Article: Identification of flavone phytoalexins and a pathogen-inducible flavone synthase II gene (SbFNSII) in sorghum
File Download
Links for fulltext
(May Require Subscription)
(May Require Subscription)
- Publisher Website: 10.1093/jxb/erp364
- Scopus: eid_2-s2.0-77649244513
- PMID: 20007684
- Find via
Supplementary
-
Citations:
-
Appears in Collections:
| Title | Identification of flavone phytoalexins and a pathogen-inducible flavone synthase II gene (SbFNSII) in sorghum | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Du, Y1 Chu, H1 Wang, M1 Chu, IK1 Lo, C1 | ||||||
| Keywords | Colletotrichum sublineolum CYP93G3 Flavone synthase II Flavones Sorghum | ||||||
| Issue Date | 2010 | ||||||
| Publisher | Oxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/ | ||||||
| Citation | Journal Of Experimental Botany, 2010, v. 61 n. 4, p. 983-994 [How to Cite?] DOI: http://dx.doi.org/10.1093/jxb/erp364 | ||||||
| Abstract | Following inoculation with the anthracnose pathogen Colletotrichum sublineolum, seedlings of the sorghum resistant cultivar SC748-5 showed more rapid and elevated accumulation of luteolin than the susceptible cultivar BTx623. On the other hand, apigenin was the major flavone detected in infected BTx623 seedlings. Luteolin was demonstrated to show stronger inhibition of spore germination of C. sublineolum than apigenin. Because of their pathogen-inducible and antifungal nature, both flavone aglycones are considered sorghum phytoalexins. The key enzyme responsible for flavone biosynthesis has not been characterized in monocots. A sorghum pathogen-inducible gene encoding a cytochrome P450 protein (CYP93G3) in the uncharacterized CYP93G subfamily was identified. Transgenic expression of the P450 gene in Arabidopsis demonstrated that the encoded protein is a functional flavone synthase (FNS) II in planta. The sorghum gene was then termed SbFNSII. It is a single-copy gene located on chromosome 2 and the first FNSII gene characterized in a monocot. Metabolite analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in precursor ion scan mode revealed the accumulation of 2-hydroxynaringenin and 2-hydroxyeriodictyol hexosides in the transgenic Arabidopsis plants. Hence, SbFNSII appears to share a similar catalytic mechanism with the licorice and Medicago truncatula FNSIIs (CYP93B subfamily) by converting flavanones to flavone through the formation of 2-hydroxyflavanones. | ||||||
| ISSN | 0022-0957 2011 Impact Factor: 5.364 2011 SCImago Journal Rankings: 0.426 | ||||||
| DOI | http://dx.doi.org/10.1093/jxb/erp364 | ||||||
| ISI Accession Number ID | WOS:000275567300005
Funding Information: This work was supported by the Research Grants Council of the Hong Kong Special Administrative Region, China (grant no. HKU7527/06M) and The University of Hong Kong Seed Funding program (project code 200711159025). | ||||||
| PubMed Central ID | PMC2826645 | ||||||
| References | References in Scopus | ||||||
| Grants | Promoter analysis of a pathogen-inducible dihydroflavonol reductase gene in sorghum |
| dc.contributor.author | Du, Y | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Chu, H | ||||||
| dc.contributor.author | Wang, M | ||||||
| dc.contributor.author | Chu, IK | ||||||
| dc.contributor.author | Lo, C | ||||||
| dc.date.accessioned | 2010-09-06T06:10:15Z | ||||||
| dc.date.available | 2010-09-06T06:10:15Z | ||||||
| dc.date.issued | 2010 | ||||||
| dc.description.abstract | Following inoculation with the anthracnose pathogen Colletotrichum sublineolum, seedlings of the sorghum resistant cultivar SC748-5 showed more rapid and elevated accumulation of luteolin than the susceptible cultivar BTx623. On the other hand, apigenin was the major flavone detected in infected BTx623 seedlings. Luteolin was demonstrated to show stronger inhibition of spore germination of C. sublineolum than apigenin. Because of their pathogen-inducible and antifungal nature, both flavone aglycones are considered sorghum phytoalexins. The key enzyme responsible for flavone biosynthesis has not been characterized in monocots. A sorghum pathogen-inducible gene encoding a cytochrome P450 protein (CYP93G3) in the uncharacterized CYP93G subfamily was identified. Transgenic expression of the P450 gene in Arabidopsis demonstrated that the encoded protein is a functional flavone synthase (FNS) II in planta. The sorghum gene was then termed SbFNSII. It is a single-copy gene located on chromosome 2 and the first FNSII gene characterized in a monocot. Metabolite analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in precursor ion scan mode revealed the accumulation of 2-hydroxynaringenin and 2-hydroxyeriodictyol hexosides in the transgenic Arabidopsis plants. Hence, SbFNSII appears to share a similar catalytic mechanism with the licorice and Medicago truncatula FNSIIs (CYP93B subfamily) by converting flavanones to flavone through the formation of 2-hydroxyflavanones. | ||||||
| dc.description.grant | Promoter analysis of a pathogen-inducible dihydroflavonol reductase gene in sorghum | ||||||
| dc.description.grantcode | 97921 | ||||||
| dc.description.nature | published_or_final_version | ||||||
| dc.identifier.citation | Journal Of Experimental Botany, 2010, v. 61 n. 4, p. 983-994 [How to Cite?] DOI: http://dx.doi.org/10.1093/jxb/erp364 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1093/jxb/erp364 | ||||||
| dc.identifier.epage | 994 | ||||||
| dc.identifier.hkuros | 169748 | ||||||
| dc.identifier.isi | WOS:000275567300005
Funding Information: This work was supported by the Research Grants Council of the Hong Kong Special Administrative Region, China (grant no. HKU7527/06M) and The University of Hong Kong Seed Funding program (project code 200711159025). | ||||||
| dc.identifier.issn | 0022-0957 2011 Impact Factor: 5.364 2011 SCImago Journal Rankings: 0.426 | ||||||
| dc.identifier.issue | 4 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.pmcid | PMC2826645 | ||||||
| dc.identifier.pmid | 20007684 | ||||||
| dc.identifier.scopus | eid_2-s2.0-77649244513 | ||||||
| dc.identifier.spage | 983 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/69068 | ||||||
| dc.identifier.volume | 61 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Oxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/ | ||||||
| dc.publisher.place | United Kingdom | ||||||
| dc.relation.ispartof | Journal of Experimental Botany | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Cytochrome P-450 Enzyme System - chemistry - genetics - metabolism | ||||||
| dc.subject.mesh | Flavones - biosynthesis | ||||||
| dc.subject.mesh | Plant Proteins - chemistry - genetics - metabolism | ||||||
| dc.subject.mesh | Sorghum - enzymology - genetics - metabolism - microbiology | ||||||
| dc.subject.mesh | Terpenes - metabolism | ||||||
| dc.subject | Colletotrichum sublineolum | ||||||
| dc.subject | CYP93G3 | ||||||
| dc.subject | Flavone synthase II | ||||||
| dc.subject | Flavones | ||||||
| dc.subject | Sorghum | ||||||
| dc.title | Identification of flavone phytoalexins and a pathogen-inducible flavone synthase II gene (SbFNSII) in sorghum | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong