File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor

TitleEstablishment and characterization of a new cell line derived from human colorectal laterally spreading tumor
Authors
KeywordsCell line
E-cadherin
Invasion
LST-R1
Issue Date2008
PublisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htm
Citation
World Journal Of Gastroenterology, 2008, v. 14 n. 8, p. 1204-1211 How to Cite?
AbstractAim: To study the molecular mechanism of laterally spreading tumor (LST), a cell line [Laterally Spreading Tumor-Rectum 1 (LST-R1)] was derived and the characteristics of this cell line were investigated. Methods: A new cell line (LST-R1) originated from laterally spreading tumor was established. Properties of the cell line were characterized using scanning and transmission electron microscopy, immunohistochemistry method, cytogenetic analysis and nude mice xenograft experiments. In vitro invasion assay, cDNA microarray and Western blotting were used to compare the difference between the LST-R1 and other colorectal cancer cell lines derived from prudent colon cancer. Results: Our study demonstrated that both epithelial special antigen (ESA) and cytokeratin-20 (CK20) were expressed in LST-R1. The cells presented microvilli and tight junction with large nuclei. The karyotypic analysis showed hyperdiploid features with structural chromosome aberrations. The in vivo tumorigenicity was also demonstrated in nude mice xenograft experiments. The invasion assay suggested this cell line has a higher invasive ability. cDNA microarray and Western blotting show the loss of the expression of E-cadherin in LST-R1 cells. Conclusion: We established and characterized a colorectal cancer cell line, LST-R1 and LST-R1 has an obvious malignant tendency, which maybe partially attributed to the changes of the expression of some adhesion molecules, such as E-cadherin. It is also a versatile tool for exploring the original and progressive mechanisms of laterally spreading tumor and the early colon cancer genesis. © 2008 WJG. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/70376
ISSN
2015 Impact Factor: 2.787
2015 SCImago Journal Rankings: 1.076
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, XYen_HK
dc.contributor.authorLai, ZSen_HK
dc.contributor.authorYeung, CMen_HK
dc.contributor.authorWang, JDen_HK
dc.contributor.authorDeng, Wen_HK
dc.contributor.authorLi, HYen_HK
dc.contributor.authorHan, YJen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorJiang, Ben_HK
dc.contributor.authorLin, MCMen_HK
dc.date.accessioned2010-09-06T06:22:17Z-
dc.date.available2010-09-06T06:22:17Z-
dc.date.issued2008en_HK
dc.identifier.citationWorld Journal Of Gastroenterology, 2008, v. 14 n. 8, p. 1204-1211en_HK
dc.identifier.issn1007-9327en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70376-
dc.description.abstractAim: To study the molecular mechanism of laterally spreading tumor (LST), a cell line [Laterally Spreading Tumor-Rectum 1 (LST-R1)] was derived and the characteristics of this cell line were investigated. Methods: A new cell line (LST-R1) originated from laterally spreading tumor was established. Properties of the cell line were characterized using scanning and transmission electron microscopy, immunohistochemistry method, cytogenetic analysis and nude mice xenograft experiments. In vitro invasion assay, cDNA microarray and Western blotting were used to compare the difference between the LST-R1 and other colorectal cancer cell lines derived from prudent colon cancer. Results: Our study demonstrated that both epithelial special antigen (ESA) and cytokeratin-20 (CK20) were expressed in LST-R1. The cells presented microvilli and tight junction with large nuclei. The karyotypic analysis showed hyperdiploid features with structural chromosome aberrations. The in vivo tumorigenicity was also demonstrated in nude mice xenograft experiments. The invasion assay suggested this cell line has a higher invasive ability. cDNA microarray and Western blotting show the loss of the expression of E-cadherin in LST-R1 cells. Conclusion: We established and characterized a colorectal cancer cell line, LST-R1 and LST-R1 has an obvious malignant tendency, which maybe partially attributed to the changes of the expression of some adhesion molecules, such as E-cadherin. It is also a versatile tool for exploring the original and progressive mechanisms of laterally spreading tumor and the early colon cancer genesis. © 2008 WJG. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htmen_HK
dc.relation.ispartofWorld Journal of Gastroenterologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCell lineen_HK
dc.subjectE-cadherinen_HK
dc.subjectInvasionen_HK
dc.subjectLST-R1en_HK
dc.titleEstablishment and characterization of a new cell line derived from human colorectal laterally spreading tumoren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1007-9327&volume=14&issue=8&spage=1204&epage=1211&date=2008&atitle=Establishment+and+Characterization+of+a+New+Cell+Line+Derived+from+Human+Colorectal+Laterally+Spreading+Tumoren_HK
dc.identifier.emailWang, JD: jidewang@gmail.comen_HK
dc.identifier.emailDeng, W: wdeng@hkucc.hku.hken_HK
dc.identifier.emailLin, MCM: mcllin@hkucc.hku.hken_HK
dc.identifier.authorityWang, JD=rp00491en_HK
dc.identifier.authorityDeng, W=rp01640en_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3748/wjg.14.1204en_HK
dc.identifier.pmid18300345-
dc.identifier.pmcidPMC2690667-
dc.identifier.scopuseid_2-s2.0-40549120171en_HK
dc.identifier.hkuros140795en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40549120171&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume14en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1204en_HK
dc.identifier.epage1211en_HK
dc.identifier.isiWOS:000253599100008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, XY=16044392500en_HK
dc.identifier.scopusauthoridLai, ZS=23972664200en_HK
dc.identifier.scopusauthoridYeung, CM=7201354151en_HK
dc.identifier.scopusauthoridWang, JD=35309087500en_HK
dc.identifier.scopusauthoridDeng, W=7202223673en_HK
dc.identifier.scopusauthoridLi, HY=12752056400en_HK
dc.identifier.scopusauthoridHan, YJ=8575481900en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridJiang, B=34770534200en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats