Article: Regulation of XAF1 expression in human colon cancer cell by interferon β: Activation by the transcription regulator STAT1

File Download Links for fulltext
(May Require Subscription)
Supplementary
  • Basic View
  • Metadata View
  • XML View
TitleRegulation of XAF1 expression in human colon cancer cell by interferon β: Activation by the transcription regulator STAT1
AuthorsSun, Y1 2
Qiao, L1
Xia, HHX1
Lin, MCM1
Zou, B1
Yuan, Y2
Zhu, S1
Gu, Q1
Cheung, TK1
Kung, HF3
Yuen, MF1
Chan, AO1
Wong, BCY1
KeywordsColon cancer
IFNβ
Stat1
XAF1
Issue Date2008
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
CitationCancer Letters, 2008, v. 260 n. 1-2, p. 62-71 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.canlet.2007.10.014
AbstractXIAP-associated factor 1 (XAF1) is a novel tumor suppressor and interferon stimulated gene (ISG). Interferon β (IFNβ) exerts anti-proliferative effect and induces apoptosis through the Jak-Stat signaling cascade by the type I Interferon receptor (IFN-R), which initiates gene transcription of those biological effectors of IFNβ. The aim of this study is to determine the effect of IFNβ on XAF1 expression and the putative mechanisms mediated by the critical role of signal transducers and activators of transcription 1 (Stat1). Gene expression was detected by RT-PCR and Western blot analysis. The promoter activity of XAF1 was examined by luciferase reporter assay. The activity of interferon stimulated response element (ISRE) was assessed by electrophoretic mobility shift assay (EMSA) and quantitative chromatin immunoprecipitation assay (Q-ChIP). Results showed that IFNβ stimulated XAF1 promoter activity in colon cancer cell line DLD1 in a time- and dose-dependent manner. A high affinity ISRE binding element (ISRE-XAF1) was located in -55 to -66 nt upstream of the first ATG site of XAF1 gene. Deletion of ISRE-XAF1 completely abrogated basal and IFNβ-induced promoter activity. IFNβ-induced XAF1 expression was mediated by Stat1 through the interaction with ISRE-XAF1. Knocking down of the Stat1 expression and blocking its phosphorylation decreased IFNβ-induced XAF1 expression. Results suggested that induction of an immediate early response gene-XAF1 by IFNβ was mediated by the transcription regulator Stat1 through the ISRE site within the promoter region of XAF1 gene in colon cancer. © 2007 Elsevier Ireland Ltd. All rights reserved.
ISSN0304-3835
2011 Impact Factor: 4.238
2011 SCImago Journal Rankings: 0.494
DOIhttp://dx.doi.org/10.1016/j.canlet.2007.10.014
ISI Accession Number IDWOS:000253278400008
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorSun, Y
dc.contributor.authorQiao, L
dc.contributor.authorXia, HHX
dc.contributor.authorLin, MCM
dc.contributor.authorZou, B
dc.contributor.authorYuan, Y
dc.contributor.authorZhu, S
dc.contributor.authorGu, Q
dc.contributor.authorCheung, TK
dc.contributor.authorKung, HF
dc.contributor.authorYuen, MF
dc.contributor.authorChan, AO
dc.contributor.authorWong, BCY
dc.date.accessioned2010-09-06T06:10:10Z
dc.date.available2010-09-06T06:10:10Z
dc.date.issued2008
dc.description.abstractXIAP-associated factor 1 (XAF1) is a novel tumor suppressor and interferon stimulated gene (ISG). Interferon β (IFNβ) exerts anti-proliferative effect and induces apoptosis through the Jak-Stat signaling cascade by the type I Interferon receptor (IFN-R), which initiates gene transcription of those biological effectors of IFNβ. The aim of this study is to determine the effect of IFNβ on XAF1 expression and the putative mechanisms mediated by the critical role of signal transducers and activators of transcription 1 (Stat1). Gene expression was detected by RT-PCR and Western blot analysis. The promoter activity of XAF1 was examined by luciferase reporter assay. The activity of interferon stimulated response element (ISRE) was assessed by electrophoretic mobility shift assay (EMSA) and quantitative chromatin immunoprecipitation assay (Q-ChIP). Results showed that IFNβ stimulated XAF1 promoter activity in colon cancer cell line DLD1 in a time- and dose-dependent manner. A high affinity ISRE binding element (ISRE-XAF1) was located in -55 to -66 nt upstream of the first ATG site of XAF1 gene. Deletion of ISRE-XAF1 completely abrogated basal and IFNβ-induced promoter activity. IFNβ-induced XAF1 expression was mediated by Stat1 through the interaction with ISRE-XAF1. Knocking down of the Stat1 expression and blocking its phosphorylation decreased IFNβ-induced XAF1 expression. Results suggested that induction of an immediate early response gene-XAF1 by IFNβ was mediated by the transcription regulator Stat1 through the ISRE site within the promoter region of XAF1 gene in colon cancer. © 2007 Elsevier Ireland Ltd. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCancer Letters, 2008, v. 260 n. 1-2, p. 62-71 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.canlet.2007.10.014
dc.identifier.doihttp://dx.doi.org/10.1016/j.canlet.2007.10.014
dc.identifier.epage71
dc.identifier.hkuros139938
dc.identifier.isiWOS:000253278400008
dc.identifier.issn0304-3835
2011 Impact Factor: 4.238
2011 SCImago Journal Rankings: 0.494
dc.identifier.issue1-2
dc.identifier.openurl
dc.identifier.pmid18035482
dc.identifier.scopuseid_2-s2.0-38149058329
dc.identifier.spage62
dc.identifier.urihttp://hdl.handle.net/10722/69059
dc.identifier.volume260
dc.languageeng
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
dc.publisher.placeIreland
dc.relation.ispartofCancer Letters
dc.relation.referencesReferences in Scopus
dc.rightsCancer Letters. Copyright © Elsevier Ireland Ltd.
dc.subject.meshBase Sequence
dc.subject.meshCell Line, Tumor
dc.subject.meshColonic Neoplasms - genetics - metabolism - pathology
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshGene Expression Regulation, Neoplastic
dc.subject.meshHumans
dc.subject.meshInterferon Regulatory Factor-1 - metabolism
dc.subject.meshInterferon-beta - metabolism - pharmacology
dc.subject.meshIntracellular Signaling Peptides and Proteins
dc.subject.meshMolecular Sequence Data
dc.subject.meshNeoplasm Proteins - genetics - metabolism
dc.subject.meshPhosphorylation
dc.subject.meshPromoter Regions, Genetic
dc.subject.meshRNA Interference
dc.subject.meshRNA, Messenger - metabolism
dc.subject.meshRNA, Small Interfering - metabolism
dc.subject.meshRecombinant Proteins - metabolism
dc.subject.meshResponse Elements
dc.subject.meshSTAT1 Transcription Factor - metabolism
dc.subject.meshSignal Transduction - drug effects
dc.subject.meshTime Factors
dc.subject.meshTranscription, Genetic
dc.subject.meshTransfection
dc.subject.meshUp-Regulation
dc.subjectColon cancer
dc.subjectIFNβ
dc.subjectStat1
dc.subjectXAF1
dc.titleRegulation of XAF1 expression in human colon cancer cell by interferon β: Activation by the transcription regulator STAT1
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Ruijin Hospital
  3. Chinese University of Hong Kong