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Article: In vivo analysis and spatial profiling of phytochemicals in herbal tissue by matrix-assisted laser desorption/ionization mass spectrometry

TitleIn vivo analysis and spatial profiling of phytochemicals in herbal tissue by matrix-assisted laser desorption/ionization mass spectrometry
Authors
Issue Date2007
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ac
Citation
Analytical Chemistry, 2007, v. 79 n. 7, p. 2745-2755 How to Cite?
AbstractMatrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was developed for spatial profiling of phytochemicals and secondary metabolites in integrated herbal tissue without solvent extraction. Abundant alkaloid ions, including (+)-menisperine (m/z 356), magnoflorine (m/z 342), stepharanine (m/z 324), protonated sinomenine (m/z 330), protonated sinomendine (m/z 338), and a metabolite at m/z 314, could be directly desorbed from α-cyano-4- hydroxycinnamic acid- (CHCA-) coated stem tissue of Sinomenium acutum upon N2 laser (337 nm) ablation, while the ion signals desorbed from sinapinic acid- (SA-) coated and 2,5-dihydroxybenzoic acid- (DHB-) coated stem tissue were at least 10 times weaker. Solvent composition in the matrix solution could have significant effects on the ion intensity of the metabolites. Under optimized conditions that maximize the ion intensity and form homogeneous matrix crystals on the tissue surface, spatial distributions of the metabolites localized in different tissue regions, including cortex, phloem, xylem, rim, and pith, and their relative abundances could be semiquantitatively determined. The three metabolites detected at m/z 356, 342, and 314 showed specific distributions in the herbal samples collected from different growing areas, while others were not. By applying principal component analysis (PCA), the characteristic metabolites in specific tissue regions could be easily determined, allowing unambiguous differentiation of the herbal samples from different geographic locations. © 2007 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/69350
ISSN
2014 Impact Factor: 5.636
2014 SCImago Journal Rankings: 2.046
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, KMen_HK
dc.contributor.authorLiang, Zen_HK
dc.contributor.authorLu, Wen_HK
dc.contributor.authorTang, HWen_HK
dc.contributor.authorZhao, Zen_HK
dc.contributor.authorChe, CMen_HK
dc.contributor.authorCheng, YCen_HK
dc.date.accessioned2010-09-06T06:12:51Z-
dc.date.available2010-09-06T06:12:51Z-
dc.date.issued2007en_HK
dc.identifier.citationAnalytical Chemistry, 2007, v. 79 n. 7, p. 2745-2755en_HK
dc.identifier.issn0003-2700en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69350-
dc.description.abstractMatrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was developed for spatial profiling of phytochemicals and secondary metabolites in integrated herbal tissue without solvent extraction. Abundant alkaloid ions, including (+)-menisperine (m/z 356), magnoflorine (m/z 342), stepharanine (m/z 324), protonated sinomenine (m/z 330), protonated sinomendine (m/z 338), and a metabolite at m/z 314, could be directly desorbed from α-cyano-4- hydroxycinnamic acid- (CHCA-) coated stem tissue of Sinomenium acutum upon N2 laser (337 nm) ablation, while the ion signals desorbed from sinapinic acid- (SA-) coated and 2,5-dihydroxybenzoic acid- (DHB-) coated stem tissue were at least 10 times weaker. Solvent composition in the matrix solution could have significant effects on the ion intensity of the metabolites. Under optimized conditions that maximize the ion intensity and form homogeneous matrix crystals on the tissue surface, spatial distributions of the metabolites localized in different tissue regions, including cortex, phloem, xylem, rim, and pith, and their relative abundances could be semiquantitatively determined. The three metabolites detected at m/z 356, 342, and 314 showed specific distributions in the herbal samples collected from different growing areas, while others were not. By applying principal component analysis (PCA), the characteristic metabolites in specific tissue regions could be easily determined, allowing unambiguous differentiation of the herbal samples from different geographic locations. © 2007 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/acen_HK
dc.relation.ispartofAnalytical Chemistryen_HK
dc.titleIn vivo analysis and spatial profiling of phytochemicals in herbal tissue by matrix-assisted laser desorption/ionization mass spectrometryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-2700&volume=79&spage=2745 &epage= 2755&date=2007&atitle=In+Vivo+Analysis+And+Spatial+Profiling+Of+Phytochemicals+In+Herbal+Tissue+By+Matrix-assisted+Laser+Desorption/ionization+Mass+Spectrometryen_HK
dc.identifier.emailNg, KM:kwanmng@hku.hken_HK
dc.identifier.emailLu, W:luwei@hku.hken_HK
dc.identifier.emailChe, CM:cmche@hku.hken_HK
dc.identifier.authorityNg, KM=rp00766en_HK
dc.identifier.authorityLu, W=rp00754en_HK
dc.identifier.authorityChe, CM=rp00670en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/ac062129ien_HK
dc.identifier.pmid17313187-
dc.identifier.scopuseid_2-s2.0-34247092007en_HK
dc.identifier.hkuros126122en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34247092007&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume79en_HK
dc.identifier.issue7en_HK
dc.identifier.spage2745en_HK
dc.identifier.epage2755en_HK
dc.identifier.isiWOS:000245304300015-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNg, KM=26026091100en_HK
dc.identifier.scopusauthoridLiang, Z=8593743800en_HK
dc.identifier.scopusauthoridLu, W=27868087600en_HK
dc.identifier.scopusauthoridTang, HW=16231745000en_HK
dc.identifier.scopusauthoridZhao, Z=9740885000en_HK
dc.identifier.scopusauthoridChe, CM=7102442791en_HK
dc.identifier.scopusauthoridCheng, YC=36041844200en_HK

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