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Article: Bilirubin-human serum albumin interaction monitored by capillary zone electrophoresis

TitleBilirubin-human serum albumin interaction monitored by capillary zone electrophoresis
Authors
Issue Date1999
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0269-3879/
Citation
Biomedical Chromatography, 1999, v. 13 n. 4, p. 267-271 How to Cite?
AbstractCapillary zone electrophoresis was used to monitor the interaction between bilirubin and human serum albumin. Cord blood serum samples were injected directly into an uncoated fused-silica capillary (30 cm x 50 μm i.d.) and separation was accomplished within 4 min without extensive sample pretreatment. The most suitable running buffer to separate free bilirubin from albumin bound bilirubin was found to contain 1.0 mmol/L EDTA, 5% acetonitrile and 15 mmol/L phosphate with pH adjusted to 8.4. Approximately two bilirubin dianions could be bound per human serum albumin molecule in the cord blood serum. The binding constant was estimated to be 1.1 x 105 (L/mol) at 25°C and pH 8.4. The peak area ratio of free bilirubin to total bilirubin can be used to determine the bilirubin binding capacity of cord blood serum for the concentration range of total bilirubin from 204 to 340 μmol/L using 1:5 diluted cord blood seras.
Persistent Identifierhttp://hdl.handle.net/10722/70282
ISSN
2015 Impact Factor: 1.729
2015 SCImago Journal Rankings: 0.572
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ben_HK
dc.contributor.authorFung, Yen_HK
dc.contributor.authorLau, Ken_HK
dc.contributor.authorLin, Ben_HK
dc.date.accessioned2010-09-06T06:21:25Z-
dc.date.available2010-09-06T06:21:25Z-
dc.date.issued1999en_HK
dc.identifier.citationBiomedical Chromatography, 1999, v. 13 n. 4, p. 267-271en_HK
dc.identifier.issn0269-3879en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70282-
dc.description.abstractCapillary zone electrophoresis was used to monitor the interaction between bilirubin and human serum albumin. Cord blood serum samples were injected directly into an uncoated fused-silica capillary (30 cm x 50 μm i.d.) and separation was accomplished within 4 min without extensive sample pretreatment. The most suitable running buffer to separate free bilirubin from albumin bound bilirubin was found to contain 1.0 mmol/L EDTA, 5% acetonitrile and 15 mmol/L phosphate with pH adjusted to 8.4. Approximately two bilirubin dianions could be bound per human serum albumin molecule in the cord blood serum. The binding constant was estimated to be 1.1 x 105 (L/mol) at 25°C and pH 8.4. The peak area ratio of free bilirubin to total bilirubin can be used to determine the bilirubin binding capacity of cord blood serum for the concentration range of total bilirubin from 204 to 340 μmol/L using 1:5 diluted cord blood seras.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0269-3879/en_HK
dc.relation.ispartofBiomedical Chromatographyen_HK
dc.rightsBiomedical Chromatography. Copyright © John Wiley & Sons Ltd.en_HK
dc.titleBilirubin-human serum albumin interaction monitored by capillary zone electrophoresisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0269-3879&volume=13&issue=4&spage=267&epage=271&date=1999&atitle=Bilirubin-human+serum+albumin+interaction+monitored+by+capillary+zone+electrophoresisen_HK
dc.identifier.emailFung, Y:ysfung@hku.hken_HK
dc.identifier.authorityFung, Y=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1099-0801(199906)13:4<267::AID-BMC833>3.0.CO;2-Len_HK
dc.identifier.pmid10416058-
dc.identifier.scopuseid_2-s2.0-0032773037en_HK
dc.identifier.hkuros46537en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032773037&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue4en_HK
dc.identifier.spage267en_HK
dc.identifier.epage271en_HK
dc.identifier.isiWOS:000081341200003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZhang, B=35433277700en_HK
dc.identifier.scopusauthoridFung, Y=13309754700en_HK
dc.identifier.scopusauthoridLau, K=7401559826en_HK
dc.identifier.scopusauthoridLin, B=7403508047en_HK

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