
dc.identifier.scopusauthoridSun, YW=12776261000en_HK
dc.identifier.scopusauthoridHe, H=36185495900en_HK
dc.identifier.scopusauthoridZhu, S=7404391208en_HK
dc.identifier.scopusauthoridAn, X=12774780700en_HK
dc.identifier.scopusauthoridLi, M=36067425800en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK
dc.identifier.scopusauthoridZou, B=35228257300en_HK
dc.identifier.scopusauthoridXia, HHX=8757161400en_HK
dc.identifier.scopusauthoridJiang, B=34770534200en_HK
dc.identifier.scopusauthoridChan, AOO=7403167965en_HK
dc.identifier.scopusauthoridYuen, MF=7102031955en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK

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