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Article: Specific postcolumn detection method for HPLC assay of homocysteine based on aggregation of fluorosurfactant-capped gold nanoparticles

TitleSpecific postcolumn detection method for HPLC assay of homocysteine based on aggregation of fluorosurfactant-capped gold nanoparticles
Authors
Issue Date2007
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ac
Citation
Analytical Chemistry, 2007, v. 79 n. 2, p. 666-672 How to Cite?
AbstractGold nanoparticles (GNPs) capped with nonionic fluorosurfactant molecules (Zonyl FSN) were synthesized, and with the colloidal solution as a probe reagent, a new postcolumn colorimetric detection method for HPLC assay of homocysteine (Hcy) has been developed. The FSN-capped GNPs exhibited excellent stability in aqueous solutions, even in the presence of high salt. The aggregation of the GNPs could be induced by either Hey or cysteine, resulting in an absorption decrease of the colloidal solution at 525 nm and an absorption increase at longer wavelengths (600-700 nm); however, the GNPs did not respond to other amino acids and biomolecules such as glutathione, cysteinylglycine, and glucose. Under optimal conditions (i.e., high salt, neutral pH, and ∼70 °C), the color change of the GNP solution could almost complete (∼90%) within ∼30 s upon the addition of Hcy. The high selectivity and very fast kinetics of the reaction make it a promising system for HPLC postcolumn detection. The new technique has been employed to determine total Hey levels in human urine and plasma samples, and the results are satisfactory. © 2007 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/69241
ISSN
2015 Impact Factor: 5.886
2015 SCImago Journal Rankings: 2.369
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Cen_HK
dc.contributor.authorZu, Yen_HK
dc.contributor.authorYam, VWWen_HK
dc.date.accessioned2010-09-06T06:11:52Z-
dc.date.available2010-09-06T06:11:52Z-
dc.date.issued2007en_HK
dc.identifier.citationAnalytical Chemistry, 2007, v. 79 n. 2, p. 666-672en_HK
dc.identifier.issn0003-2700en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69241-
dc.description.abstractGold nanoparticles (GNPs) capped with nonionic fluorosurfactant molecules (Zonyl FSN) were synthesized, and with the colloidal solution as a probe reagent, a new postcolumn colorimetric detection method for HPLC assay of homocysteine (Hcy) has been developed. The FSN-capped GNPs exhibited excellent stability in aqueous solutions, even in the presence of high salt. The aggregation of the GNPs could be induced by either Hey or cysteine, resulting in an absorption decrease of the colloidal solution at 525 nm and an absorption increase at longer wavelengths (600-700 nm); however, the GNPs did not respond to other amino acids and biomolecules such as glutathione, cysteinylglycine, and glucose. Under optimal conditions (i.e., high salt, neutral pH, and ∼70 °C), the color change of the GNP solution could almost complete (∼90%) within ∼30 s upon the addition of Hcy. The high selectivity and very fast kinetics of the reaction make it a promising system for HPLC postcolumn detection. The new technique has been employed to determine total Hey levels in human urine and plasma samples, and the results are satisfactory. © 2007 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/acen_HK
dc.relation.ispartofAnalytical Chemistryen_HK
dc.titleSpecific postcolumn detection method for HPLC assay of homocysteine based on aggregation of fluorosurfactant-capped gold nanoparticlesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-2700&volume=79&spage=666&epage=672&date=2007&atitle=Specific+Postcolumn+Detection+Method+for+HPLC+Assay+of+Homocysteine+Based+on+Aggregation+of+Fluorosurfactant-Capped+Gold+Nanoparticlesen_HK
dc.identifier.emailYam, VWW:wwyam@hku.hken_HK
dc.identifier.authorityYam, VWW=rp00822en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/ac061513cen_HK
dc.identifier.pmid17222035-
dc.identifier.scopuseid_2-s2.0-33846187228en_HK
dc.identifier.hkuros130579en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846187228&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume79en_HK
dc.identifier.issue2en_HK
dc.identifier.spage666en_HK
dc.identifier.epage672en_HK
dc.identifier.isiWOS:000243415800035-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLu, C=36013065000en_HK
dc.identifier.scopusauthoridZu, Y=7006348826en_HK
dc.identifier.scopusauthoridYam, VWW=18539304700en_HK

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