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Article: Ethanol extract of Fructus Ligustri Lucidi promotes osteogenesis of mesenchymal stem cells
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TitleEthanol extract of Fructus Ligustri Lucidi promotes osteogenesis of mesenchymal stem cells
 
AuthorsLi, G2
Zhang, XA2
Zhang, JF2
Chan, CY2 2
Yew, DTW2
He, ML2
Lin, MCM1
Leung, PC2
Kung, HF2 2
 
KeywordsBone marrow
Fructus ligustri lucidi
Mesenchymal stem cells
Osteogenesis
 
Issue Date2010
 
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/12567
 
CitationPhytotherapy Research, 2010, v. 24 n. 4, p. 571-576 [How to Cite?]
DOI: http://dx.doi.org/10.1002/ptr.2987
 
AbstractFructus Ligustri Lucidi (FLL) has been used in traditional Chinese medicine for over 1000 years. The ethanol extract of FLL (EFLL) has been shown to be a potential candidate in the prevention and treatment of osteoporosis. The present study aimed to determine whether EFLL carries out the effect by promoting osteogenesis in mesenchymal stem cells (MSCs). The osteogenic differentiation of MSCs was evaluated by their alkaline phosphatase (ALP) activities and mineralization. Expression of genes was detected by RT-PCR. We found that EFLL significantly stimulated the ALP activities and shortened the time needed for the mineralization of MSCs during osteogenic differentiation. The expression of several osteoblast differentiation regulators was also upregulated by EFLL during this process. Our study demonstrated that the EFLL is capable of enhancing osteogenic differentiation of MSCs. It might be useful for treating diseases with inadequate bone formation, including osteoporosis. Copyright © 2009 John Wiley & Sons, Ltd.
 
ISSN0951-418X
2012 Impact Factor: 2.068
2012 SCImago Journal Rankings: 0.718
 
DOIhttp://dx.doi.org/10.1002/ptr.2987
 
ISI Accession Number IDWOS:000276802500017
Funding AgencyGrant Number
Li Ka Shing Institute of Health Sciences
RGCCUHK 7422_03M
Funding Information:

The authors would like to thank bone marrow donors. We would also thank Dr David Wilmshurst for editing this paper. This work was supported by the Li Ka Shing Institute of Health Sciences Grant and RGC project: CUHK 7422_03M.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLi, G
 
dc.contributor.authorZhang, XA
 
dc.contributor.authorZhang, JF
 
dc.contributor.authorChan, CY
 
dc.contributor.authorYew, DTW
 
dc.contributor.authorHe, ML
 
dc.contributor.authorLin, MCM
 
dc.contributor.authorLeung, PC
 
dc.contributor.authorKung, HF
 
dc.date.accessioned2010-09-06T06:18:10Z
 
dc.date.available2010-09-06T06:18:10Z
 
dc.date.issued2010
 
dc.description.abstractFructus Ligustri Lucidi (FLL) has been used in traditional Chinese medicine for over 1000 years. The ethanol extract of FLL (EFLL) has been shown to be a potential candidate in the prevention and treatment of osteoporosis. The present study aimed to determine whether EFLL carries out the effect by promoting osteogenesis in mesenchymal stem cells (MSCs). The osteogenic differentiation of MSCs was evaluated by their alkaline phosphatase (ALP) activities and mineralization. Expression of genes was detected by RT-PCR. We found that EFLL significantly stimulated the ALP activities and shortened the time needed for the mineralization of MSCs during osteogenic differentiation. The expression of several osteoblast differentiation regulators was also upregulated by EFLL during this process. Our study demonstrated that the EFLL is capable of enhancing osteogenic differentiation of MSCs. It might be useful for treating diseases with inadequate bone formation, including osteoporosis. Copyright © 2009 John Wiley & Sons, Ltd.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationPhytotherapy Research, 2010, v. 24 n. 4, p. 571-576 [How to Cite?]
DOI: http://dx.doi.org/10.1002/ptr.2987
 
dc.identifier.doihttp://dx.doi.org/10.1002/ptr.2987
 
dc.identifier.epage576
 
dc.identifier.hkuros167697
 
dc.identifier.isiWOS:000276802500017
Funding AgencyGrant Number
Li Ka Shing Institute of Health Sciences
RGCCUHK 7422_03M
Funding Information:

The authors would like to thank bone marrow donors. We would also thank Dr David Wilmshurst for editing this paper. This work was supported by the Li Ka Shing Institute of Health Sciences Grant and RGC project: CUHK 7422_03M.

 
dc.identifier.issn0951-418X
2012 Impact Factor: 2.068
2012 SCImago Journal Rankings: 0.718
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid19813230
 
dc.identifier.scopuseid_2-s2.0-77951542282
 
dc.identifier.spage571
 
dc.identifier.urihttp://hdl.handle.net/10722/69932
 
dc.identifier.volume24
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/12567
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofPhytotherapy Research
 
dc.relation.referencesReferences in Scopus
 
dc.rightsPhytotherapy Research. Copyright © John Wiley & Sons Ltd.
 
dc.rightsThe definitive version is available at www.3.interscience.wiley.com
 
dc.subject.meshBone Density Conservation Agents - analysis
 
dc.subject.meshLigustrum - chemistry
 
dc.subject.meshMesenchymal Stem Cells - drug effects - metabolism
 
dc.subject.meshOsteogenesis - drug effects
 
dc.subject.meshPlant Extracts - pharmacology
 
dc.subjectBone marrow
 
dc.subjectFructus ligustri lucidi
 
dc.subjectMesenchymal stem cells
 
dc.subjectOsteogenesis
 
dc.titleEthanol extract of Fructus Ligustri Lucidi promotes osteogenesis of mesenchymal stem cells
 
dc.typeArticle
 
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<contributor.author>He, ML</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Chinese University of Hong Kong