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Article: Recombinant adeno-associated virus mediated RNA interference inhibits metastasis of nasopharyngeal cancer cells in vivo and in vitro by suppression of Epstein-Barr virus encoded LMP-1

TitleRecombinant adeno-associated virus mediated RNA interference inhibits metastasis of nasopharyngeal cancer cells in vivo and in vitro by suppression of Epstein-Barr virus encoded LMP-1
Authors
KeywordsLMP-1
Metastasis
Nasopharyngeal carcinoma
rAAV
RNAi
Issue Date2006
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal Of Oncology, 2006, v. 29 n. 3, p. 595-603 How to Cite?
AbstractNasopharyngeal carcinoma (NPC) is a highly metastatic carcinoma characterized by consistent association with Epstein-Barr virus (EBV). Of the EBV-encoded product, latent membrane protein-1 (LMP-1) is considered to be an oncoprotein playing an essential role in cell transformation and metastasis. In this study, we used a recombinant adeno-associated virus type 2 vector (rAAV-2) to deliver small hairpin RNA (shRNA) targeting EBV LMP-1 into the EBV-positive human NPC C666-1 cells and evaluated the effect of long-term suppression of LMP-1 on NPC growth and metastasis in vivo and in vitro. An NPC metastasis nude mouse model with NPC xenograft transplanted in liver was established. The NPC C666-1 cells infected with rAAV-shRNA-LMP-1 or rAAV-EGFP were inoculated in the livers of nude mice. Formation of liver and lung metastasis was evaluated at day 14 after tumor inoculation. Our results demonstrate that rAAV-shRNA-LMP-1 effectively infected C666-1 cells and suppressed LMP-1 expression. Such suppression, in turn, did not significantly inhibit tumor growth, but prevented NPC metastasis in the liver as well as in the lung. Consistent with in vivo data, the in vitro studies in NPC C666-1 cell cultures showed that suppression of LMP-1 by rAAV-shRNA-LMP-1 significantly reduced cell mobility and transmembrane invasion ability. These results demonstrated for the first time that long-term suppression of EBV-encoded LMP-1 in vivo is an effective means for preventing NPC metastasis.
Persistent Identifierhttp://hdl.handle.net/10722/70126
ISSN
2015 Impact Factor: 3.018
2015 SCImago Journal Rankings: 1.270
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Xen_HK
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorLi, CYSen_HK
dc.contributor.authorDing, Yen_HK
dc.contributor.authorChau, Den_HK
dc.contributor.authorLi, Gen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorLin, MCMen_HK
dc.contributor.authorPeng, Yen_HK
dc.date.accessioned2010-09-06T06:19:57Z-
dc.date.available2010-09-06T06:19:57Z-
dc.date.issued2006en_HK
dc.identifier.citationInternational Journal Of Oncology, 2006, v. 29 n. 3, p. 595-603en_HK
dc.identifier.issn1019-6439en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70126-
dc.description.abstractNasopharyngeal carcinoma (NPC) is a highly metastatic carcinoma characterized by consistent association with Epstein-Barr virus (EBV). Of the EBV-encoded product, latent membrane protein-1 (LMP-1) is considered to be an oncoprotein playing an essential role in cell transformation and metastasis. In this study, we used a recombinant adeno-associated virus type 2 vector (rAAV-2) to deliver small hairpin RNA (shRNA) targeting EBV LMP-1 into the EBV-positive human NPC C666-1 cells and evaluated the effect of long-term suppression of LMP-1 on NPC growth and metastasis in vivo and in vitro. An NPC metastasis nude mouse model with NPC xenograft transplanted in liver was established. The NPC C666-1 cells infected with rAAV-shRNA-LMP-1 or rAAV-EGFP were inoculated in the livers of nude mice. Formation of liver and lung metastasis was evaluated at day 14 after tumor inoculation. Our results demonstrate that rAAV-shRNA-LMP-1 effectively infected C666-1 cells and suppressed LMP-1 expression. Such suppression, in turn, did not significantly inhibit tumor growth, but prevented NPC metastasis in the liver as well as in the lung. Consistent with in vivo data, the in vitro studies in NPC C666-1 cell cultures showed that suppression of LMP-1 by rAAV-shRNA-LMP-1 significantly reduced cell mobility and transmembrane invasion ability. These results demonstrated for the first time that long-term suppression of EBV-encoded LMP-1 in vivo is an effective means for preventing NPC metastasis.en_HK
dc.languageengen_HK
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/en_HK
dc.relation.ispartofInternational Journal of Oncologyen_HK
dc.subjectLMP-1en_HK
dc.subjectMetastasisen_HK
dc.subjectNasopharyngeal carcinomaen_HK
dc.subjectrAAVen_HK
dc.subjectRNAien_HK
dc.subject.meshDependovirus - genetics-
dc.subject.meshLiver Neoplasms - prevention and control - secondary - virology-
dc.subject.meshLung Neoplasms - prevention and control - secondary - virology-
dc.subject.meshNasopharyngeal Neoplasms - pathology - prevention and control - virology-
dc.subject.meshRNA Interference-
dc.titleRecombinant adeno-associated virus mediated RNA interference inhibits metastasis of nasopharyngeal cancer cells in vivo and in vitro by suppression of Epstein-Barr virus encoded LMP-1en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1019-6439&volume=29&issue=3&spage=595&epage=603&date=2006&atitle=Recombinant+adeno-associated+virus+mediated+RNA+interference+inhibits+metastasis+of+nasopharyngeal+cancer+cells+in+vivo+and+in+vitro+by+suppression+of+Epstein-Barr+virus+encoded+LMP-1en_HK
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid16865275-
dc.identifier.scopuseid_2-s2.0-33749358055en_HK
dc.identifier.hkuros134724en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33749358055&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue3en_HK
dc.identifier.spage595en_HK
dc.identifier.epage603en_HK
dc.identifier.isiWOS:000239579000009-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridLi, X=36680319400en_HK
dc.identifier.scopusauthoridLiu, X=36486564800en_HK
dc.identifier.scopusauthoridLi, CYS=36680428500en_HK
dc.identifier.scopusauthoridDing, Y=7404137178en_HK
dc.identifier.scopusauthoridChau, D=16315084100en_HK
dc.identifier.scopusauthoridLi, G=36013406300en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK
dc.identifier.scopusauthoridPeng, Y=7403419265en_HK

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