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Article: Identification of flavone phytoalexins and a pathogen-inducible flavone synthase II gene (SbFNSII) in sorghum
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TitleIdentification of flavone phytoalexins and a pathogen-inducible flavone synthase II gene (SbFNSII) in sorghum
 
AuthorsDu, Y1
Chu, H1
Wang, M1
Chu, IK1
Lo, C1
 
KeywordsColletotrichum sublineolum
CYP93G3
Flavone synthase II
Flavones
Sorghum
 
Issue Date2010
 
PublisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/
 
CitationJournal Of Experimental Botany, 2010, v. 61 n. 4, p. 983-994 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jxb/erp364
 
AbstractFollowing inoculation with the anthracnose pathogen Colletotrichum sublineolum, seedlings of the sorghum resistant cultivar SC748-5 showed more rapid and elevated accumulation of luteolin than the susceptible cultivar BTx623. On the other hand, apigenin was the major flavone detected in infected BTx623 seedlings. Luteolin was demonstrated to show stronger inhibition of spore germination of C. sublineolum than apigenin. Because of their pathogen-inducible and antifungal nature, both flavone aglycones are considered sorghum phytoalexins. The key enzyme responsible for flavone biosynthesis has not been characterized in monocots. A sorghum pathogen-inducible gene encoding a cytochrome P450 protein (CYP93G3) in the uncharacterized CYP93G subfamily was identified. Transgenic expression of the P450 gene in Arabidopsis demonstrated that the encoded protein is a functional flavone synthase (FNS) II in planta. The sorghum gene was then termed SbFNSII. It is a single-copy gene located on chromosome 2 and the first FNSII gene characterized in a monocot. Metabolite analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in precursor ion scan mode revealed the accumulation of 2-hydroxynaringenin and 2-hydroxyeriodictyol hexosides in the transgenic Arabidopsis plants. Hence, SbFNSII appears to share a similar catalytic mechanism with the licorice and Medicago truncatula FNSIIs (CYP93B subfamily) by converting flavanones to flavone through the formation of 2-hydroxyflavanones.
 
ISSN0022-0957
2013 Impact Factor: 5.794
 
DOIhttp://dx.doi.org/10.1093/jxb/erp364
 
PubMed Central IDPMC2826645
 
ISI Accession Number IDWOS:000275567300005
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, ChinaHKU7527/06M
The University of Hong Kong Seed Funding200711159025
Funding Information:

This work was supported by the Research Grants Council of the Hong Kong Special Administrative Region, China (grant no. HKU7527/06M) and The University of Hong Kong Seed Funding program (project code 200711159025).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorDu, Y
 
dc.contributor.authorChu, H
 
dc.contributor.authorWang, M
 
dc.contributor.authorChu, IK
 
dc.contributor.authorLo, C
 
dc.date.accessioned2010-09-06T06:10:15Z
 
dc.date.available2010-09-06T06:10:15Z
 
dc.date.issued2010
 
dc.description.abstractFollowing inoculation with the anthracnose pathogen Colletotrichum sublineolum, seedlings of the sorghum resistant cultivar SC748-5 showed more rapid and elevated accumulation of luteolin than the susceptible cultivar BTx623. On the other hand, apigenin was the major flavone detected in infected BTx623 seedlings. Luteolin was demonstrated to show stronger inhibition of spore germination of C. sublineolum than apigenin. Because of their pathogen-inducible and antifungal nature, both flavone aglycones are considered sorghum phytoalexins. The key enzyme responsible for flavone biosynthesis has not been characterized in monocots. A sorghum pathogen-inducible gene encoding a cytochrome P450 protein (CYP93G3) in the uncharacterized CYP93G subfamily was identified. Transgenic expression of the P450 gene in Arabidopsis demonstrated that the encoded protein is a functional flavone synthase (FNS) II in planta. The sorghum gene was then termed SbFNSII. It is a single-copy gene located on chromosome 2 and the first FNSII gene characterized in a monocot. Metabolite analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in precursor ion scan mode revealed the accumulation of 2-hydroxynaringenin and 2-hydroxyeriodictyol hexosides in the transgenic Arabidopsis plants. Hence, SbFNSII appears to share a similar catalytic mechanism with the licorice and Medicago truncatula FNSIIs (CYP93B subfamily) by converting flavanones to flavone through the formation of 2-hydroxyflavanones.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationJournal Of Experimental Botany, 2010, v. 61 n. 4, p. 983-994 [How to Cite?]
DOI: http://dx.doi.org/10.1093/jxb/erp364
 
dc.identifier.doihttp://dx.doi.org/10.1093/jxb/erp364
 
dc.identifier.eissn1460-2431
 
dc.identifier.epage994
 
dc.identifier.hkuros169748
 
dc.identifier.isiWOS:000275567300005
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, ChinaHKU7527/06M
The University of Hong Kong Seed Funding200711159025
Funding Information:

This work was supported by the Research Grants Council of the Hong Kong Special Administrative Region, China (grant no. HKU7527/06M) and The University of Hong Kong Seed Funding program (project code 200711159025).

 
dc.identifier.issn0022-0957
2013 Impact Factor: 5.794
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC2826645
 
dc.identifier.pmid20007684
 
dc.identifier.scopuseid_2-s2.0-77649244513
 
dc.identifier.spage983
 
dc.identifier.urihttp://hdl.handle.net/10722/69068
 
dc.identifier.volume61
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Experimental Botany
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshCytochrome P-450 Enzyme System - chemistry - genetics - metabolism
 
dc.subject.meshFlavones - biosynthesis
 
dc.subject.meshPlant Proteins - chemistry - genetics - metabolism
 
dc.subject.meshSorghum - enzymology - genetics - metabolism - microbiology
 
dc.subject.meshTerpenes - metabolism
 
dc.subjectColletotrichum sublineolum
 
dc.subjectCYP93G3
 
dc.subjectFlavone synthase II
 
dc.subjectFlavones
 
dc.subjectSorghum
 
dc.titleIdentification of flavone phytoalexins and a pathogen-inducible flavone synthase II gene (SbFNSII) in sorghum
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong