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Article: Proteomic analysis of EZH2 downstream target proteins in hepatocellular carcinoma

TitleProteomic analysis of EZH2 downstream target proteins in hepatocellular carcinoma
Authors
KeywordsEnhancer of zeste homolog 2
Hepatocellular carcinoma
Target gene
Issue Date2007
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2007, v. 7 n. 17, p. 3097-3104 How to Cite?
AbstractEnhancer of zeste homolog 2 (EZH2) is suggested to be a potential therapeutic target and a diagnostic marker for cancer. Our previous study also showed the critical role of EZH2 in hepatocellular carcinoma (HCC) tumorigenesis. The present study is aimed at revealing the comprehensive downstream pathways of EZH2 by functional proteomic profiling. Lentivirus mediated RNA interference (RNAi) was employed to knockdown EZH2 in HCC cells. The 2-DE was employed to compare the expression profile difference between parental and EZH2-knockdown HCC cells. In total, 28 spots were differentially expressed during EZH2 inhibition. Among all, 18 proteins were identified by PMF with MALDI-TOF MS. Western blotting further validated upregulation of 60S acidic ribosomal protein P0 (L10E), and downregulation of two proteins with EZH2 inhibition: stathmin1 and probable protein disulfide isomerase (PDI) ER-60 precursor (ERp57). Moreover, L10E was downregulated with overexpression of EZH2 in hepatocytes, and L10E reversed the effect of EZH2 on cell proliferation, suggesting it a downstream target of EZH2. The comprehensive and comparative analyses of proteins associated with EZH2 could further our understanding on the downstream signal cascade of EZH2 leading to tumorigenesis. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/70352
ISSN
2015 Impact Factor: 4.079
2015 SCImago Journal Rankings: 1.476
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Yen_HK
dc.contributor.authorLin, MCMen_HK
dc.contributor.authorWang, Hen_HK
dc.contributor.authorChan, CYen_HK
dc.contributor.authorJiang, Len_HK
dc.contributor.authorSai, MNen_HK
dc.contributor.authorYu, Jen_HK
dc.contributor.authorHe, MLen_HK
dc.contributor.authorShaw, PCen_HK
dc.contributor.authorYew, DTen_HK
dc.contributor.authorSung, JJen_HK
dc.contributor.authorKung, HFen_HK
dc.date.accessioned2010-09-06T06:22:04Z-
dc.date.available2010-09-06T06:22:04Z-
dc.date.issued2007en_HK
dc.identifier.citationProteomics, 2007, v. 7 n. 17, p. 3097-3104en_HK
dc.identifier.issn1615-9853en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70352-
dc.description.abstractEnhancer of zeste homolog 2 (EZH2) is suggested to be a potential therapeutic target and a diagnostic marker for cancer. Our previous study also showed the critical role of EZH2 in hepatocellular carcinoma (HCC) tumorigenesis. The present study is aimed at revealing the comprehensive downstream pathways of EZH2 by functional proteomic profiling. Lentivirus mediated RNA interference (RNAi) was employed to knockdown EZH2 in HCC cells. The 2-DE was employed to compare the expression profile difference between parental and EZH2-knockdown HCC cells. In total, 28 spots were differentially expressed during EZH2 inhibition. Among all, 18 proteins were identified by PMF with MALDI-TOF MS. Western blotting further validated upregulation of 60S acidic ribosomal protein P0 (L10E), and downregulation of two proteins with EZH2 inhibition: stathmin1 and probable protein disulfide isomerase (PDI) ER-60 precursor (ERp57). Moreover, L10E was downregulated with overexpression of EZH2 in hepatocytes, and L10E reversed the effect of EZH2 on cell proliferation, suggesting it a downstream target of EZH2. The comprehensive and comparative analyses of proteins associated with EZH2 could further our understanding on the downstream signal cascade of EZH2 leading to tumorigenesis. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_HK
dc.relation.ispartofProteomicsen_HK
dc.subjectEnhancer of zeste homolog 2en_HK
dc.subjectHepatocellular carcinomaen_HK
dc.subjectTarget geneen_HK
dc.titleProteomic analysis of EZH2 downstream target proteins in hepatocellular carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9853&volume=7&issue=17&spage=3097&epage=3104&date=2007&atitle=Proteomic+Analysis+of+EZH2+Downstream+Target+Proteins+in+Hepatocellular+Carcinomaen_HK
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pmic.200700019en_HK
dc.identifier.pmid17676662-
dc.identifier.scopuseid_2-s2.0-34748893835en_HK
dc.identifier.hkuros132772en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34748893835&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue17en_HK
dc.identifier.spage3097en_HK
dc.identifier.epage3104en_HK
dc.identifier.isiWOS:000249635200007-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridChen, Y=24075600300en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK
dc.identifier.scopusauthoridWang, H=8443997400en_HK
dc.identifier.scopusauthoridChan, CY=22033276600en_HK
dc.identifier.scopusauthoridJiang, L=37000894400en_HK
dc.identifier.scopusauthoridSai, MN=22035995700en_HK
dc.identifier.scopusauthoridYu, J=35351306800en_HK
dc.identifier.scopusauthoridHe, ML=35080389700en_HK
dc.identifier.scopusauthoridShaw, PC=35599523600en_HK
dc.identifier.scopusauthoridYew, DT=7007034694en_HK
dc.identifier.scopusauthoridSung, JJ=35405352400en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK

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