Article: Interactions between XIAP associated factor 1 and a nuclear co-activator, CBP, in colon cancer cells

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TitleInteractions between XIAP associated factor 1 and a nuclear co-activator, CBP, in colon cancer cells
AuthorsSun, Y1 2
Qiao, L1
Zou, B1
Xia, HHX1
Gu, Q1
Ma, J1
Lin, MCM1
Zhu, Q2
Zhu, S1
Dai, Y1
Wong, BCY1
KeywordscAMP response element-binding protein
Colon cancer
XIAP-associated factor 1
Issue Date2008
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/DIG
CitationDigestion, 2008, v. 77 n. 2, p. 79-86 [How to Cite?]
DOI: http://dx.doi.org/10.1159/000121441
AbstractBackground/Aims: XIAP-associated factor 1 (XAF1) is a nuclear protein. CBP, the cAMP response element binding protein (CREB)-binding protein, plays an important role as a multifunctional transcriptional co-activator. In this investigation, we aimed to study the putative interaction between XAF1 and CBP in colon cancer cells. Methods: Expressions of XAF1 and CBP were detected by Western blot and RT-PCR. The interaction between XAF1 and CBP was investigated by the glutathione S-transferase (GST) pull-down assay, colocalization and co-immunoprecipitation analysis. Cell proliferation was examined by cell number counting. Results: Both XAF1 and CBP were co-localized in the nuclei of colon cancer cells and they demonstrated a physical interaction, as revealed by GST pull-down assay and co-immunoprecipitation analysis. CBP I peptide (residues 1-1098) was the interacting domain for XAF1 binding. The functional implication of the interaction between XAF1 and CBP was demonstrated by the finding that cell growth inhibition by XAF1 was potentiated by cotransfection with CBP. Furthermore, a reporter assay demonstrated that cotransfection with XAF1 and CBP led to marked reduction in phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA)-stimulated adaptor-related protein complex 1 activity. Conclusions: CBP is a novel binding partner of XAF1, and the interaction between XAF1 and CBP and their functional consequence were mediated by adaptor-related protein complex 1. Copyright © 2008 S. Karger AG.
ISSN0012-2823
2011 Impact Factor: 2.046
2011 SCImago Journal Rankings: 0.157
DOIhttp://dx.doi.org/10.1159/000121441
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorSun, Y
dc.contributor.authorQiao, L
dc.contributor.authorZou, B
dc.contributor.authorXia, HHX
dc.contributor.authorGu, Q
dc.contributor.authorMa, J
dc.contributor.authorLin, MCM
dc.contributor.authorZhu, Q
dc.contributor.authorZhu, S
dc.contributor.authorDai, Y
dc.contributor.authorWong, BCY
dc.date.accessioned2010-09-06T06:20:22Z
dc.date.available2010-09-06T06:20:22Z
dc.date.issued2008
dc.description.abstractBackground/Aims: XIAP-associated factor 1 (XAF1) is a nuclear protein. CBP, the cAMP response element binding protein (CREB)-binding protein, plays an important role as a multifunctional transcriptional co-activator. In this investigation, we aimed to study the putative interaction between XAF1 and CBP in colon cancer cells. Methods: Expressions of XAF1 and CBP were detected by Western blot and RT-PCR. The interaction between XAF1 and CBP was investigated by the glutathione S-transferase (GST) pull-down assay, colocalization and co-immunoprecipitation analysis. Cell proliferation was examined by cell number counting. Results: Both XAF1 and CBP were co-localized in the nuclei of colon cancer cells and they demonstrated a physical interaction, as revealed by GST pull-down assay and co-immunoprecipitation analysis. CBP I peptide (residues 1-1098) was the interacting domain for XAF1 binding. The functional implication of the interaction between XAF1 and CBP was demonstrated by the finding that cell growth inhibition by XAF1 was potentiated by cotransfection with CBP. Furthermore, a reporter assay demonstrated that cotransfection with XAF1 and CBP led to marked reduction in phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA)-stimulated adaptor-related protein complex 1 activity. Conclusions: CBP is a novel binding partner of XAF1, and the interaction between XAF1 and CBP and their functional consequence were mediated by adaptor-related protein complex 1. Copyright © 2008 S. Karger AG.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationDigestion, 2008, v. 77 n. 2, p. 79-86 [How to Cite?]
DOI: http://dx.doi.org/10.1159/000121441
dc.identifier.doihttp://dx.doi.org/10.1159/000121441
dc.identifier.epage86
dc.identifier.hkuros141164
dc.identifier.isiWOS:000254893300003
dc.identifier.issn0012-2823
2011 Impact Factor: 2.046
2011 SCImago Journal Rankings: 0.157
dc.identifier.issue2
dc.identifier.openurl
dc.identifier.pmid18362468
dc.identifier.scopuseid_2-s2.0-42349113243
dc.identifier.spage79
dc.identifier.urihttp://hdl.handle.net/10722/70170
dc.identifier.volume77
dc.languageeng
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/DIG
dc.publisher.placeSwitzerland
dc.relation.ispartofDigestion
dc.relation.referencesReferences in Scopus
dc.rightsDigestion. Copyright © S Karger AG.
dc.subject.meshCREB-Binding Protein - genetics - metabolism
dc.subject.meshCarcinoma - metabolism
dc.subject.meshCell Proliferation
dc.subject.meshColonic Neoplasms - metabolism
dc.subject.meshGene Expression
dc.subject.meshGlutathione Transferase
dc.subject.meshHCT116 Cells
dc.subject.meshHT29 Cells
dc.subject.meshHumans
dc.subject.meshImmunoprecipitation
dc.subject.meshIntracellular Signaling Peptides and Proteins
dc.subject.meshNeoplasm Proteins - genetics - metabolism
dc.subject.meshTranscriptional Activation
dc.subject.meshp300-CBP Transcription Factors - metabolism
dc.subjectcAMP response element-binding protein
dc.subjectColon cancer
dc.subjectXIAP-associated factor 1
dc.titleInteractions between XIAP associated factor 1 and a nuclear co-activator, CBP, in colon cancer cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Shanghai Jiaotong University