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Article: Nonionic surfactant-capped gold nanoparticles as postcolumn reagents for high-performance liquid chromatography assay of low-molecular-mass biothiols

TitleNonionic surfactant-capped gold nanoparticles as postcolumn reagents for high-performance liquid chromatography assay of low-molecular-mass biothiols
Authors
KeywordsGold nanoparticles
High-performance liquid chromatography
Low-molecular-mass biothiols
Nonionic surfactant
Postcolumn reagents
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2007, v. 1163 n. 1-2, p. 328-332 How to Cite?
AbstractGold nanoparticles (GNPs) have been stabilized with nonionic surfactant ligands, i.e., Brij @ 35, and their aggregation could be induced rapidly and selectively by biologically active low-molecular-mass thiols including sulphydryl-containing amino acids (cysteine and homocysteine) and small peptides (glutathione, cysteinylglycine, and glutamylcysteine). A new postcolumn detection method has been developed for high-performance liquid chromatography (HPLC) assay of these small biothiols based on the analyte-induced aggregation of the GNPs. Compared with conventional thiol-reactive probes, the GNP colloids are easier to prepare, much more stable in aqueous solution over a wide pH range and at ambient temperature, and exhibit relatively high selectivity toward small biothiols. The analysis of human urine samples demonstrated that the proposed method is promising in HPLC assay of the small thiol molecules in biological fluids. © 2007 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/69128
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Cen_HK
dc.contributor.authorZu, Yen_HK
dc.contributor.authorYam, VWWen_HK
dc.date.accessioned2010-09-06T06:10:49Z-
dc.date.available2010-09-06T06:10:49Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Chromatography A, 2007, v. 1163 n. 1-2, p. 328-332en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/69128-
dc.description.abstractGold nanoparticles (GNPs) have been stabilized with nonionic surfactant ligands, i.e., Brij @ 35, and their aggregation could be induced rapidly and selectively by biologically active low-molecular-mass thiols including sulphydryl-containing amino acids (cysteine and homocysteine) and small peptides (glutathione, cysteinylglycine, and glutamylcysteine). A new postcolumn detection method has been developed for high-performance liquid chromatography (HPLC) assay of these small biothiols based on the analyte-induced aggregation of the GNPs. Compared with conventional thiol-reactive probes, the GNP colloids are easier to prepare, much more stable in aqueous solution over a wide pH range and at ambient temperature, and exhibit relatively high selectivity toward small biothiols. The analysis of human urine samples demonstrated that the proposed method is promising in HPLC assay of the small thiol molecules in biological fluids. © 2007 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectGold nanoparticlesen_HK
dc.subjectHigh-performance liquid chromatographyen_HK
dc.subjectLow-molecular-mass biothiolsen_HK
dc.subjectNonionic surfactanten_HK
dc.subjectPostcolumn reagentsen_HK
dc.titleNonionic surfactant-capped gold nanoparticles as postcolumn reagents for high-performance liquid chromatography assay of low-molecular-mass biothiolsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1163&spage=328&epage=332&date=2007&atitle=Nonionic+Surfactant-capped+Gold+Nanoparticles+as+Postcolumn+Reagents+for+High-performance+Liquid+Chromatography+Assay+of+Low-molecular-mass+Biothiols+en_HK
dc.identifier.emailYam, VWW:wwyam@hku.hken_HK
dc.identifier.authorityYam, VWW=rp00822en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chroma.2007.07.045en_HK
dc.identifier.pmid17689546-
dc.identifier.scopuseid_2-s2.0-34547837525en_HK
dc.identifier.hkuros136472en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34547837525&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1163en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage328en_HK
dc.identifier.epage332en_HK
dc.identifier.isiWOS:000249310800034-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLu, C=36013065000en_HK
dc.identifier.scopusauthoridZu, Y=7006348826en_HK
dc.identifier.scopusauthoridYam, VWW=18539304700en_HK

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