File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Revisit the complexation of PEI and DNA - How to make low cytotoxic and highly efficient PEI gene transfection non-viral vectors with a controllable chain length and structure?

TitleRevisit the complexation of PEI and DNA - How to make low cytotoxic and highly efficient PEI gene transfection non-viral vectors with a controllable chain length and structure?
Authors
KeywordsChain structure
Disulfide
Gene delivery
Laser light scattering
Polyethyleneimine
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrel
Citation
Journal Of Controlled Release, 2009, v. 140 n. 1, p. 40-46 How to Cite?
AbstractThe commercially available branched polyethyleneimine (PEI) with a molar mass of 25 kD (PEI-25K) is an effective in vitro vector to transfer genes, but its cytotoxicity limits its applications in bio-related research. To solve such an efficiency-versus-cytotoxicity catch-22 problem, the disulfide bond has been previously used to link less toxic short PEI chains (2 kD), but previous literature results are controversial. Recently, we found that it is vitally important to remove both carbon dioxide and water in the linking reaction as well as to control the structure of the resultant chains linked by dithiobis(succinimidyl propionate) (DSP). Under a programmable mixing of PEI and DSP, we can use laser light scattering (LLS) to in-situ monitor the linking reaction kinetics in DMSO in terms of the change of the average molar mass (Mw). Therefore, we were able to withdraw a series of linked PEI chains with different molar masses from one reaction mixture. Two such linked PEI samples (Mw ~ 7 kD, PEI-7K-L and ~ 400 kD, PEI-400K-L) were used to illustrate the effect of the sample preparation and the chain structure on the in vitro gene transfection and cytotoxicity. Our results reveal that PEI-7K-L is less cytotoxic and more effective in the gene transfection than both PEI-25K and Lipofectamine 2000 in the in vitro gene transfection. However, PEI-400K-L has no gene transfection efficiency even though it is non-toxic. © 2009 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/69597
ISSN
2015 Impact Factor: 7.441
2015 SCImago Journal Rankings: 2.827
ISI Accession Number ID
Funding AgencyGrant Number
Chinese Academy of Sciences (CAS)KJCX2-SW-H14
National Natural Scientific Foundation of China (NNSFC)20534020
20574065
Hong Kong Special Administration Region (HKSAR)CUHK4036/05P
2160269
Funding Information:

The financial support of the Chinese Academy of Sciences (CAS) Special Grant (KJCX2-SW-H14), the National Natural Scientific Foundation of China (NNSFC) Projects (20534020 and 20574065) and the Hong Kong Special Administration Region (HKSAR) Earmarked Project (CUHK4036/05P, 2160269) is gratefully acknowledged.

References

 

DC FieldValueLanguage
dc.contributor.authorDeng, Ren_HK
dc.contributor.authorYue, Yen_HK
dc.contributor.authorJin, Fen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorLin, MCMen_HK
dc.contributor.authorWu, Cen_HK
dc.date.accessioned2010-09-06T06:15:07Z-
dc.date.available2010-09-06T06:15:07Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Controlled Release, 2009, v. 140 n. 1, p. 40-46en_HK
dc.identifier.issn0168-3659en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69597-
dc.description.abstractThe commercially available branched polyethyleneimine (PEI) with a molar mass of 25 kD (PEI-25K) is an effective in vitro vector to transfer genes, but its cytotoxicity limits its applications in bio-related research. To solve such an efficiency-versus-cytotoxicity catch-22 problem, the disulfide bond has been previously used to link less toxic short PEI chains (2 kD), but previous literature results are controversial. Recently, we found that it is vitally important to remove both carbon dioxide and water in the linking reaction as well as to control the structure of the resultant chains linked by dithiobis(succinimidyl propionate) (DSP). Under a programmable mixing of PEI and DSP, we can use laser light scattering (LLS) to in-situ monitor the linking reaction kinetics in DMSO in terms of the change of the average molar mass (Mw). Therefore, we were able to withdraw a series of linked PEI chains with different molar masses from one reaction mixture. Two such linked PEI samples (Mw ~ 7 kD, PEI-7K-L and ~ 400 kD, PEI-400K-L) were used to illustrate the effect of the sample preparation and the chain structure on the in vitro gene transfection and cytotoxicity. Our results reveal that PEI-7K-L is less cytotoxic and more effective in the gene transfection than both PEI-25K and Lipofectamine 2000 in the in vitro gene transfection. However, PEI-400K-L has no gene transfection efficiency even though it is non-toxic. © 2009 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrelen_HK
dc.relation.ispartofJournal of Controlled Releaseen_HK
dc.subjectChain structureen_HK
dc.subjectDisulfideen_HK
dc.subjectGene deliveryen_HK
dc.subjectLaser light scatteringen_HK
dc.subjectPolyethyleneimineen_HK
dc.subject.meshCell Survival - drug effects-
dc.subject.meshCross-Linking Reagents - chemistry-
dc.subject.meshDNA - genetics - metabolism-
dc.subject.meshGenetic Vectors-
dc.subject.meshPolyethyleneimine - chemistry - metabolism - toxicity-
dc.titleRevisit the complexation of PEI and DNA - How to make low cytotoxic and highly efficient PEI gene transfection non-viral vectors with a controllable chain length and structure?en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0168-3659&volume=140&issue=1&spage=40&epage=46&date=2009&atitle=Revisit+the+Complexation+of+PEI+And+DNA+-+How+to+Make+Low+Cytotoxic+and+High+Efficient+PEI+Gene+Transfection+Non-viral+Vectors+with+a+Controllable+Chain+Length+and+Structure?en_HK
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jconrel.2009.07.009en_HK
dc.identifier.pmid19625007-
dc.identifier.scopuseid_2-s2.0-70349820287en_HK
dc.identifier.hkuros160394en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70349820287&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume140en_HK
dc.identifier.issue1en_HK
dc.identifier.spage40en_HK
dc.identifier.epage46en_HK
dc.identifier.isiWOS:000271666100007-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridDeng, R=24467381600en_HK
dc.identifier.scopusauthoridYue, Y=35088987100en_HK
dc.identifier.scopusauthoridJin, F=35315971100en_HK
dc.identifier.scopusauthoridChen, Y=24075600300en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK
dc.identifier.scopusauthoridWu, C=7501667902en_HK
dc.identifier.citeulike5243143-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats