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Article: Two-dimensional differential gel electrophoresis/analysis of diethylnitrosamine induced rat hepatocellular carcinoma

TitleTwo-dimensional differential gel electrophoresis/analysis of diethylnitrosamine induced rat hepatocellular carcinoma
Authors
KeywordsDiethylnitrosamine
Glutathione S-transferase
Hepatocellular carcinoma
Two-dimensional differential gel electrophoresis
Issue Date2008
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 2008, v. 122 n. 12, p. 2682-2688 How to Cite?
AbstractDiethylnitrosamine (DEN) is a known carcinogen that can alkylate DNA molecules. In rats, DEN-induced hepatocellular carcinoma (HCC) model is well established. In this study, we used a two-dimensional differential gel electrophoresis (2D-DIGE) system and liquid chromatography/mass spectrometry/mass spectrometry to identify the differential expression protein profiles between the DEN-induced HCC and healthy liver cells. Western blotting and semiquantitative RT-PCR were used to further confirm the results. Seventeen differentially expressed spots were identified in DEN-induced HCC cells. Among all, the most prominent upregulated proteins include the members of the glutathione S-transferase super family, aldo-keto reductase superfamily and proteins involved in the response to oxidative stress. Downregulation was observed in 2 proteins that were known to contribute to hepatic dysfunction. This study provides the first comprehensive protein profiling of the DEN-induced HCC in rats. This model simulates the differential protein expression of human HCC and may be useful for further understanding the mechanism of HCC tumorigenesis. © 2008 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/68996
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQi, Yen_HK
dc.contributor.authorChen, Xen_HK
dc.contributor.authorChan, CYen_HK
dc.contributor.authorLi, Den_HK
dc.contributor.authorYuan, Cen_HK
dc.contributor.authorYu, Fen_HK
dc.contributor.authorLin, MCen_HK
dc.contributor.authorYew, DTen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorLai, Len_HK
dc.date.accessioned2010-09-06T06:09:36Z-
dc.date.available2010-09-06T06:09:36Z-
dc.date.issued2008en_HK
dc.identifier.citationInternational Journal Of Cancer, 2008, v. 122 n. 12, p. 2682-2688en_HK
dc.identifier.issn0020-7136en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68996-
dc.description.abstractDiethylnitrosamine (DEN) is a known carcinogen that can alkylate DNA molecules. In rats, DEN-induced hepatocellular carcinoma (HCC) model is well established. In this study, we used a two-dimensional differential gel electrophoresis (2D-DIGE) system and liquid chromatography/mass spectrometry/mass spectrometry to identify the differential expression protein profiles between the DEN-induced HCC and healthy liver cells. Western blotting and semiquantitative RT-PCR were used to further confirm the results. Seventeen differentially expressed spots were identified in DEN-induced HCC cells. Among all, the most prominent upregulated proteins include the members of the glutathione S-transferase super family, aldo-keto reductase superfamily and proteins involved in the response to oxidative stress. Downregulation was observed in 2 proteins that were known to contribute to hepatic dysfunction. This study provides the first comprehensive protein profiling of the DEN-induced HCC in rats. This model simulates the differential protein expression of human HCC and may be useful for further understanding the mechanism of HCC tumorigenesis. © 2008 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_HK
dc.relation.ispartofInternational Journal of Canceren_HK
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectDiethylnitrosamineen_HK
dc.subjectGlutathione S-transferaseen_HK
dc.subjectHepatocellular carcinomaen_HK
dc.subjectTwo-dimensional differential gel electrophoresisen_HK
dc.titleTwo-dimensional differential gel electrophoresis/analysis of diethylnitrosamine induced rat hepatocellular carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=122&issue=12&spage=2682&epage=8&date=2008&atitle=Two-dimensional+Differential+Gel+Electrophoresis/analysis+of+Diethylnitrosamine+Induced+Rat+Hepatocellular+Carcinomaen_HK
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/ijc.23464en_HK
dc.identifier.pmid18351647-
dc.identifier.scopuseid_2-s2.0-43049128176en_HK
dc.identifier.hkuros141165en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-43049128176&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume122en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2682en_HK
dc.identifier.epage2688en_HK
dc.identifier.isiWOS:000256713800005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridQi, Y=24171887800en_HK
dc.identifier.scopusauthoridChen, X=24170717900en_HK
dc.identifier.scopusauthoridChan, CY=22033276600en_HK
dc.identifier.scopusauthoridLi, D=26324923700en_HK
dc.identifier.scopusauthoridYuan, C=8879550700en_HK
dc.identifier.scopusauthoridYu, F=55207919900en_HK
dc.identifier.scopusauthoridLin, MC=7404816359en_HK
dc.identifier.scopusauthoridYew, DT=7007034694en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridLai, L=12445800200en_HK

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