File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2

TitleCapillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2
Authors
KeywordsCapillary zone electrophoresis
Interaction
Interleukin 2
Low molecular weight heparin
Issue Date2005
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpba
Citation
Journal Of Pharmaceutical And Biomedical Analysis, 2005, v. 38 n. 3, p. 408-413 How to Cite?
AbstractA method based on capillary zone electrophoresis (CZE) was used to study the interaction between low molecular weight heparin (LMWH) and interleukin 2 (IL-2). The results showed that the increase of the concentration of LMWH led to the decrease of the peak height and the increase of the peak width of IL-2, but the peak areas were kept constant. The binding constant of IL-2 with LMWH was calculated as 1.2 × 106 M-1 by Scatchard analysis, which is in good agreement with the results found in the references using enzyme-linked immunosorbent assay (ELISA). The results demonstrated that the interaction between IL-2 and LMWH is of fast on-and-off kinetic binding reaction. CZE might be used to study not only slow on-and-off rates interactions, but also fast on-and-off rates ones. The binding constant can be calculated easily, and the method can be applied to study a wide range of heparin-protein interactions. © 2005 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/70103
ISSN
2015 Impact Factor: 3.169
2015 SCImago Journal Rankings: 1.049
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiang, Aen_HK
dc.contributor.authorHe, Xen_HK
dc.contributor.authorDu, Yen_HK
dc.contributor.authorWang, Ken_HK
dc.contributor.authorFung, Yen_HK
dc.contributor.authorLin, Ben_HK
dc.date.accessioned2010-09-06T06:19:45Z-
dc.date.available2010-09-06T06:19:45Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Pharmaceutical And Biomedical Analysis, 2005, v. 38 n. 3, p. 408-413en_HK
dc.identifier.issn0731-7085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70103-
dc.description.abstractA method based on capillary zone electrophoresis (CZE) was used to study the interaction between low molecular weight heparin (LMWH) and interleukin 2 (IL-2). The results showed that the increase of the concentration of LMWH led to the decrease of the peak height and the increase of the peak width of IL-2, but the peak areas were kept constant. The binding constant of IL-2 with LMWH was calculated as 1.2 × 106 M-1 by Scatchard analysis, which is in good agreement with the results found in the references using enzyme-linked immunosorbent assay (ELISA). The results demonstrated that the interaction between IL-2 and LMWH is of fast on-and-off kinetic binding reaction. CZE might be used to study not only slow on-and-off rates interactions, but also fast on-and-off rates ones. The binding constant can be calculated easily, and the method can be applied to study a wide range of heparin-protein interactions. © 2005 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jpbaen_HK
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysisen_HK
dc.rightsJournal of Pharmaceutical and Biomedical Analysis. Copyright © Elsevier BV.en_HK
dc.subjectCapillary zone electrophoresisen_HK
dc.subjectInteractionen_HK
dc.subjectInterleukin 2en_HK
dc.subjectLow molecular weight heparinen_HK
dc.titleCapillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0731-7085&volume=38&spage=408&epage=413&date=2005&atitle=Capillary+zone+electrophoresis+characterization+of+low+molecular+weight+heparin+binding+to+interleukin+2en_HK
dc.identifier.emailFung, Y:ysfung@hku.hken_HK
dc.identifier.authorityFung, Y=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jpba.2005.01.019en_HK
dc.identifier.pmid15925240-
dc.identifier.scopuseid_2-s2.0-19544365448en_HK
dc.identifier.hkuros111366en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-19544365448&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue3en_HK
dc.identifier.spage408en_HK
dc.identifier.epage413en_HK
dc.identifier.isiWOS:000230235200004-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLiang, A=7103265241en_HK
dc.identifier.scopusauthoridHe, X=7404408765en_HK
dc.identifier.scopusauthoridDu, Y=7402894093en_HK
dc.identifier.scopusauthoridWang, K=7501400830en_HK
dc.identifier.scopusauthoridFung, Y=13309754700en_HK
dc.identifier.scopusauthoridLin, B=34668182800en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats