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Article: Ganoderma lucidum mycelium and spore extracts as natural adjuvants for immunotherapy

TitleGanoderma lucidum mycelium and spore extracts as natural adjuvants for immunotherapy
Authors
Issue Date2005
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/acm
Citation
Journal Of Alternative And Complementary Medicine, 2005, v. 11 n. 6, p. 1047-1057 How to Cite?
AbstractObjectives: Ganoderma lucidum (GL) is one of the most commonly used Chinese herbs in the oriental community, with more than 30% of pediatric cancer patients taking GL. The immunomodulating and anticancer effects exerted by GL extracts have been demonstrated by in vitro and in vivo studies. There was, however, no comparison between the immunomodulating effects of GL mycelium extract (GL-M) and spore extracts on human immune cells. Dendritic cells (DCs) are professional antigen-presenting cells and their role in DC-based tumor vaccine has been well defined. The possibility of GL as natural adjuvant for human DCs remains unknown. Design: This study explored the differential effect of GL-M and GL spore extract (GL-S) on proliferation and Th1/Th2 cytokine mRNA expression of human peripheral blood mononuclear cells (PBMCs) and monocytes. Their effects on the phenotypic and functional maturation of human monocyte-derived DCs were also investigated. Results: GL-M induced the proliferation of PBMCs and monocytes, whereas GL-S showed a mild suppressive effect. Both extracts could stimulate Th1 and Th2 cytokine mRNA expression, but GL-M was a relatively stronger Th1 stimulator. Different from GL-S, GL-M enhanced maturation of DCs in terms of upregulation of CD40, CD80, and CD86, and also reduced fluorescein isothiocyanate dextran endocytosis. Interestingly, GL-M-treated DCs only modestly enhanced lymphocyte proliferation in allogenic mixed lymphocyte culture with mild enhancement in Th development. Conclusion: These findings provide evidences that GL-M has immunomodulating effects on human immune cells and therefore can be used as a natural adjuvant for cancer immunotherapy with DCs. © Mary Ann Liebert, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/70382
ISSN
2015 Impact Factor: 1.395
2015 SCImago Journal Rankings: 0.475
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, WKen_HK
dc.contributor.authorLam, DTWen_HK
dc.contributor.authorLaw, HKWen_HK
dc.contributor.authorWong, WTen_HK
dc.contributor.authorKoo, MWLen_HK
dc.contributor.authorLau, ASYen_HK
dc.contributor.authorLau, YLen_HK
dc.contributor.authorChan, GCFen_HK
dc.date.accessioned2010-09-06T06:22:21Z-
dc.date.available2010-09-06T06:22:21Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Alternative And Complementary Medicine, 2005, v. 11 n. 6, p. 1047-1057en_HK
dc.identifier.issn1075-5535en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70382-
dc.description.abstractObjectives: Ganoderma lucidum (GL) is one of the most commonly used Chinese herbs in the oriental community, with more than 30% of pediatric cancer patients taking GL. The immunomodulating and anticancer effects exerted by GL extracts have been demonstrated by in vitro and in vivo studies. There was, however, no comparison between the immunomodulating effects of GL mycelium extract (GL-M) and spore extracts on human immune cells. Dendritic cells (DCs) are professional antigen-presenting cells and their role in DC-based tumor vaccine has been well defined. The possibility of GL as natural adjuvant for human DCs remains unknown. Design: This study explored the differential effect of GL-M and GL spore extract (GL-S) on proliferation and Th1/Th2 cytokine mRNA expression of human peripheral blood mononuclear cells (PBMCs) and monocytes. Their effects on the phenotypic and functional maturation of human monocyte-derived DCs were also investigated. Results: GL-M induced the proliferation of PBMCs and monocytes, whereas GL-S showed a mild suppressive effect. Both extracts could stimulate Th1 and Th2 cytokine mRNA expression, but GL-M was a relatively stronger Th1 stimulator. Different from GL-S, GL-M enhanced maturation of DCs in terms of upregulation of CD40, CD80, and CD86, and also reduced fluorescein isothiocyanate dextran endocytosis. Interestingly, GL-M-treated DCs only modestly enhanced lymphocyte proliferation in allogenic mixed lymphocyte culture with mild enhancement in Th development. Conclusion: These findings provide evidences that GL-M has immunomodulating effects on human immune cells and therefore can be used as a natural adjuvant for cancer immunotherapy with DCs. © Mary Ann Liebert, Inc.en_HK
dc.languageengen_HK
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/acmen_HK
dc.relation.ispartofJournal of Alternative and Complementary Medicineen_HK
dc.titleGanoderma lucidum mycelium and spore extracts as natural adjuvants for immunotherapyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1075-5535&volume=11&issue=6&spage=1047&epage=1057&date=2005&atitle=Ganoderma+lucidum+mycelium+and+spore+extracts+as+natural+adjuvants+for+immunotherapyen_HK
dc.identifier.emailWong, WT: wtwong@hku.hken_HK
dc.identifier.emailKoo, MWL: wlkoo@hku.hken_HK
dc.identifier.emailLau, ASY: asylau@hku.hken_HK
dc.identifier.emailLau, YL: lauylung@hku.hken_HK
dc.identifier.emailChan, GCF: gcfchan@hku.hken_HK
dc.identifier.authorityWong, WT=rp00811en_HK
dc.identifier.authorityKoo, MWL=rp00233en_HK
dc.identifier.authorityLau, ASY=rp00474en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.identifier.authorityChan, GCF=rp00431en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1089/acm.2005.11.1047en_HK
dc.identifier.pmid16398597-
dc.identifier.scopuseid_2-s2.0-31144457931en_HK
dc.identifier.hkuros119422en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-31144457931&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1047en_HK
dc.identifier.epage1057en_HK
dc.identifier.isiWOS:000234662000017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, WK=55471357400en_HK
dc.identifier.scopusauthoridLam, DTW=11840131300en_HK
dc.identifier.scopusauthoridLaw, HKW=7101939394en_HK
dc.identifier.scopusauthoridWong, WT=7403973084en_HK
dc.identifier.scopusauthoridKoo, MWL=7004550899en_HK
dc.identifier.scopusauthoridLau, ASY=7202626202en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK
dc.identifier.scopusauthoridChan, GCF=16160154400en_HK

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