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Article: Specific labelling of sulfhydryl-containing biomolecules with redox-active N-(ferrocenyl)iodoacetamide

TitleSpecific labelling of sulfhydryl-containing biomolecules with redox-active N-(ferrocenyl)iodoacetamide
Authors
Issue Date2002
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/dalton
Citation
Journal Of The Chemical Society, Dalton Transactions, 2002 n. 8, p. 1753-1756 How to Cite?
AbstractThe synthesis, characterisation, X-ray crystal structure and electrochemical properties of a new redox-active labelling reagent, N-(ferrocenyl)iodoacetamide (Fc-IAA), are reported. This compound exhibits a reversible ferrocenium/ferrocene couple at ca. +0.345 V vs. SCE at a sweep rate of 100 mV s-1 in CH3CN at 298 K, and two irreversible reduction waves at ca. -1.324 and -2.048 V, attributable to the reduction of the iodoacetamide group. Since the iodoacetamide moiety can react specifically with the sulfhydryl group, Fc-IAA has been coupled to various biomolecules including a sulfhydryl-modified oligonucleotide, cysteine, glutathione and sulfhydryl-modified bovine serum albumin. The electrochemical properties of the bioconjugates have also been investigated.
Persistent Identifierhttp://hdl.handle.net/10722/70335
ISSN
References

 

DC FieldValueLanguage
dc.contributor.authorLo, KKWen_HK
dc.contributor.authorLau, JSYen_HK
dc.contributor.authorNg, DCMen_HK
dc.contributor.authorZhu, Nen_HK
dc.date.accessioned2010-09-06T06:21:54Z-
dc.date.available2010-09-06T06:21:54Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of The Chemical Society, Dalton Transactions, 2002 n. 8, p. 1753-1756en_HK
dc.identifier.issn1470-479Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/70335-
dc.description.abstractThe synthesis, characterisation, X-ray crystal structure and electrochemical properties of a new redox-active labelling reagent, N-(ferrocenyl)iodoacetamide (Fc-IAA), are reported. This compound exhibits a reversible ferrocenium/ferrocene couple at ca. +0.345 V vs. SCE at a sweep rate of 100 mV s-1 in CH3CN at 298 K, and two irreversible reduction waves at ca. -1.324 and -2.048 V, attributable to the reduction of the iodoacetamide group. Since the iodoacetamide moiety can react specifically with the sulfhydryl group, Fc-IAA has been coupled to various biomolecules including a sulfhydryl-modified oligonucleotide, cysteine, glutathione and sulfhydryl-modified bovine serum albumin. The electrochemical properties of the bioconjugates have also been investigated.en_HK
dc.languageengen_HK
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/daltonen_HK
dc.relation.ispartofJournal of the Chemical Society, Dalton Transactionsen_HK
dc.titleSpecific labelling of sulfhydryl-containing biomolecules with redox-active N-(ferrocenyl)iodoacetamideen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1472-7773&volume=&spage=1753&epage=1756&date=2002&atitle=Specific+labelling+of+sulfhydryl-containing+biomolecules+with+redox-active+N-(ferrocenyl)iodoacetamideen_HK
dc.identifier.emailZhu, N: nzhu@hkucc.hku.hken_HK
dc.identifier.authorityZhu, N=rp00845en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0036009981en_HK
dc.identifier.hkuros69074en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036009981&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.issue8en_HK
dc.identifier.spage1753en_HK
dc.identifier.epage1756en_HK
dc.identifier.scopusauthoridLo, KKW=26032181200en_HK
dc.identifier.scopusauthoridLau, JSY=13805764500en_HK
dc.identifier.scopusauthoridNg, DCM=13805259600en_HK
dc.identifier.scopusauthoridZhu, N=7201449530en_HK

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