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Article: Application of capillary electrophoresis for organic acid analysis in herbal studies

TitleApplication of capillary electrophoresis for organic acid analysis in herbal studies
Authors
KeywordsCapillary electrophoresis
Carboxylic acids
Herbs
Organic anions
Traditional Chinese medicine
Issue Date2001
PublisherWiley - V C H Verlag GmbH & Co KGaA.
Citation
Electrophoresis, 2001, v. 22 n. 11, p. 2242-2250 How to Cite?
AbstractA capillary electrophoresis (CE) procedure has been developed for the separation of 25 inorganic and organic acid anions using a buffer system consisting of 15 mM tris(hydroxymethyl)aminomethane, 3 mM 1,2,4-benzenetricarboxylic acid, 1.5 mM tetraethylenepentaamine (TEPA) and 20% methanol with pH adjusted to 8.4. A good separation of organic acids extracted from a mixture of Chinese traditional medicine (TCM) containing three herbs, Flos chrysthemi, Spica prunellae, and Folium mori was obtained using the procedure developed with satisfactory working range (0.20-77 mg/g), low detection limit (90-190 μg/g), and good repeatability (relative standard deviation 4.47-6.99%, n = 4). A satisfactory extraction of organic acids was achieved within 20 min using 0.1 m NaOH. The addition of TEPA to provide a reduced compounds electroosmotic flow (EOF) environment was shown to remove interfering organic compounds extracted from TCM. The applicability of using organic acids as markers for determining the mixing ratio of constituent herbs for a TCM mixture was investigated using a three-component mixture with a 1:1:1 mixing ratio. A satisfactory mixing ratio of 1.04:1.09:0.98 was obtained using the methodology developed based on organic acids as markers. The application of our method for determining more complicated TCM mixtures has been discussed.
Persistent Identifierhttp://hdl.handle.net/10722/69499
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFung, YSen_HK
dc.contributor.authorTung, HSen_HK
dc.date.accessioned2010-09-06T06:14:13Z-
dc.date.available2010-09-06T06:14:13Z-
dc.date.issued2001en_HK
dc.identifier.citationElectrophoresis, 2001, v. 22 n. 11, p. 2242-2250en_HK
dc.identifier.issn0173-0835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69499-
dc.description.abstractA capillary electrophoresis (CE) procedure has been developed for the separation of 25 inorganic and organic acid anions using a buffer system consisting of 15 mM tris(hydroxymethyl)aminomethane, 3 mM 1,2,4-benzenetricarboxylic acid, 1.5 mM tetraethylenepentaamine (TEPA) and 20% methanol with pH adjusted to 8.4. A good separation of organic acids extracted from a mixture of Chinese traditional medicine (TCM) containing three herbs, Flos chrysthemi, Spica prunellae, and Folium mori was obtained using the procedure developed with satisfactory working range (0.20-77 mg/g), low detection limit (90-190 μg/g), and good repeatability (relative standard deviation 4.47-6.99%, n = 4). A satisfactory extraction of organic acids was achieved within 20 min using 0.1 m NaOH. The addition of TEPA to provide a reduced compounds electroosmotic flow (EOF) environment was shown to remove interfering organic compounds extracted from TCM. The applicability of using organic acids as markers for determining the mixing ratio of constituent herbs for a TCM mixture was investigated using a three-component mixture with a 1:1:1 mixing ratio. A satisfactory mixing ratio of 1.04:1.09:0.98 was obtained using the methodology developed based on organic acids as markers. The application of our method for determining more complicated TCM mixtures has been discussed.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA.en_HK
dc.relation.ispartofElectrophoresisen_HK
dc.subjectCapillary electrophoresisen_HK
dc.subjectCarboxylic acidsen_HK
dc.subjectHerbsen_HK
dc.subjectOrganic anionsen_HK
dc.subjectTraditional Chinese medicineen_HK
dc.titleApplication of capillary electrophoresis for organic acid analysis in herbal studiesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0173-0835&volume=22&spage=2242&epage=2250&date=2001&atitle=Application+of+capillary+electrophoresis+for+organic+acid+analysis+in+herbal+studiesen_HK
dc.identifier.emailFung, YS:ysfung@hku.hken_HK
dc.identifier.authorityFung, YS=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/1522-2683(20017)22:11<2242::AID-ELPS2242>3.0.CO;2-Een_HK
dc.identifier.pmid11504059-
dc.identifier.scopuseid_2-s2.0-0034917063en_HK
dc.identifier.hkuros68481en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034917063&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue11en_HK
dc.identifier.spage2242en_HK
dc.identifier.epage2250en_HK
dc.identifier.isiWOS:000170175800018-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridFung, YS=13309754700en_HK
dc.identifier.scopusauthoridTung, HS=7006351429en_HK

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