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Article: Inhibition of Akt/PKB by a COX-2 inhibitor induces apoptosis in gastric cancer cells

TitleInhibition of Akt/PKB by a COX-2 inhibitor induces apoptosis in gastric cancer cells
Authors
KeywordsAkt kinase/protein kinase B
Apoptosis
Caspase
Cyclooxygenase-2 inhibitor SC236
Cytochrome c
SC236 cyclooxygenase-2 inhibitor
Issue Date2006
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/DIG
Citation
Digestion, 2006, v. 73 n. 2-3, p. 75-83 How to Cite?
AbstractBackground/Aim: Inhibition of cyclooxygenase-2 has been proposed to be a potential mechanism for the chemoprevention of gastrointestinal tumors by nonsteroidal anti-inflammatory drugs. This study investigates the mechanisms by which the cyclooxygenase-2 inhibitor SC236 induces apoptosis of gastric cancer cell lines and its downstream signaling pathway. Methods: Two gastric cancer cell lines, AGS and MKN28, were treated with SC236 and assessed for cell growth and apoptosis. The involvement of mitogen-activated protein kinase and Akt kinase/protein kinase B (Akt/PKB) pathways and their downstream signalings were studied in the AGS cell line. Results: SC236 treatment induced apoptosis in gastric cancer cells and caused activation of p38 and stress-activated protein kinase/jun kinase, but down-regulated Akt/PKB. The specific p38 inhibitor SB203580 and the dominant-negative stress-activated protein kinase/jun kinase both failed, while the constitutively active form of Akt/PKB was able to block SC236-induced apoptosis. SC236-induced apoptosis was coupled with release of cytochrome c and activation of caspases. Conclusion: One of the pathways involved in SC-236-induced apoptosis in gastric cancer cells is through down regulation of Akt and then release of cytochrome c. Copyright © 2006 S. Karger AG.
Persistent Identifierhttp://hdl.handle.net/10722/69424
ISSN
2015 Impact Factor: 1.884
2015 SCImago Journal Rankings: 0.953
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFan, XMen_HK
dc.contributor.authorJiang, XHen_HK
dc.contributor.authorGu, Qen_HK
dc.contributor.authorChing, YPen_HK
dc.contributor.authorHe, Hen_HK
dc.contributor.authorXia, HHXen_HK
dc.contributor.authorLin, MCMen_HK
dc.contributor.authorChan, AOOen_HK
dc.contributor.authorYuen, MFen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2010-09-06T06:13:32Z-
dc.date.available2010-09-06T06:13:32Z-
dc.date.issued2006en_HK
dc.identifier.citationDigestion, 2006, v. 73 n. 2-3, p. 75-83en_HK
dc.identifier.issn0012-2823en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69424-
dc.description.abstractBackground/Aim: Inhibition of cyclooxygenase-2 has been proposed to be a potential mechanism for the chemoprevention of gastrointestinal tumors by nonsteroidal anti-inflammatory drugs. This study investigates the mechanisms by which the cyclooxygenase-2 inhibitor SC236 induces apoptosis of gastric cancer cell lines and its downstream signaling pathway. Methods: Two gastric cancer cell lines, AGS and MKN28, were treated with SC236 and assessed for cell growth and apoptosis. The involvement of mitogen-activated protein kinase and Akt kinase/protein kinase B (Akt/PKB) pathways and their downstream signalings were studied in the AGS cell line. Results: SC236 treatment induced apoptosis in gastric cancer cells and caused activation of p38 and stress-activated protein kinase/jun kinase, but down-regulated Akt/PKB. The specific p38 inhibitor SB203580 and the dominant-negative stress-activated protein kinase/jun kinase both failed, while the constitutively active form of Akt/PKB was able to block SC236-induced apoptosis. SC236-induced apoptosis was coupled with release of cytochrome c and activation of caspases. Conclusion: One of the pathways involved in SC-236-induced apoptosis in gastric cancer cells is through down regulation of Akt and then release of cytochrome c. Copyright © 2006 S. Karger AG.en_HK
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/DIGen_HK
dc.relation.ispartofDigestionen_HK
dc.rightsDigestion. Copyright © S Karger AG.en_HK
dc.subjectAkt kinase/protein kinase Ben_HK
dc.subjectApoptosisen_HK
dc.subjectCaspaseen_HK
dc.subjectCyclooxygenase-2 inhibitor SC236en_HK
dc.subjectCytochrome cen_HK
dc.subjectSC236 cyclooxygenase-2 inhibitoren_HK
dc.subject.meshAcridine Orangeen_HK
dc.subject.meshAdenocarcinoma - pathologyen_HK
dc.subject.meshApoptosis - drug effectsen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCaspases - metabolismen_HK
dc.subject.meshCyclooxygenase 2 Inhibitors - pharmacologyen_HK
dc.subject.meshCytochromes c - metabolismen_HK
dc.subject.meshDown-Regulationen_HK
dc.subject.meshEnzyme Activationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMitogen-Activated Protein Kinases - metabolismen_HK
dc.subject.meshProto-Oncogene Proteins c-akt - antagonists & inhibitorsen_HK
dc.subject.meshPyrazoles - pharmacologyen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSignal Transductionen_HK
dc.subject.meshStomach Neoplasms - pathologyen_HK
dc.subject.meshSulfonamides - pharmacologyen_HK
dc.subject.meshTransfectionen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleInhibition of Akt/PKB by a COX-2 inhibitor induces apoptosis in gastric cancer cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0012-2823&volume=73&issue=2-3&spage=75&epage=83&date=2006&atitle=Inhibition+of+Akt/PKB+by+a+COX-2+Inhibitor+Induces+Apoptosis+in+Gastric+Cancer+Cellsen_HK
dc.identifier.emailChing, YP:ypching@hku.hken_HK
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_HK
dc.identifier.emailYuen, MF:mfyuen@hkucc.hku.hken_HK
dc.identifier.emailWong, BCY:bcywong@hku.hken_HK
dc.identifier.authorityChing, YP=rp00469en_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.identifier.authorityYuen, MF=rp00479en_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000092747en_HK
dc.identifier.pmid16641552-
dc.identifier.scopuseid_2-s2.0-33746646010en_HK
dc.identifier.hkuros115688en_HK
dc.identifier.hkuros143403-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33746646010&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume73en_HK
dc.identifier.issue2-3en_HK
dc.identifier.spage75en_HK
dc.identifier.epage83en_HK
dc.identifier.isiWOS:000239427800002-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridFan, XM=35187111100en_HK
dc.identifier.scopusauthoridJiang, XH=36089034900en_HK
dc.identifier.scopusauthoridGu, Q=24469982400en_HK
dc.identifier.scopusauthoridChing, YP=7005431277en_HK
dc.identifier.scopusauthoridHe, H=36185495900en_HK
dc.identifier.scopusauthoridXia, HHX=8757161400en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK
dc.identifier.scopusauthoridChan, AOO=7403167965en_HK
dc.identifier.scopusauthoridYuen, MF=7102031955en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK

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