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Article: Proteomic identification of the cus system as a major determinant of constitutive escherichia coli silver resistance of chromosomal origin

TitleProteomic identification of the cus system as a major determinant of constitutive escherichia coli silver resistance of chromosomal origin
Authors
KeywordsAntibacterial agents
Bacterial metal resistance
Copper
Efflux pump
Escherichia coli
Silver
Issue Date2008
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jprobs
Citation
Journal Of Proteome Research, 2008, v. 7 n. 6, p. 2351-2356 How to Cite?
AbstractAlthough silver is one of the most potent and rapidly acting toxic metals to bacteria, silver-resistant bacteria do exist with low incidence. A proteomic approach was employed to identify the silver resistance determinants of a silver-resistant Escherichia coli strain isolated from stepwise selection against increasing concentrations of silver (Li et al. J. Bacteriol 1997, 179, 6127-32). Two-dimensional gel electrophoresis and mass spectrometry analysis revealed that members of the CusCFBA copper/silver chemiosmotic efflux system were highly expressed in the silver-resistant strain but undetectable in the parental silver-sensitive strain. Disruption of the cus locus of the silver-resistant strain resulted in a decrease of the minimum inhibitory concentration of Ag + from more than 1 mM to 12 μM. These results suggest that the chromosomally encoded Cus system, which naturally controls the periplasmic copper concentrations, is selectable to confer a constitutive silver resistance phenotype. © 2008 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/70312
ISSN
2015 Impact Factor: 4.173
2015 SCImago Journal Rankings: 1.962
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLok, CNen_HK
dc.contributor.authorHo, CMen_HK
dc.contributor.authorChen, Ren_HK
dc.contributor.authorTam, PKHen_HK
dc.contributor.authorChiu, JFen_HK
dc.contributor.authorChe, CMen_HK
dc.date.accessioned2010-09-06T06:21:41Z-
dc.date.available2010-09-06T06:21:41Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Proteome Research, 2008, v. 7 n. 6, p. 2351-2356en_HK
dc.identifier.issn1535-3893en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70312-
dc.description.abstractAlthough silver is one of the most potent and rapidly acting toxic metals to bacteria, silver-resistant bacteria do exist with low incidence. A proteomic approach was employed to identify the silver resistance determinants of a silver-resistant Escherichia coli strain isolated from stepwise selection against increasing concentrations of silver (Li et al. J. Bacteriol 1997, 179, 6127-32). Two-dimensional gel electrophoresis and mass spectrometry analysis revealed that members of the CusCFBA copper/silver chemiosmotic efflux system were highly expressed in the silver-resistant strain but undetectable in the parental silver-sensitive strain. Disruption of the cus locus of the silver-resistant strain resulted in a decrease of the minimum inhibitory concentration of Ag + from more than 1 mM to 12 μM. These results suggest that the chromosomally encoded Cus system, which naturally controls the periplasmic copper concentrations, is selectable to confer a constitutive silver resistance phenotype. © 2008 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jprobsen_HK
dc.relation.ispartofJournal of Proteome Researchen_HK
dc.subjectAntibacterial agentsen_HK
dc.subjectBacterial metal resistanceen_HK
dc.subjectCopperen_HK
dc.subjectEfflux pumpen_HK
dc.subjectEscherichia colien_HK
dc.subjectSilveren_HK
dc.titleProteomic identification of the cus system as a major determinant of constitutive escherichia coli silver resistance of chromosomal originen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1535-3893&volume=7&spage=2351&epage=2356&date=2008&atitle=Proteomic+identification+of+the+Cus+system+as+a+major+determinant+of+constitutive+escherichia+coli+silver+resistance+of+chromosomal+originen_HK
dc.identifier.emailLok, CN: cnlok@hku.hken_HK
dc.identifier.emailHo, CM: rickyho@hkucc.hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hku.hken_HK
dc.identifier.emailChe, CM: cmche@hku.hken_HK
dc.identifier.authorityLok, CN=rp00752en_HK
dc.identifier.authorityHo, CM=rp00705en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.identifier.authorityChe, CM=rp00670en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/pr700646ben_HK
dc.identifier.scopuseid_2-s2.0-49849084771en_HK
dc.identifier.hkuros143231en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-49849084771&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue6en_HK
dc.identifier.spage2351en_HK
dc.identifier.epage2356en_HK
dc.identifier.isiWOS:000256599000018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLok, CN=7006410829en_HK
dc.identifier.scopusauthoridHo, CM=12807243800en_HK
dc.identifier.scopusauthoridChen, R=9275915200en_HK
dc.identifier.scopusauthoridTam, PKH=7202539421en_HK
dc.identifier.scopusauthoridChiu, JF=7201501692en_HK
dc.identifier.scopusauthoridChe, CM=7102442791en_HK

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