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Article: Piezoelectric crystal for sensing bacteria by immobilizing antibodies on divinylsulphone activated poly-m-aminophenol film

TitlePiezoelectric crystal for sensing bacteria by immobilizing antibodies on divinylsulphone activated poly-m-aminophenol film
Authors
KeywordsBacterium detection
Electrode
Immunosensor
Infrared spectrometry
Intermethod comparison
Issue Date2000
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/talanta
Citation
Talanta, 2000, v. 51 n. 1, p. 151-158 How to Cite?
AbstractA polymer sorbent formed at the surface of gold-plated piezoelectric crystal by anodic oxidation of m-aminophenol was investigated by voltammetric, FTIR techniques for antibody coupling after activation by divinylsulphone. A novel film with increased capacity for immobilizing antibodies was obtained using phloroglucinol to modify poly-m-aminophenol via divinylsulphone. Compared with the dip-coating methods using polyethylenimine and (gamma-aminopropyl)trimethoxysilane, this new technique gave more reproducible results for the immobilization of antibody from sample to sample due to the improved homogeneity and reproducibility of the coating. With present modification method, a piezoelectric immunosensor was developed for the detection of Salmonella enteritidis. A detection limit of 1x10(5) cells ml(-1) and an assay time of 25 min were achieved.
Persistent Identifierhttp://hdl.handle.net/10722/70235
ISSN
2015 Impact Factor: 4.035
2015 SCImago Journal Rankings: 1.233

 

DC FieldValueLanguage
dc.contributor.authorFung, YSen_HK
dc.contributor.authorHui, SHen_HK
dc.contributor.authorZhu, DRen_HK
dc.date.accessioned2010-09-06T06:20:59Z-
dc.date.available2010-09-06T06:20:59Z-
dc.date.issued2000en_HK
dc.identifier.citationTalanta, 2000, v. 51 n. 1, p. 151-158en_HK
dc.identifier.issn0039-9140en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70235-
dc.description.abstractA polymer sorbent formed at the surface of gold-plated piezoelectric crystal by anodic oxidation of m-aminophenol was investigated by voltammetric, FTIR techniques for antibody coupling after activation by divinylsulphone. A novel film with increased capacity for immobilizing antibodies was obtained using phloroglucinol to modify poly-m-aminophenol via divinylsulphone. Compared with the dip-coating methods using polyethylenimine and (gamma-aminopropyl)trimethoxysilane, this new technique gave more reproducible results for the immobilization of antibody from sample to sample due to the improved homogeneity and reproducibility of the coating. With present modification method, a piezoelectric immunosensor was developed for the detection of Salmonella enteritidis. A detection limit of 1x10(5) cells ml(-1) and an assay time of 25 min were achieved.-
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/talantaen_HK
dc.relation.ispartofTalantaen_HK
dc.subjectBacterium detection-
dc.subjectElectrode-
dc.subjectImmunosensor-
dc.subjectInfrared spectrometry-
dc.subjectIntermethod comparison-
dc.titlePiezoelectric crystal for sensing bacteria by immobilizing antibodies on divinylsulphone activated poly-m-aminophenol filmen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0039-9140&volume=51&spage=151&epage=158&date=2000&atitle=Piezoelectric+crystal+for+sensing+bacteria+by+immobilizing+antibodies+on+divinylsulphone+activated+poly-m-aminophenol+filmen_HK
dc.identifier.emailFung, YS: ysfung@hku.hken_HK
dc.identifier.authorityFung, YS=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0039-9140(99)00266-0-
dc.identifier.pmid18967847-
dc.identifier.scopuseid_2-s2.0-0034707729-
dc.identifier.hkuros54395en_HK
dc.identifier.volume51-
dc.identifier.issue1-
dc.identifier.spage151-
dc.identifier.epage158-
dc.publisher.placeNetherlands-

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