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Article: Rapid detection of Salmonella enteritidis by piezoelectric immunosensor

TitleRapid detection of Salmonella enteritidis by piezoelectric immunosensor
Authors
KeywordsImmunosensor
Piezoelectric crystal
Salmonella enteritidis
Issue Date2001
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/microc
Citation
Microchemical Journal, 2001, v. 68 n. 1, p. 21-27 How to Cite?
AbstractComparative testing of three different coatings for the immobilization of the anti-Salmonella enteritidis antibody onto piezoelectric (PZ) crystals with gold, silver and palladium electrodes was done. In terms of stability, simplicity and magnitude of the frequency change caused by antibody binding to the coating, polyethyleneimine film gave the better results for the immobilization of antibody as compared with the (γ-aminopropyl)trimethoxysilane film or the self-assembled monolayer of 3-mercaptopropionic acid. To reduce the production cost, the small-sized crystal with silver electrodes was used to fabricate the disposable antibody-modified PZ transducer. The rapid detection of Salmonella enteritidis was successfully performed using the antibody-modified PZ immunosensor. A detection limit of 1 × 105 cells/ml and an assay time of 35 min were achieved. © 2001 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/69104
ISSN
2015 Impact Factor: 2.893
2015 SCImago Journal Rankings: 0.930
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSi, SHen_HK
dc.contributor.authorLi, Xen_HK
dc.contributor.authorFung, YSen_HK
dc.contributor.authorZhu, DRen_HK
dc.date.accessioned2010-09-06T06:10:36Z-
dc.date.available2010-09-06T06:10:36Z-
dc.date.issued2001en_HK
dc.identifier.citationMicrochemical Journal, 2001, v. 68 n. 1, p. 21-27en_HK
dc.identifier.issn0026-265Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/69104-
dc.description.abstractComparative testing of three different coatings for the immobilization of the anti-Salmonella enteritidis antibody onto piezoelectric (PZ) crystals with gold, silver and palladium electrodes was done. In terms of stability, simplicity and magnitude of the frequency change caused by antibody binding to the coating, polyethyleneimine film gave the better results for the immobilization of antibody as compared with the (γ-aminopropyl)trimethoxysilane film or the self-assembled monolayer of 3-mercaptopropionic acid. To reduce the production cost, the small-sized crystal with silver electrodes was used to fabricate the disposable antibody-modified PZ transducer. The rapid detection of Salmonella enteritidis was successfully performed using the antibody-modified PZ immunosensor. A detection limit of 1 × 105 cells/ml and an assay time of 35 min were achieved. © 2001 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/microcen_HK
dc.relation.ispartofMicrochemical Journalen_HK
dc.rightsMicrochemical Journal. Copyright © Elsevier Inc.en_HK
dc.subjectImmunosensoren_HK
dc.subjectPiezoelectric crystalen_HK
dc.subjectSalmonella enteritidisen_HK
dc.titleRapid detection of Salmonella enteritidis by piezoelectric immunosensoren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0026-265X&volume=68&spage=21&epage=27&date=2001&atitle=Rapid+detection+of+Salmonella+enteritidis+by+piezoelectric+immunosensoren_HK
dc.identifier.emailFung, YS:ysfung@hku.hken_HK
dc.identifier.authorityFung, YS=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0026-265X(00)00167-3en_HK
dc.identifier.scopuseid_2-s2.0-0035141202en_HK
dc.identifier.hkuros62501en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035141202&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume68en_HK
dc.identifier.issue1en_HK
dc.identifier.spage21en_HK
dc.identifier.epage27en_HK
dc.identifier.isiWOS:000166821300003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSi, SH=7006049232en_HK
dc.identifier.scopusauthoridLi, X=27171835600en_HK
dc.identifier.scopusauthoridFung, YS=13309754700en_HK
dc.identifier.scopusauthoridZhu, DR=7403599128en_HK

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