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Article: A collision model for DNA separation by capillary electrophoresis in dilute polymer solution

TitleA collision model for DNA separation by capillary electrophoresis in dilute polymer solution
Authors
Issue Date2001
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm
Citation
Analytical And Bioanalytical Chemistry, 2001, v. 370 n. 8, p. 1015-1022 How to Cite?
AbstractA theoretical description, based on chemical kinetics and electrochemistry, is given of DNA separation in dilute polymer solution by capillary electrophoresis. A self-consistent model was developed leading to predictions of the DNA electrophoretic velocity as a function of the experimental conditions - polymer concentration, temperature, and electric field strength. The effect of selected experimental variables is discussed. The phenomena discussed are illustrated for the example of 100 bp DNA ladder separation in dilute HPMC solution by capillary electrophoresis. This model is the first single model that can fully explain the dependence of DNA electrophoretic velocity on electrophoretic conditions. © Springer-Verlag 2001.
Persistent Identifierhttp://hdl.handle.net/10722/69915
ISSN
2015 Impact Factor: 3.125
2015 SCImago Journal Rankings: 1.096
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, Yen_HK
dc.contributor.authorLin, BCen_HK
dc.contributor.authorFung, YSen_HK
dc.date.accessioned2010-09-06T06:18:00Z-
dc.date.available2010-09-06T06:18:00Z-
dc.date.issued2001en_HK
dc.identifier.citationAnalytical And Bioanalytical Chemistry, 2001, v. 370 n. 8, p. 1015-1022en_HK
dc.identifier.issn1618-2642en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69915-
dc.description.abstractA theoretical description, based on chemical kinetics and electrochemistry, is given of DNA separation in dilute polymer solution by capillary electrophoresis. A self-consistent model was developed leading to predictions of the DNA electrophoretic velocity as a function of the experimental conditions - polymer concentration, temperature, and electric field strength. The effect of selected experimental variables is discussed. The phenomena discussed are illustrated for the example of 100 bp DNA ladder separation in dilute HPMC solution by capillary electrophoresis. This model is the first single model that can fully explain the dependence of DNA electrophoretic velocity on electrophoretic conditions. © Springer-Verlag 2001.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htmen_HK
dc.relation.ispartofAnalytical and Bioanalytical Chemistryen_HK
dc.titleA collision model for DNA separation by capillary electrophoresis in dilute polymer solutionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0937-0633&volume=370&spage=1015&epage=1022&date=2001&atitle=A+collision+model+for+DNA+separation+by+capillary+electrophoresis+in+dilute+polymer+solutionen_HK
dc.identifier.emailFung, YS:ysfung@hku.hken_HK
dc.identifier.authorityFung, YS=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s002160100902-
dc.identifier.pmid11583080-
dc.identifier.scopuseid_2-s2.0-0035724355en_HK
dc.identifier.hkuros68492en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035724355&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume370en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1015en_HK
dc.identifier.epage1022en_HK
dc.identifier.isiWOS:000175276300007-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridJin, Y=39861604500en_HK
dc.identifier.scopusauthoridLin, BC=7403508047en_HK
dc.identifier.scopusauthoridFung, YS=13309754700en_HK

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