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Article: Effect of WeiJia on carbon tetrachloride induced chronic liver injury

TitleEffect of WeiJia on carbon tetrachloride induced chronic liver injury
Authors
KeywordsCarbon tetrachloride
Liver fibrosis
WeiJia
Issue Date2006
PublisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htm
Citation
World Journal Of Gastroenterology, 2006, v. 12 n. 12, p. 1912-1917 How to Cite?
AbstractAim: To study the effect of WeiJia on chronic liver injury using carbon tetrachloride (CCl 4) induced liver injury animal model. Methods: Wista r rats weighing 180-220g were randomly divided into three groups: normal control group (Group A), CCl 4 induced liver injury control group (Group B) and CCl 4 induction with WeiJia treatment group (Group C). Each group consisted of 14 rats. Liver damage and fibrosis was induced by subcutaneous injection with 40% CCl 4 in olive oil at 3 mL/kg body weight twice a week for eight weeks for Groups B and C rats whereas olive oil was used for Group A rats. Starting from the third week, Group C rats also received daily intraperitoneal injection of WeiJia at a dose of 1.25 μg/kg body weight. Animals were sacrificed at the fifth week (4 male, 3 female), and eighth week (4 male, 3 female) respectively. Degree of fibrosis were measured and serological markers for liver fibrosis and function including hyaluronic acid (HA), type IV collagen (CIV), γ-glutamyl transferase (γ-GT), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. Alpha smooth muscle actin (α-SMA) and proliferating cell nuclear antigen (PCNA) immunohistochemistry were also performed. Results: CCl 4 induction led to the damage of liver and development of fibrosis in Group B and Group C rats when compared to Group A rats. The treatment of WeiJia in Group C rats could reduce the fibrosis condition significantly compared to Group B rats. The effect could be observed after three weeks of treatment and was more obvious after eight weeks of treatment. Serum HA, CIV, ALT, AST and γ-GT levels after eight weeks of treatment for Group C rats were 58±22 μg/L (P<0.01), 57±21 μg/L (P<0.01), 47±10 U/L (P<0.01), 139±13 U/L (P<0.05) and 52±21 U/L (P> 0.05) respectively, similar to normal control group (Group A), but significantly different from CCl 4 induced liver injury control group (Group B). An increase in PCNA and decrease in α-SMA expression level was also observed. Conclusion: WeiJia could improve liver function and reduce liver fibrosis which might be through the inhibition of stellate cell activity. © 2006 The WJG Press. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/69299
ISSN
2015 Impact Factor: 2.787
2015 SCImago Journal Rankings: 1.076
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, PYen_HK
dc.contributor.authorZhang, Qen_HK
dc.contributor.authorZhang, YOen_HK
dc.contributor.authorBai, GRen_HK
dc.contributor.authorLin, MCMen_HK
dc.contributor.authorChan, Ben_HK
dc.contributor.authorFong, CCen_HK
dc.contributor.authorShi, Len_HK
dc.contributor.authorShi, YFen_HK
dc.contributor.authorChun, Jen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorYang, MSen_HK
dc.date.accessioned2010-09-06T06:12:23Z-
dc.date.available2010-09-06T06:12:23Z-
dc.date.issued2006en_HK
dc.identifier.citationWorld Journal Of Gastroenterology, 2006, v. 12 n. 12, p. 1912-1917en_HK
dc.identifier.issn1007-9327en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69299-
dc.description.abstractAim: To study the effect of WeiJia on chronic liver injury using carbon tetrachloride (CCl 4) induced liver injury animal model. Methods: Wista r rats weighing 180-220g were randomly divided into three groups: normal control group (Group A), CCl 4 induced liver injury control group (Group B) and CCl 4 induction with WeiJia treatment group (Group C). Each group consisted of 14 rats. Liver damage and fibrosis was induced by subcutaneous injection with 40% CCl 4 in olive oil at 3 mL/kg body weight twice a week for eight weeks for Groups B and C rats whereas olive oil was used for Group A rats. Starting from the third week, Group C rats also received daily intraperitoneal injection of WeiJia at a dose of 1.25 μg/kg body weight. Animals were sacrificed at the fifth week (4 male, 3 female), and eighth week (4 male, 3 female) respectively. Degree of fibrosis were measured and serological markers for liver fibrosis and function including hyaluronic acid (HA), type IV collagen (CIV), γ-glutamyl transferase (γ-GT), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. Alpha smooth muscle actin (α-SMA) and proliferating cell nuclear antigen (PCNA) immunohistochemistry were also performed. Results: CCl 4 induction led to the damage of liver and development of fibrosis in Group B and Group C rats when compared to Group A rats. The treatment of WeiJia in Group C rats could reduce the fibrosis condition significantly compared to Group B rats. The effect could be observed after three weeks of treatment and was more obvious after eight weeks of treatment. Serum HA, CIV, ALT, AST and γ-GT levels after eight weeks of treatment for Group C rats were 58±22 μg/L (P<0.01), 57±21 μg/L (P<0.01), 47±10 U/L (P<0.01), 139±13 U/L (P<0.05) and 52±21 U/L (P> 0.05) respectively, similar to normal control group (Group A), but significantly different from CCl 4 induced liver injury control group (Group B). An increase in PCNA and decrease in α-SMA expression level was also observed. Conclusion: WeiJia could improve liver function and reduce liver fibrosis which might be through the inhibition of stellate cell activity. © 2006 The WJG Press. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htmen_HK
dc.relation.ispartofWorld Journal of Gastroenterologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCarbon tetrachlorideen_HK
dc.subjectLiver fibrosisen_HK
dc.subjectWeiJiaen_HK
dc.titleEffect of WeiJia on carbon tetrachloride induced chronic liver injuryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1007-9327&volume=12&issue=12&spage=1912&epage=1917&date=2006&atitle=Effect+of+WeiJia+on+carbon+tetrachloride+induced+chronic+liver+injuryen_HK
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3748/wjg.v12.i12.1912-
dc.identifier.pmid16609998-
dc.identifier.pmcidPMC4087517-
dc.identifier.scopuseid_2-s2.0-33645963113en_HK
dc.identifier.hkuros115278en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33645963113&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue12en_HK
dc.identifier.spage1912en_HK
dc.identifier.epage1917en_HK
dc.identifier.isiWOS:000239996000012-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCheung, PY=7202595426en_HK
dc.identifier.scopusauthoridZhang, Q=35515964300en_HK
dc.identifier.scopusauthoridZhang, YO=16176894000en_HK
dc.identifier.scopusauthoridBai, GR=7103085757en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK
dc.identifier.scopusauthoridChan, B=13005652300en_HK
dc.identifier.scopusauthoridFong, CC=11739503800en_HK
dc.identifier.scopusauthoridShi, L=55254757000en_HK
dc.identifier.scopusauthoridShi, YF=7404963405en_HK
dc.identifier.scopusauthoridChun, J=13005621200en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridYang, MS=35204210300en_HK

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