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Article: Capillary electrophoresis for determination of free and albumin-bound bilirubin and the investigation of drug interaction with bilirubin-bound albumin

TitleCapillary electrophoresis for determination of free and albumin-bound bilirubin and the investigation of drug interaction with bilirubin-bound albumin
Authors
KeywordsAlbumin
Bilirubin
Capillary electrophoresis
Cord blood serum
Residual binding capacity
Issue Date2000
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/journal/10008330/home
Citation
Electrophoresis, 2000, v. 21 n. 2, p. 403-410 How to Cite?
AbstractCapillary electrophoresis (CE) is a promising technique for assessment of free bilirubin and its interaction with albumin, as it requires only a small sample volume and provides a rapid and efficient separation of free bilirubin from its albumin-bound complex in a one-phase system. In order to maintain the equilibrium without dissociation of bilirubin from the albumin/bilirubin complex as in real clinical conditions, the coupling of CE with frontal analysis (FA) was investigated. A very large sample plug was introduced hydrodynamically into the capillary (36 cm length, 50 μm inner diameter) at 15 psi·s to develop the frontal conditions during CE separation. The working conditions for CE/FA separation of bilirubin and albumin were optimized as follows: +20 kV; running buffer, 10 mmol/L phosphate and 1 mmol/L ethylenediaminetetraacetic acid (EDTA), pH 7.4. The working range for bilirubin was found to vary from 5 to 206 μmol/L; precision with relative standard deviation (RSD) <2.0% for n = 3 and detection limit (signal to noise, S/N = 2) was 2 μmol/L. The residual binding capacity of a simulated cord blood serum for bilirubin was 26 mg/100 mL at pH 7.4. Bilirubin was shown to be displaced from albumin when aspirin was added. The free bilirubin concentration was found to increase to clinical significant concentrations from 11.9 to 21.1% when increasing aspirin was added in the range of 5-50 mg/100 mL, respectively. Thus, the investigation of aspirin displacement of bilirubin from albumin is clinically important and the CE/FA method is a suitable procedure for this purpose.
Persistent Identifierhttp://hdl.handle.net/10722/70365
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFung, YS-
dc.contributor.authorSun, DX-
dc.contributor.authorYeung, CY-
dc.date.accessioned2010-09-06T06:22:11Z-
dc.date.available2010-09-06T06:22:11Z-
dc.date.issued2000-
dc.identifier.citationElectrophoresis, 2000, v. 21 n. 2, p. 403-410-
dc.identifier.issn0173-0835-
dc.identifier.urihttp://hdl.handle.net/10722/70365-
dc.description.abstractCapillary electrophoresis (CE) is a promising technique for assessment of free bilirubin and its interaction with albumin, as it requires only a small sample volume and provides a rapid and efficient separation of free bilirubin from its albumin-bound complex in a one-phase system. In order to maintain the equilibrium without dissociation of bilirubin from the albumin/bilirubin complex as in real clinical conditions, the coupling of CE with frontal analysis (FA) was investigated. A very large sample plug was introduced hydrodynamically into the capillary (36 cm length, 50 μm inner diameter) at 15 psi·s to develop the frontal conditions during CE separation. The working conditions for CE/FA separation of bilirubin and albumin were optimized as follows: +20 kV; running buffer, 10 mmol/L phosphate and 1 mmol/L ethylenediaminetetraacetic acid (EDTA), pH 7.4. The working range for bilirubin was found to vary from 5 to 206 μmol/L; precision with relative standard deviation (RSD) <2.0% for n = 3 and detection limit (signal to noise, S/N = 2) was 2 μmol/L. The residual binding capacity of a simulated cord blood serum for bilirubin was 26 mg/100 mL at pH 7.4. Bilirubin was shown to be displaced from albumin when aspirin was added. The free bilirubin concentration was found to increase to clinical significant concentrations from 11.9 to 21.1% when increasing aspirin was added in the range of 5-50 mg/100 mL, respectively. Thus, the investigation of aspirin displacement of bilirubin from albumin is clinically important and the CE/FA method is a suitable procedure for this purpose.en_HK
dc.languageeng-
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/journal/10008330/home-
dc.relation.ispartofElectrophoresis-
dc.subjectAlbuminen_HK
dc.subjectBilirubinen_HK
dc.subjectCapillary electrophoresisen_HK
dc.subjectCord blood serumen_HK
dc.subjectResidual binding capacityen_HK
dc.titleCapillary electrophoresis for determination of free and albumin-bound bilirubin and the investigation of drug interaction with bilirubin-bound albumin-
dc.typeArticle-
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0173-0835&volume=21&spage=403&epage=410&date=2000&atitle=Capillary+electrophoresis+for+determination+of+free+and+albumin-bound+bilirubin+and+the+investigation+of+drug+interaction+with+bilirubin-bound+albuminen_HK
dc.identifier.emailFung, YS: ysfung@hku.hk-
dc.identifier.emailYeung, CY: hrmpycy@hkucc.hku.hk-
dc.identifier.authorityFung, YS=rp00697-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1522-2683(20000101)-
dc.identifier.pmid10675022-
dc.identifier.scopuseid_2-s2.0-0001407982en_HK
dc.identifier.hkuros49676-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0001407982&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume21-
dc.identifier.issue2-
dc.identifier.spage403-
dc.identifier.epage410-
dc.identifier.isiWOS:000085174400028-
dc.publisher.placeGermany-
dc.identifier.scopusauthoridFung, YS=13309754700en_HK
dc.identifier.scopusauthoridSun, DX=36788445000en_HK
dc.identifier.scopusauthoridYeung, CY=7201354144en_HK

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