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Article: Regulation of XAF1 expression in human colon cancer cell by interferon β: Activation by the transcription regulator STAT1
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TitleRegulation of XAF1 expression in human colon cancer cell by interferon β: Activation by the transcription regulator STAT1
 
AuthorsSun, Y2 1
Qiao, L1
Xia, HHX1
Lin, MCM1
Zou, B1
Yuan, Y2
Zhu, S1
Gu, Q1
Cheung, TK1
Kung, HF3
Yuen, MF1
Chan, AO1
Wong, BCY1
 
KeywordsColon cancer
IFNβ
Stat1
XAF1
 
Issue Date2008
 
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
 
CitationCancer Letters, 2008, v. 260 n. 1-2, p. 62-71 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.canlet.2007.10.014
 
AbstractXIAP-associated factor 1 (XAF1) is a novel tumor suppressor and interferon stimulated gene (ISG). Interferon β (IFNβ) exerts anti-proliferative effect and induces apoptosis through the Jak-Stat signaling cascade by the type I Interferon receptor (IFN-R), which initiates gene transcription of those biological effectors of IFNβ. The aim of this study is to determine the effect of IFNβ on XAF1 expression and the putative mechanisms mediated by the critical role of signal transducers and activators of transcription 1 (Stat1). Gene expression was detected by RT-PCR and Western blot analysis. The promoter activity of XAF1 was examined by luciferase reporter assay. The activity of interferon stimulated response element (ISRE) was assessed by electrophoretic mobility shift assay (EMSA) and quantitative chromatin immunoprecipitation assay (Q-ChIP). Results showed that IFNβ stimulated XAF1 promoter activity in colon cancer cell line DLD1 in a time- and dose-dependent manner. A high affinity ISRE binding element (ISRE-XAF1) was located in -55 to -66 nt upstream of the first ATG site of XAF1 gene. Deletion of ISRE-XAF1 completely abrogated basal and IFNβ-induced promoter activity. IFNβ-induced XAF1 expression was mediated by Stat1 through the interaction with ISRE-XAF1. Knocking down of the Stat1 expression and blocking its phosphorylation decreased IFNβ-induced XAF1 expression. Results suggested that induction of an immediate early response gene-XAF1 by IFNβ was mediated by the transcription regulator Stat1 through the ISRE site within the promoter region of XAF1 gene in colon cancer. © 2007 Elsevier Ireland Ltd. All rights reserved.
 
ISSN0304-3835
2013 Impact Factor: 5.016
 
DOIhttp://dx.doi.org/10.1016/j.canlet.2007.10.014
 
ISI Accession Number IDWOS:000253278400008
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorSun, Y
 
dc.contributor.authorQiao, L
 
dc.contributor.authorXia, HHX
 
dc.contributor.authorLin, MCM
 
dc.contributor.authorZou, B
 
dc.contributor.authorYuan, Y
 
dc.contributor.authorZhu, S
 
dc.contributor.authorGu, Q
 
dc.contributor.authorCheung, TK
 
dc.contributor.authorKung, HF
 
dc.contributor.authorYuen, MF
 
dc.contributor.authorChan, AO
 
dc.contributor.authorWong, BCY
 
dc.date.accessioned2010-09-06T06:10:10Z
 
dc.date.available2010-09-06T06:10:10Z
 
dc.date.issued2008
 
dc.description.abstractXIAP-associated factor 1 (XAF1) is a novel tumor suppressor and interferon stimulated gene (ISG). Interferon β (IFNβ) exerts anti-proliferative effect and induces apoptosis through the Jak-Stat signaling cascade by the type I Interferon receptor (IFN-R), which initiates gene transcription of those biological effectors of IFNβ. The aim of this study is to determine the effect of IFNβ on XAF1 expression and the putative mechanisms mediated by the critical role of signal transducers and activators of transcription 1 (Stat1). Gene expression was detected by RT-PCR and Western blot analysis. The promoter activity of XAF1 was examined by luciferase reporter assay. The activity of interferon stimulated response element (ISRE) was assessed by electrophoretic mobility shift assay (EMSA) and quantitative chromatin immunoprecipitation assay (Q-ChIP). Results showed that IFNβ stimulated XAF1 promoter activity in colon cancer cell line DLD1 in a time- and dose-dependent manner. A high affinity ISRE binding element (ISRE-XAF1) was located in -55 to -66 nt upstream of the first ATG site of XAF1 gene. Deletion of ISRE-XAF1 completely abrogated basal and IFNβ-induced promoter activity. IFNβ-induced XAF1 expression was mediated by Stat1 through the interaction with ISRE-XAF1. Knocking down of the Stat1 expression and blocking its phosphorylation decreased IFNβ-induced XAF1 expression. Results suggested that induction of an immediate early response gene-XAF1 by IFNβ was mediated by the transcription regulator Stat1 through the ISRE site within the promoter region of XAF1 gene in colon cancer. © 2007 Elsevier Ireland Ltd. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationCancer Letters, 2008, v. 260 n. 1-2, p. 62-71 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.canlet.2007.10.014
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.canlet.2007.10.014
 
dc.identifier.epage71
 
dc.identifier.hkuros139938
 
dc.identifier.isiWOS:000253278400008
 
dc.identifier.issn0304-3835
2013 Impact Factor: 5.016
 
dc.identifier.issue1-2
 
dc.identifier.openurl
 
dc.identifier.pmid18035482
 
dc.identifier.scopuseid_2-s2.0-38149058329
 
dc.identifier.spage62
 
dc.identifier.urihttp://hdl.handle.net/10722/69059
 
dc.identifier.volume260
 
dc.languageeng
 
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
 
dc.publisher.placeIreland
 
dc.relation.ispartofCancer Letters
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCancer Letters. Copyright © Elsevier Ireland Ltd.
 
dc.subject.meshBase Sequence
 
dc.subject.meshCell Line, Tumor
 
dc.subject.meshColonic Neoplasms - genetics - metabolism - pathology
 
dc.subject.meshDose-Response Relationship, Drug
 
dc.subject.meshGene Expression Regulation, Neoplastic
 
dc.subject.meshHumans
 
dc.subject.meshInterferon Regulatory Factor-1 - metabolism
 
dc.subject.meshInterferon-beta - metabolism - pharmacology
 
dc.subject.meshIntracellular Signaling Peptides and Proteins
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshNeoplasm Proteins - genetics - metabolism
 
dc.subject.meshPhosphorylation
 
dc.subject.meshPromoter Regions, Genetic
 
dc.subject.meshRNA Interference
 
dc.subject.meshRNA, Messenger - metabolism
 
dc.subject.meshRNA, Small Interfering - metabolism
 
dc.subject.meshRecombinant Proteins - metabolism
 
dc.subject.meshResponse Elements
 
dc.subject.meshSTAT1 Transcription Factor - metabolism
 
dc.subject.meshSignal Transduction - drug effects
 
dc.subject.meshTime Factors
 
dc.subject.meshTranscription, Genetic
 
dc.subject.meshTransfection
 
dc.subject.meshUp-Regulation
 
dc.subjectColon cancer
 
dc.subjectIFNβ
 
dc.subjectStat1
 
dc.subjectXAF1
 
dc.titleRegulation of XAF1 expression in human colon cancer cell by interferon β: Activation by the transcription regulator STAT1
 
dc.typeArticle
 
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<description.abstract>XIAP-associated factor 1 (XAF1) is a novel tumor suppressor and interferon stimulated gene (ISG). Interferon &#946; (IFN&#946;) exerts anti-proliferative effect and induces apoptosis through the Jak-Stat signaling cascade by the type I Interferon receptor (IFN-R), which initiates gene transcription of those biological effectors of IFN&#946;. The aim of this study is to determine the effect of IFN&#946; on XAF1 expression and the putative mechanisms mediated by the critical role of signal transducers and activators of transcription 1 (Stat1). Gene expression was detected by RT-PCR and Western blot analysis. The promoter activity of XAF1 was examined by luciferase reporter assay. The activity of interferon stimulated response element (ISRE) was assessed by electrophoretic mobility shift assay (EMSA) and quantitative chromatin immunoprecipitation assay (Q-ChIP). Results showed that IFN&#946; stimulated XAF1 promoter activity in colon cancer cell line DLD1 in a time- and dose-dependent manner. A high affinity ISRE binding element (ISRE-XAF1) was located in -55 to -66 nt upstream of the first ATG site of XAF1 gene. Deletion of ISRE-XAF1 completely abrogated basal and IFN&#946;-induced promoter activity. IFN&#946;-induced XAF1 expression was mediated by Stat1 through the interaction with ISRE-XAF1. Knocking down of the Stat1 expression and blocking its phosphorylation decreased IFN&#946;-induced XAF1 expression. Results suggested that induction of an immediate early response gene-XAF1 by IFN&#946; was mediated by the transcription regulator Stat1 through the ISRE site within the promoter region of XAF1 gene in colon cancer. &#169; 2007 Elsevier Ireland Ltd. All rights reserved.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. Ruijin Hospital
  3. Chinese University of Hong Kong