File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Determination of trace metals by capillary electrophoresis

TitleDetermination of trace metals by capillary electrophoresis
Authors
Keywords1,10-Phenanthroline
Capillary electrophoresis
Direct UV detection
Trace metals
Issue Date2001
PublisherWiley - V C H Verlag GmbH & Co KGaA.
Citation
Electrophoresis, 2001, v. 22 n. 11, p. 2192-2200 How to Cite?
AbstractA new analytical procedure is developed using a strong complexing agent, 1,10-phenanthroline (Phen), for direct UV detection of Zn, Mn, Cu, Co, Cd, and Fe at μg/L concentrations in environmental water samples. The metal chelates formed showed different electrophoretic mobilities and solved the comigration problem for capillary electrophoresis (CE) separation of free metal ions. To obtain stable metal-Phen chelates during the capillary zone electrophoresis (CZE) run, both pre-column and on-column complexation are required and threefold excess of Phen over metal ions should be added to the sample. The optimized background electrolyte (BGE) consists of 30 mM hydroxylamine hydrochloride and 0.1% methanol at pH 3.6. Under hydrodynamic sampling, CE run at + 20 kV in 65 cm × 0.05 mm ID fused-silica column with detection at 265 nm, baseline separation, satisfactory working ranges (10 μg/L to 5.5 mg/L), sensitive detection limits (1-3 μg/L), good repeatability for migration times (relative standard deviation, RSD 0.36-0.81%, n = 5), peak area (RSD 3.2-4.2%, n = 5) and peak height (RSD 3.2-4.5%, n = 5) were obtained for the metal cations investigated. The reliability of the method was established by parallel determination using the inductively coupled plasma-atomic emission spectrometry (ICP-AES) method giving results within statistical variation. The procedure developed is shown to provide a quick, sensitive, precise, and economic method for simultaneous determination of metal cations that can form stable chelates with Phen.
Persistent Identifierhttp://hdl.handle.net/10722/69349
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFung, YFen_HK
dc.contributor.authorLau, KMen_HK
dc.date.accessioned2010-09-06T06:12:51Z-
dc.date.available2010-09-06T06:12:51Z-
dc.date.issued2001en_HK
dc.identifier.citationElectrophoresis, 2001, v. 22 n. 11, p. 2192-2200en_HK
dc.identifier.issn0173-0835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69349-
dc.description.abstractA new analytical procedure is developed using a strong complexing agent, 1,10-phenanthroline (Phen), for direct UV detection of Zn, Mn, Cu, Co, Cd, and Fe at μg/L concentrations in environmental water samples. The metal chelates formed showed different electrophoretic mobilities and solved the comigration problem for capillary electrophoresis (CE) separation of free metal ions. To obtain stable metal-Phen chelates during the capillary zone electrophoresis (CZE) run, both pre-column and on-column complexation are required and threefold excess of Phen over metal ions should be added to the sample. The optimized background electrolyte (BGE) consists of 30 mM hydroxylamine hydrochloride and 0.1% methanol at pH 3.6. Under hydrodynamic sampling, CE run at + 20 kV in 65 cm × 0.05 mm ID fused-silica column with detection at 265 nm, baseline separation, satisfactory working ranges (10 μg/L to 5.5 mg/L), sensitive detection limits (1-3 μg/L), good repeatability for migration times (relative standard deviation, RSD 0.36-0.81%, n = 5), peak area (RSD 3.2-4.2%, n = 5) and peak height (RSD 3.2-4.5%, n = 5) were obtained for the metal cations investigated. The reliability of the method was established by parallel determination using the inductively coupled plasma-atomic emission spectrometry (ICP-AES) method giving results within statistical variation. The procedure developed is shown to provide a quick, sensitive, precise, and economic method for simultaneous determination of metal cations that can form stable chelates with Phen.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA.en_HK
dc.relation.ispartofElectrophoresisen_HK
dc.subject1,10-Phenanthrolineen_HK
dc.subjectCapillary electrophoresisen_HK
dc.subjectDirect UV detectionen_HK
dc.subjectTrace metalsen_HK
dc.titleDetermination of trace metals by capillary electrophoresisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0173-0835&volume=22&spage=2192&epage=2200&date=2001&atitle=Determination+of+trace+metals+by+capillary+electrophoresisen_HK
dc.identifier.emailFung, YF:ysfung@hku.hken_HK
dc.identifier.authorityFung, YF=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/1522-2683(20017)22:11<2192::AID-ELPS2192>3.0.CO;2-Aen_HK
dc.identifier.pmid11504052en_HK
dc.identifier.scopuseid_2-s2.0-0034928585en_HK
dc.identifier.hkuros68489en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034928585&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue11en_HK
dc.identifier.spage2192en_HK
dc.identifier.epage2200en_HK
dc.identifier.isiWOS:000170175800011-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridFung, YF=13309754700en_HK
dc.identifier.scopusauthoridLau, KM=7401559826en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats