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Article: Separation of deoxyribonucleic acid in glucose-hydroxy-propylmethyl cellulose solution by capillary electrophoresis
Title | Separation of deoxyribonucleic acid in glucose-hydroxy-propylmethyl cellulose solution by capillary electrophoresis 毛細管電泳中葡萄糖- 羥丙基甲基纖維素体系分離脫氧核糖核酸 |
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Authors | |
Keywords | Capillary electrophoresis (毛细管电泳) Deoxyribonucleic acid fragment (脱氧核糖核酸) Glucose (葡萄糖) |
Issue Date | 2001 |
Publisher | Chinese Academy of Sciences: Dalian Institute of Chemical Physics (中國科學院大連化學物理研究所). |
Citation | Chinese Journal of Analytical Chemistry, 2001, v. 5 n. 29, p. 502-506 How to Cite? 分析化學, 2001, v. 5 n. 29, p. 502-506 How to Cite? |
Abstract | Glycerol, mannitol has been used as additives for the separation of deoxyribonucleic acid (DNA) fragments in entangled solution by capillary electrophoresis. Differing from glycerol and mannitol, the structure of glucose is a ring with 5 hydroxyl groups. This structure helps it easily to form complex with boric acid and hydroxy propyl methylcellulose (HPMC). On the other hand, glucose has large space obstacle compared with glycerol and mannitol. So glucose can markedly improve separation of DNA in HPMC sol... 葡萄糖作为羟丙基甲基纤维素( Hydroxypropylmethyl cellulose, HPMC) 筛分体系的添加剂, 可以改 善该体系在低浓度时分离脱氧核糖核酸( DNA) 的能力。研究了硼酸浓度和pH 值对葡萄糖- 羟丙基甲基纤维素体系分离性能的影响; 并将葡萄糖与其它添加剂如甘油、甘露醇对分离的影响作了比较, 葡萄糖特有的环状结构使得其对羟丙基甲基纤维素体系分离能力的提高更为显著。 |
Persistent Identifier | http://hdl.handle.net/10722/69080 |
ISSN | 2023 Impact Factor: 1.2 2023 SCImago Journal Rankings: 0.355 |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yan, J | en_HK |
dc.contributor.author | Lin, B | en_HK |
dc.contributor.author | Fung, Y | en_HK |
dc.date.accessioned | 2010-09-06T06:10:22Z | - |
dc.date.available | 2010-09-06T06:10:22Z | - |
dc.date.issued | 2001 | en_HK |
dc.identifier.citation | Chinese Journal of Analytical Chemistry, 2001, v. 5 n. 29, p. 502-506 | en_HK |
dc.identifier.citation | 分析化學, 2001, v. 5 n. 29, p. 502-506 | - |
dc.identifier.issn | 0253-3820 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/69080 | - |
dc.description.abstract | Glycerol, mannitol has been used as additives for the separation of deoxyribonucleic acid (DNA) fragments in entangled solution by capillary electrophoresis. Differing from glycerol and mannitol, the structure of glucose is a ring with 5 hydroxyl groups. This structure helps it easily to form complex with boric acid and hydroxy propyl methylcellulose (HPMC). On the other hand, glucose has large space obstacle compared with glycerol and mannitol. So glucose can markedly improve separation of DNA in HPMC sol... | en_HK |
dc.description.abstract | 葡萄糖作为羟丙基甲基纤维素( Hydroxypropylmethyl cellulose, HPMC) 筛分体系的添加剂, 可以改 善该体系在低浓度时分离脱氧核糖核酸( DNA) 的能力。研究了硼酸浓度和pH 值对葡萄糖- 羟丙基甲基纤维素体系分离性能的影响; 并将葡萄糖与其它添加剂如甘油、甘露醇对分离的影响作了比较, 葡萄糖特有的环状结构使得其对羟丙基甲基纤维素体系分离能力的提高更为显著。 | - |
dc.language | eng | en_HK |
dc.publisher | Chinese Academy of Sciences: Dalian Institute of Chemical Physics (中國科學院大連化學物理研究所). | en_HK |
dc.relation.ispartof | Chinese Journal of Analytical Chemistry | en_HK |
dc.relation.ispartof | 分析化學 | - |
dc.subject | Capillary electrophoresis (毛细管电泳) | en_HK |
dc.subject | Deoxyribonucleic acid fragment (脱氧核糖核酸) | en_HK |
dc.subject | Glucose (葡萄糖) | en_HK |
dc.title | Separation of deoxyribonucleic acid in glucose-hydroxy-propylmethyl cellulose solution by capillary electrophoresis | en_HK |
dc.title | 毛細管電泳中葡萄糖- 羥丙基甲基纖維素体系分離脫氧核糖核酸 | - |
dc.type | Article | en_HK |
dc.identifier.email | Fung, Y: ysfung@hku.hk | en_HK |
dc.identifier.authority | Fung, Y=rp00697 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.scopus | eid_2-s2.0-84880574993 | en_HK |
dc.identifier.hkuros | 62544 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-10044291039&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 5 | en_HK |
dc.identifier.issue | 29 | en_HK |
dc.identifier.spage | 502 | en_HK |
dc.identifier.epage | 506 | en_HK |
dc.publisher.place | Dalian (大連) | en_HK |
dc.identifier.scopusauthorid | Fung, Y=13309754700 | en_HK |
dc.identifier.scopusauthorid | Lin, B=7403508047 | en_HK |
dc.identifier.scopusauthorid | Jin, Y=39861604500 | en_HK |
dc.customcontrol.immutable | csl 130723 | - |
dc.identifier.issnl | 0253-3820 | - |