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Article: The stepwise process of chromium-induced DNA breakage: Characterization by electrochemistry, atomic force microscopy, and DNA electrophoresis

TitleThe stepwise process of chromium-induced DNA breakage: Characterization by electrochemistry, atomic force microscopy, and DNA electrophoresis
Authors
Issue Date2005
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/crt
Citation
Chemical Research In Toxicology, 2005, v. 18 n. 10, p. 1563-1566 How to Cite?
AbstractDNA conformational change and breakage induced by Cr(VI)-GSH interaction were characterized by the integrated tools of electrochemistry, atomic force microscopy (AFM), and DNA electrophoresis. While electrochemistry confirmed the formation of the active species generated from Cr(VI)-GSH reduction, which causes the DNA conformational changes, AFM imaging vividly demonstrated the stepwise process of the DNA denaturation and breakage for the first time. Our DNA electrophoresis further validated that the DNA breakage occurs unevenly at both of the single strands of the molecule. A scheme was drawn based on the experimental observations to explain the phenomenon of the Cr-induced DNA cleavage. © 2005 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/70316
ISSN
2015 Impact Factor: 3.025
2015 SCImago Journal Rankings: 1.323
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, PHen_HK
dc.contributor.authorGao, HYen_HK
dc.contributor.authorCai, Jen_HK
dc.contributor.authorChiu, JFen_HK
dc.contributor.authorSun, Hen_HK
dc.contributor.authorHe, QYen_HK
dc.date.accessioned2010-09-06T06:21:43Z-
dc.date.available2010-09-06T06:21:43Z-
dc.date.issued2005en_HK
dc.identifier.citationChemical Research In Toxicology, 2005, v. 18 n. 10, p. 1563-1566en_HK
dc.identifier.issn0893-228Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/70316-
dc.description.abstractDNA conformational change and breakage induced by Cr(VI)-GSH interaction were characterized by the integrated tools of electrochemistry, atomic force microscopy (AFM), and DNA electrophoresis. While electrochemistry confirmed the formation of the active species generated from Cr(VI)-GSH reduction, which causes the DNA conformational changes, AFM imaging vividly demonstrated the stepwise process of the DNA denaturation and breakage for the first time. Our DNA electrophoresis further validated that the DNA breakage occurs unevenly at both of the single strands of the molecule. A scheme was drawn based on the experimental observations to explain the phenomenon of the Cr-induced DNA cleavage. © 2005 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/crten_HK
dc.relation.ispartofChemical Research in Toxicologyen_HK
dc.titleThe stepwise process of chromium-induced DNA breakage: Characterization by electrochemistry, atomic force microscopy, and DNA electrophoresisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0893-228X&volume=18&spage=1563&epage=1566&date=2005&atitle=The+stepwise+process+of+chromium-induced+DNA+breakage:+characterization+by+electrochemistry,+atomic+force+microscopy,+and+DNA+electrophoresisen_HK
dc.identifier.emailSun, H:hsun@hkucc.hku.hken_HK
dc.identifier.authoritySun, H=rp00777en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/tx050134wen_HK
dc.identifier.pmid16533020-
dc.identifier.scopuseid_2-s2.0-27144451743en_HK
dc.identifier.hkuros120596en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27144451743&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1563en_HK
dc.identifier.epage1566en_HK
dc.identifier.isiWOS:000232740300010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYang, PH=8847694800en_HK
dc.identifier.scopusauthoridGao, HY=40861152800en_HK
dc.identifier.scopusauthoridCai, J=7403153420en_HK
dc.identifier.scopusauthoridChiu, JF=7201501692en_HK
dc.identifier.scopusauthoridSun, H=7404827446en_HK
dc.identifier.scopusauthoridHe, QY=34770287900en_HK

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