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Article: A sheath-flow nanoelectrospray interface of microchip electrophoresis MS for glycoprotein and glycopeptide analysis

TitleA sheath-flow nanoelectrospray interface of microchip electrophoresis MS for glycoprotein and glycopeptide analysis
Authors
KeywordsGlycopeptides
Glycoprotein
Microchip electrophoresis
MS
Nanoelectrospray interface
Issue Date2006
PublisherWiley - V C H Verlag GmbH & Co KGaA.
Citation
Electrophoresis, 2006, v. 27 n. 24, p. 5059-5067 How to Cite?
AbstractMicrochip was coupled with MS through a stable, sensitive, and controllable sheath-flow nanoelectrospray (nES) interface for glycoprotein and glycopeptide analysis. The nano-ESI (nESI) was made with a delivery capillary, a commercial nES capillary, and a stainless steel (SS) tube which were connected together through a tee unit. High voltage for nES was applied on the SS tube and the commercial nES capillary was used as nES emitter. The delivery capillary was attached to the microchannel for delivering liquid from microchip to the nESI source. The flow rate of sheath liquid was optimized to be 100-200 nL/ min which largely reduced the sample dilution. The detection limit of peptides on this microchip/MS platform was at femtomole level. Glycoprotein and glycopeptides were also successfully analyzed on the platform. All the glycoforms and glycopeptides of ribonuclease B (RNase B) were identified with this method. Some structures of the glycopeptides from RNase B were further characterized with MS/MS on the microchip, coupled with a quadrupole IT-MS. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Persistent Identifierhttp://hdl.handle.net/10722/69919
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMao, Xen_HK
dc.contributor.authorChu, IKen_HK
dc.contributor.authorLin, Ben_HK
dc.date.accessioned2010-09-06T06:18:02Z-
dc.date.available2010-09-06T06:18:02Z-
dc.date.issued2006en_HK
dc.identifier.citationElectrophoresis, 2006, v. 27 n. 24, p. 5059-5067en_HK
dc.identifier.issn0173-0835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69919-
dc.description.abstractMicrochip was coupled with MS through a stable, sensitive, and controllable sheath-flow nanoelectrospray (nES) interface for glycoprotein and glycopeptide analysis. The nano-ESI (nESI) was made with a delivery capillary, a commercial nES capillary, and a stainless steel (SS) tube which were connected together through a tee unit. High voltage for nES was applied on the SS tube and the commercial nES capillary was used as nES emitter. The delivery capillary was attached to the microchannel for delivering liquid from microchip to the nESI source. The flow rate of sheath liquid was optimized to be 100-200 nL/ min which largely reduced the sample dilution. The detection limit of peptides on this microchip/MS platform was at femtomole level. Glycoprotein and glycopeptides were also successfully analyzed on the platform. All the glycoforms and glycopeptides of ribonuclease B (RNase B) were identified with this method. Some structures of the glycopeptides from RNase B were further characterized with MS/MS on the microchip, coupled with a quadrupole IT-MS. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA.en_HK
dc.relation.ispartofElectrophoresisen_HK
dc.subjectGlycopeptidesen_HK
dc.subjectGlycoproteinen_HK
dc.subjectMicrochip electrophoresisen_HK
dc.subjectMSen_HK
dc.subjectNanoelectrospray interfaceen_HK
dc.titleA sheath-flow nanoelectrospray interface of microchip electrophoresis MS for glycoprotein and glycopeptide analysisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0173-0835&volume=27&spage=5059&epage=5067&date=2006&atitle=A+sheath-flow+nanoelectrospray+interface+of+microchip+electrophoresis+MS+for+glycoprotein+and+glycopeptide+analysisen_HK
dc.identifier.emailChu, IK:ivankchu@hku.hken_HK
dc.identifier.authorityChu, IK=rp00683en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/elps.200600349en_HK
dc.identifier.pmid17117389en_HK
dc.identifier.scopuseid_2-s2.0-33846031560en_HK
dc.identifier.hkuros125257en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846031560&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume27en_HK
dc.identifier.issue24en_HK
dc.identifier.spage5059en_HK
dc.identifier.epage5067en_HK
dc.identifier.isiWOS:000243304100022-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridMao, X=7402841292en_HK
dc.identifier.scopusauthoridChu, IK=7103327484en_HK
dc.identifier.scopusauthoridLin, B=34668182800en_HK

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