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Article: Inhibition of EBV-encoded LMP-1 by DNA-based RNA interference affects metastatic ability of nasopharyngeal carcinoma cells

TitleInhibition of EBV-encoded LMP-1 by DNA-based RNA interference affects metastatic ability of nasopharyngeal carcinoma cells
DNA載體介導RNA干擾抑制LMP-1基因對鼻咽癌細胞轉移能力的影響
Authors
KeywordsDNA-vecto (DNA載體)
RNA interference (RNA干擾)
LMP-1 (LMP-1基因)
Neoplasm metastasis (鼻咽癌)
Nasopharyngeal neoplasms (腫瘤細胞轉移)
Issue Date2006
PublisherCancer Institute and Hospital, Chinese Academy of Medical Sciences (中國醫學科學院中國協和醫科大學腫瘤硏究所腫瘤醫院).
Citation
Chinese Journal of Oncology, 2006, v. 28 n. 10, p. 724-727 How to Cite?
中華腫瘤雜誌, 2006, v. 28 n. 10, p. 724-727 How to Cite?
AbstractOBJECTIVE: To evaluate the application of DNA vector-based RNA interference for silencing LMP-1 expression in EBV-positive nasopharyngeal cancer (NPC) C611 cells and analyze the inhibiting effects of LMP-1 silencing on metastatic property of NPC cells. METHODS: DNA vectors with LMP-1 shRNA template were transfected into C611 cells. After G418 selection, a LMP-1 silenced sub-clone named C611L was isolated. Effect of LMP-1 inhibition on its metastatic properties was evaluated by cell adhesion, invasion and wound healing assays. RESULTS: shRNA vector mediated stable RNAi against LMP-1 in C611 cells. Loss of LMP-1 significantly altered cell motility, substratum adhesion and transmembrane invasion ability. CONCLUSION: Stable suppression of LMP-1 induced by shRNA vector can inhibit metastatic properties of NPC cells.
目的探討應用DNA載體介導RNA干擾技術,抑制鼻咽癌細胞中EB病毒潛伏膜蛋白1(LMP-1)表達的可能性,觀察LMP-1基因沉默對鼻咽癌細胞轉移能力的影響。方法將能轉錄出干擾RNA的DNA模板插入pAVU6+27質粒,構建能夠介導RNA干擾的發夾狀干擾RNA (shRNA)表達載體。采用磷酸鈣共沉淀法,將質粒導入EB病毒(+)的鼻咽癌細胞C611,篩選LMP-1基因沉默的克隆,通過觀察其貼壁生長能力、基底膜穿透能力和移動能力的變化,分析LMP-1基因沉默對鼻咽癌細胞轉移能力的影響。結果在shRNA表達載體有效轉染的C611細胞中,LMP-1基因的表達受到穩定抑制。腫瘤細胞轉移實驗表明,LMP-1基因沉默的C611細胞,貼壁生長能力提高,基底膜穿透能力和細胞移動能力均明顯下降。結論shRNA表達載體能夠在鼻咽癌細胞內介導RNA干擾,并獲得對LMP-1基因穩定的抑制。LMP-1基因可能通過貼壁生長能力、基底膜穿透能力和細胞移動能力等,影響鼻咽癌細胞的轉移。
Persistent Identifierhttp://hdl.handle.net/10722/70032
ISSN
2015 SCImago Journal Rankings: 0.190

 

DC FieldValueLanguage
dc.contributor.authorLi, Gen_HK
dc.contributor.authorLi, XPen_HK
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorPeng, Yen_HK
dc.contributor.authorLin, MCen_HK
dc.date.accessioned2010-09-06T06:19:04Z-
dc.date.available2010-09-06T06:19:04Z-
dc.date.issued2006en_HK
dc.identifier.citationChinese Journal of Oncology, 2006, v. 28 n. 10, p. 724-727en_HK
dc.identifier.citation中華腫瘤雜誌, 2006, v. 28 n. 10, p. 724-727-
dc.identifier.issn0253-3766en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70032-
dc.description.abstractOBJECTIVE: To evaluate the application of DNA vector-based RNA interference for silencing LMP-1 expression in EBV-positive nasopharyngeal cancer (NPC) C611 cells and analyze the inhibiting effects of LMP-1 silencing on metastatic property of NPC cells. METHODS: DNA vectors with LMP-1 shRNA template were transfected into C611 cells. After G418 selection, a LMP-1 silenced sub-clone named C611L was isolated. Effect of LMP-1 inhibition on its metastatic properties was evaluated by cell adhesion, invasion and wound healing assays. RESULTS: shRNA vector mediated stable RNAi against LMP-1 in C611 cells. Loss of LMP-1 significantly altered cell motility, substratum adhesion and transmembrane invasion ability. CONCLUSION: Stable suppression of LMP-1 induced by shRNA vector can inhibit metastatic properties of NPC cells.en_HK
dc.description.abstract目的探討應用DNA載體介導RNA干擾技術,抑制鼻咽癌細胞中EB病毒潛伏膜蛋白1(LMP-1)表達的可能性,觀察LMP-1基因沉默對鼻咽癌細胞轉移能力的影響。方法將能轉錄出干擾RNA的DNA模板插入pAVU6+27質粒,構建能夠介導RNA干擾的發夾狀干擾RNA (shRNA)表達載體。采用磷酸鈣共沉淀法,將質粒導入EB病毒(+)的鼻咽癌細胞C611,篩選LMP-1基因沉默的克隆,通過觀察其貼壁生長能力、基底膜穿透能力和移動能力的變化,分析LMP-1基因沉默對鼻咽癌細胞轉移能力的影響。結果在shRNA表達載體有效轉染的C611細胞中,LMP-1基因的表達受到穩定抑制。腫瘤細胞轉移實驗表明,LMP-1基因沉默的C611細胞,貼壁生長能力提高,基底膜穿透能力和細胞移動能力均明顯下降。結論shRNA表達載體能夠在鼻咽癌細胞內介導RNA干擾,并獲得對LMP-1基因穩定的抑制。LMP-1基因可能通過貼壁生長能力、基底膜穿透能力和細胞移動能力等,影響鼻咽癌細胞的轉移。-
dc.languagechien_HK
dc.publisherCancer Institute and Hospital, Chinese Academy of Medical Sciences (中國醫學科學院中國協和醫科大學腫瘤硏究所腫瘤醫院).-
dc.relation.ispartofChinese Journal of Oncologyen_HK
dc.relation.ispartof中華腫瘤雜誌-
dc.subjectDNA-vecto (DNA載體)-
dc.subjectRNA interference (RNA干擾)-
dc.subjectLMP-1 (LMP-1基因)-
dc.subjectNeoplasm metastasis (鼻咽癌)-
dc.subjectNasopharyngeal neoplasms (腫瘤細胞轉移)-
dc.subject.meshCadherins - metabolism-
dc.subject.meshViral Matrix Proteins - genetics - physiology-
dc.subject.meshRNA, Small Interfering - genetics-
dc.subject.meshRNA Interference-
dc.subject.meshHerpesvirus 4, Human - genetics - growth & development-
dc.titleInhibition of EBV-encoded LMP-1 by DNA-based RNA interference affects metastatic ability of nasopharyngeal carcinoma cellsen_HK
dc.titleDNA載體介導RNA干擾抑制LMP-1基因對鼻咽癌細胞轉移能力的影響-
dc.typeArticleen_HK
dc.identifier.emailLin, MC: mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid17366780-
dc.identifier.scopuseid_2-s2.0-39049092330en_HK
dc.identifier.hkuros134643en_HK
dc.identifier.volume28en_HK
dc.identifier.issue10en_HK
dc.identifier.spage724en_HK
dc.identifier.epage727en_HK
dc.publisher.placePeking-
dc.identifier.scopusauthoridLin, MC=7404816359en_HK
dc.identifier.scopusauthoridPeng, Y=7403419265en_HK
dc.identifier.scopusauthoridLiu, X=35303002700en_HK
dc.identifier.scopusauthoridLi, XP=26663939600en_HK
dc.identifier.scopusauthoridLi, G=36013406300en_HK
dc.customcontrol.immutablecsl 130813-

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