File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection

TitleRapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection
Authors
KeywordsElectrophoresis
Ginseng
Microchip
Molecular authentication
Short tandem repeats (STR)
Issue Date2005
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm
Citation
Analytical And Bioanalytical Chemistry, 2005, v. 381 n. 4, p. 812-819 How to Cite?
AbstractGinseng is one of the most expensive Chinese herbal medicines and the effectiveness of ginseng depends strongly on its botanical sources and the use of different parts of the plants. In this study, a microchip electrophoresis method coupled with the polymerase chain reaction (PCR)-short tandem repeats (STR) technique was developed for rapid authentication of ginseng species. A low viscosity hydroxypropyl methylcellulose (HPMC) solution was used as the sieving matrix for separation of the amplified STR fragments. The allele sizing of the amplified PCR products could be detected within 240 s or less. Good reproducibility and accuracy of the fragment size were obtained with the relative standard deviation for the allele sizes less than 1.0% (n=11). At two microsatellite loci (CT 12, CA 33), American ginseng had a different allele pattern on the electropherograms compared with that of the Oriental ginseng. Moreover, cultivated and wild American ginseng can be distinguished on the basis of allele sizing. This work establishes the feasibility of fast genetic authentication of ginseng species by use of microchip electrophoresis. © Springer-Verlag 2004.
Persistent Identifierhttp://hdl.handle.net/10722/69812
ISSN
2015 Impact Factor: 3.125
2015 SCImago Journal Rankings: 1.096
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQin, Jen_HK
dc.contributor.authorLeung, FCen_HK
dc.contributor.authorFung, Yen_HK
dc.contributor.authorZhu, Den_HK
dc.contributor.authorLin, Ben_HK
dc.date.accessioned2010-09-06T06:17:04Z-
dc.date.available2010-09-06T06:17:04Z-
dc.date.issued2005en_HK
dc.identifier.citationAnalytical And Bioanalytical Chemistry, 2005, v. 381 n. 4, p. 812-819en_HK
dc.identifier.issn1618-2642en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69812-
dc.description.abstractGinseng is one of the most expensive Chinese herbal medicines and the effectiveness of ginseng depends strongly on its botanical sources and the use of different parts of the plants. In this study, a microchip electrophoresis method coupled with the polymerase chain reaction (PCR)-short tandem repeats (STR) technique was developed for rapid authentication of ginseng species. A low viscosity hydroxypropyl methylcellulose (HPMC) solution was used as the sieving matrix for separation of the amplified STR fragments. The allele sizing of the amplified PCR products could be detected within 240 s or less. Good reproducibility and accuracy of the fragment size were obtained with the relative standard deviation for the allele sizes less than 1.0% (n=11). At two microsatellite loci (CT 12, CA 33), American ginseng had a different allele pattern on the electropherograms compared with that of the Oriental ginseng. Moreover, cultivated and wild American ginseng can be distinguished on the basis of allele sizing. This work establishes the feasibility of fast genetic authentication of ginseng species by use of microchip electrophoresis. © Springer-Verlag 2004.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htmen_HK
dc.relation.ispartofAnalytical and Bioanalytical Chemistryen_HK
dc.subjectElectrophoresisen_HK
dc.subjectGinsengen_HK
dc.subjectMicrochipen_HK
dc.subjectMolecular authenticationen_HK
dc.subjectShort tandem repeats (STR)en_HK
dc.titleRapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1618-2642&volume=381&spage=812&epage=819&date=2005&atitle=Rapid+authentication+of+ginseng+species+using+microchip+electrophoresis+with+laser-induced+fluorescence+detectionen_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.emailFung, Y: ysfung@hku.hken_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.identifier.authorityFung, Y=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00216-004-2889-2en_HK
dc.identifier.pmid15750870-
dc.identifier.scopuseid_2-s2.0-16444376511en_HK
dc.identifier.hkuros104856en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-16444376511&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume381en_HK
dc.identifier.issue4en_HK
dc.identifier.spage812en_HK
dc.identifier.epage819en_HK
dc.identifier.isiWOS:000227450200003-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridQin, J=7402895572en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK
dc.identifier.scopusauthoridFung, Y=13309754700en_HK
dc.identifier.scopusauthoridZhu, D=7403599128en_HK
dc.identifier.scopusauthoridLin, B=34668182800en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats