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Article: Immunochemical characterization of the functional constituents of Tripterygium wilfordii contributing to its anti-inflammatory property

TitleImmunochemical characterization of the functional constituents of Tripterygium wilfordii contributing to its anti-inflammatory property
Authors
Keywordsβ-hydroxyl
γ-butyrolactone
Structure-activity relationship
Triptolide
Issue Date2008
PublisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEP
Citation
Clinical And Experimental Pharmacology And Physiology, 2008, v. 35 n. 1, p. 55-59 How to Cite?
Abstract
1. Tripterygium wilfordii (TW) contains bioactive compounds that possess immunosuppressive properties. These compounds are considered to be potential drugs in the treatment of acute graft rejections. However, their structure-activity relationships remain unknown. 2. The aim of the present study was to delineate the molecular moieties of triptolide that could account for its ability to inhibit inflammatory responses. In this context, purified TW active compounds (triptolide and triptonide) and synthetic triptolide derivatives were prepared to investigate the structure-activity relationships of triptolide. To this end, rat splenocytes were treated with increasing concentrations of the compounds and then allogenically stimulated using a mixed lymphocyte reaction to determine their antiproliferative activities. From the results, the IC 50 value of each compound was calculated. 3. Modification of the β-hydroxyl group at the C-14 position of the triptolide molecule significantly affected the immunosuppressive activity of T59, as demonstrated by a sevenfold increase of the IC 50. Conversely, reduction of the γ-butyrolactone group in T60 and T61 completely abrogated the antiproliferative effect. Alterations in the C-14 β-hydroxyl and γ-butyrolactone groups also resulted in reduced cytotoxicity. 4. The present findings demonstrate that the C-14 β-hydroxyl and γ-butyrolactone moieties of the triptolide molecule are crucial for its anti-inflammatory properties and cytotoxicity and are responsible for the compound's antiproliferative activity. © 2007 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/69445
ISSN
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, KFen_HK
dc.contributor.authorChan, JKen_HK
dc.contributor.authorChan, KLen_HK
dc.contributor.authorTam, Pen_HK
dc.contributor.authorYang, Den_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorLuk, JMen_HK
dc.date.accessioned2010-09-06T06:13:43Z-
dc.date.available2010-09-06T06:13:43Z-
dc.date.issued2008en_HK
dc.identifier.citationClinical And Experimental Pharmacology And Physiology, 2008, v. 35 n. 1, p. 55-59en_HK
dc.identifier.issn0305-1870en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69445-
dc.description.abstract1. Tripterygium wilfordii (TW) contains bioactive compounds that possess immunosuppressive properties. These compounds are considered to be potential drugs in the treatment of acute graft rejections. However, their structure-activity relationships remain unknown. 2. The aim of the present study was to delineate the molecular moieties of triptolide that could account for its ability to inhibit inflammatory responses. In this context, purified TW active compounds (triptolide and triptonide) and synthetic triptolide derivatives were prepared to investigate the structure-activity relationships of triptolide. To this end, rat splenocytes were treated with increasing concentrations of the compounds and then allogenically stimulated using a mixed lymphocyte reaction to determine their antiproliferative activities. From the results, the IC 50 value of each compound was calculated. 3. Modification of the β-hydroxyl group at the C-14 position of the triptolide molecule significantly affected the immunosuppressive activity of T59, as demonstrated by a sevenfold increase of the IC 50. Conversely, reduction of the γ-butyrolactone group in T60 and T61 completely abrogated the antiproliferative effect. Alterations in the C-14 β-hydroxyl and γ-butyrolactone groups also resulted in reduced cytotoxicity. 4. The present findings demonstrate that the C-14 β-hydroxyl and γ-butyrolactone moieties of the triptolide molecule are crucial for its anti-inflammatory properties and cytotoxicity and are responsible for the compound's antiproliferative activity. © 2007 The Authors.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEPen_HK
dc.relation.ispartofClinical and Experimental Pharmacology and Physiologyen_HK
dc.subjectβ-hydroxylen_HK
dc.subjectγ-butyrolactoneen_HK
dc.subjectStructure-activity relationshipen_HK
dc.subjectTriptolideen_HK
dc.titleImmunochemical characterization of the functional constituents of Tripterygium wilfordii contributing to its anti-inflammatory propertyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0305-1870&volume=35&issue=1&spage=55&epage=59&date=2008&atitle=Immunochemical+characterization+of+the+functional+constituents+of+tripterygium+wilfordii+contributing+to+its+anti-inflammatory+propertyen_HK
dc.identifier.emailTam, P: paultam@hkucc.hku.hken_HK
dc.identifier.emailYang, D: yangdan@hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.authorityChan, KL=rp00572en_HK
dc.identifier.authorityTam, P=rp00060en_HK
dc.identifier.authorityYang, D=rp00825en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1440-1681.2007.04740.xen_HK
dc.identifier.pmid18047628en_HK
dc.identifier.scopuseid_2-s2.0-36649012569en_HK
dc.identifier.hkuros138857en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36649012569&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume35en_HK
dc.identifier.issue1en_HK
dc.identifier.spage55en_HK
dc.identifier.epage59en_HK
dc.identifier.isiWOS:000251249600011-
dc.publisher.placeAustraliaen_HK
dc.identifier.scopusauthoridWong, KF=35081410800en_HK
dc.identifier.scopusauthoridChan, JK=15730226000en_HK
dc.identifier.scopusauthoridChan, KL=36649012569en_HK
dc.identifier.scopusauthoridTam, P=7202539421en_HK
dc.identifier.scopusauthoridYang, D=7404800756en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.citeulike2042897-

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