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Article: Accumulation of isoflavone genistin in transgenic tomato plants overexpressing a soybean isoflavone synthase gene

TitleAccumulation of isoflavone genistin in transgenic tomato plants overexpressing a soybean isoflavone synthase gene
Authors
KeywordsGenistin
Isoflavone synthase
Metabolic engineering
Transgenic tomato
Issue Date2008
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
Citation
Journal Of Agricultural And Food Chemistry, 2008, v. 56 n. 14, p. 5655-5661 How to Cite?
AbstractIsoflavones are legume-specific flavonoids best known for their potential cancer preventive and phytoestrogenic properties. In this study, we attempted to engineer the isoflavone pathway in the popular fruit crop tomato (Solanum lycopersicum L). Tomato plants were transformed with a soybean (Glycine max L) isoflavone synthase (GmIFS2) cDNA under the control of the cauliflower mosaic virus 35S promoter. LC-MS/MS analysis demonstrated the presence of genistin (genistein 7-O-glucoside) as the major isoflavone metabolite in the transgenic plants. Substantial amounts of genistin (up to 90 nmol/g FW) were found in leaves, while the levels were marginally detectable (less than 0.5 nmol/g FW) in fruit peels. In either case, no drastic variations in endogenous phenolic contents were observed. Fruit peels were found to accumulate high levels of naringenin chalcone, implicating the limitation of naringenin substrates for isoflavone synthesis. Our results suggested that tomato plants could be engineered to produce isoflavones without comprising the levels of endogenous flavonols, which are also health-beneficial, but it may be necessary to enhance the expression levels of chalcone isomerase simultaneously to achieve significant yields in edible tissues such as fruit peels. © 2008 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/69185
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.246
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShih, CHen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorChu, IKen_HK
dc.contributor.authorLo, Cen_HK
dc.date.accessioned2010-09-06T06:11:21Z-
dc.date.available2010-09-06T06:11:21Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 2008, v. 56 n. 14, p. 5655-5661en_HK
dc.identifier.issn0021-8561en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69185-
dc.description.abstractIsoflavones are legume-specific flavonoids best known for their potential cancer preventive and phytoestrogenic properties. In this study, we attempted to engineer the isoflavone pathway in the popular fruit crop tomato (Solanum lycopersicum L). Tomato plants were transformed with a soybean (Glycine max L) isoflavone synthase (GmIFS2) cDNA under the control of the cauliflower mosaic virus 35S promoter. LC-MS/MS analysis demonstrated the presence of genistin (genistein 7-O-glucoside) as the major isoflavone metabolite in the transgenic plants. Substantial amounts of genistin (up to 90 nmol/g FW) were found in leaves, while the levels were marginally detectable (less than 0.5 nmol/g FW) in fruit peels. In either case, no drastic variations in endogenous phenolic contents were observed. Fruit peels were found to accumulate high levels of naringenin chalcone, implicating the limitation of naringenin substrates for isoflavone synthesis. Our results suggested that tomato plants could be engineered to produce isoflavones without comprising the levels of endogenous flavonols, which are also health-beneficial, but it may be necessary to enhance the expression levels of chalcone isomerase simultaneously to achieve significant yields in edible tissues such as fruit peels. © 2008 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcauen_HK
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_HK
dc.subjectGenistinen_HK
dc.subjectIsoflavone synthaseen_HK
dc.subjectMetabolic engineeringen_HK
dc.subjectTransgenic tomatoen_HK
dc.titleAccumulation of isoflavone genistin in transgenic tomato plants overexpressing a soybean isoflavone synthase geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-8561&volume=56&spage=5655&epage=5661&date=2008&atitle=Accumulation+of+isoflavone+genistin+in+transgenic+tomato+plants+overexpressing+a+soybean+isoflavone+synthase+geneen_HK
dc.identifier.emailWang, M: mfwang@hku.hken_HK
dc.identifier.emailChu, IK: ivankchu@hku.hken_HK
dc.identifier.emailLo, C: clivelo@hkucc.hku.hken_HK
dc.identifier.authorityWang, M=rp00800en_HK
dc.identifier.authorityChu, IK=rp00683en_HK
dc.identifier.authorityLo, C=rp00751en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/jf800423uen_HK
dc.identifier.pmid18540614-
dc.identifier.scopuseid_2-s2.0-49049088706en_HK
dc.identifier.hkuros142052en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-49049088706&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume56en_HK
dc.identifier.issue14en_HK
dc.identifier.spage5655en_HK
dc.identifier.epage5661en_HK
dc.identifier.isiWOS:000257721400031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShih, CH=55233136000en_HK
dc.identifier.scopusauthoridChen, Y=37068537700en_HK
dc.identifier.scopusauthoridWang, M=7406691844en_HK
dc.identifier.scopusauthoridChu, IK=7103327484en_HK
dc.identifier.scopusauthoridLo, C=15737175700en_HK

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