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Article: Native fluorescence detection of flavin derivatives by microchip capillary electrophoresis with laser-induced fluorescence intensified charge-coupled device detection

TitleNative fluorescence detection of flavin derivatives by microchip capillary electrophoresis with laser-induced fluorescence intensified charge-coupled device detection
Authors
KeywordsChip technology
Detection, electrophoresis
Flavins
Laser-induced fluorescence detection
Microfluidics
Riboflavin
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2004, v. 1027 n. 1-2, p. 223-229 How to Cite?
AbstractTo widen the scope of laser-induced fluorescence (LIF) for detection in microchip capillary electrophoresis (CE), a microchip CE LIF-ICCD (intensified charge-coupled device) system based on a tunable wavelength dye laser pumped by a pico-second pulse nitrogen laser for excitation and a spectrograph with ICCD for detection had developed to demonstrate the enhancement in detection sensitivity by the following three approaches: direct detection of native fluorescence, improvement of signal-to-noise ratio by pulse laser excitation and time delay detection, and selective spectral acquisition by multi-channel detection. Riboflavin, flavin mononucleotide (FMN) and flavin-adenine dinucleotide (FAD) have been selected as they are dietetically important and microchip CE provides a promising onsite detection method. The results indicate a strong effect of wavelength on detection sensitivity and the need to tune wavelength for direct detection. Under optimized conditions (excitation 450nm, emission 520nm, gate delay time 45ns, 20mM phosphate buffer at pH 7.1), the following results were obtained under static condition: Working ranges (0.6-350μg/l, r>0.99), detection limits (0.15-1.0μg/l) and peak height repeatability (1.8-2.2% R.S.D.), all within the applicability range for body fluids or beverages such as human urine and cow milk. Baseline separation of three flavins was obtained under dynamic condition and the fluorescence spectra acquired assist the identification of alkaline-degraded products of riboflavin. Thus, the capability to check peak purity and identify unknown peaks has been demonstrated. © 2003 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68985
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQin, Jen_HK
dc.contributor.authorFung, Yen_HK
dc.contributor.authorZhu, Den_HK
dc.contributor.authorLin, Ben_HK
dc.date.accessioned2010-09-06T06:09:30Z-
dc.date.available2010-09-06T06:09:30Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Chromatography A, 2004, v. 1027 n. 1-2, p. 223-229en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68985-
dc.description.abstractTo widen the scope of laser-induced fluorescence (LIF) for detection in microchip capillary electrophoresis (CE), a microchip CE LIF-ICCD (intensified charge-coupled device) system based on a tunable wavelength dye laser pumped by a pico-second pulse nitrogen laser for excitation and a spectrograph with ICCD for detection had developed to demonstrate the enhancement in detection sensitivity by the following three approaches: direct detection of native fluorescence, improvement of signal-to-noise ratio by pulse laser excitation and time delay detection, and selective spectral acquisition by multi-channel detection. Riboflavin, flavin mononucleotide (FMN) and flavin-adenine dinucleotide (FAD) have been selected as they are dietetically important and microchip CE provides a promising onsite detection method. The results indicate a strong effect of wavelength on detection sensitivity and the need to tune wavelength for direct detection. Under optimized conditions (excitation 450nm, emission 520nm, gate delay time 45ns, 20mM phosphate buffer at pH 7.1), the following results were obtained under static condition: Working ranges (0.6-350μg/l, r>0.99), detection limits (0.15-1.0μg/l) and peak height repeatability (1.8-2.2% R.S.D.), all within the applicability range for body fluids or beverages such as human urine and cow milk. Baseline separation of three flavins was obtained under dynamic condition and the fluorescence spectra acquired assist the identification of alkaline-degraded products of riboflavin. Thus, the capability to check peak purity and identify unknown peaks has been demonstrated. © 2003 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectChip technologyen_HK
dc.subjectDetection, electrophoresisen_HK
dc.subjectFlavinsen_HK
dc.subjectLaser-induced fluorescence detectionen_HK
dc.subjectMicrofluidicsen_HK
dc.subjectRiboflavinen_HK
dc.titleNative fluorescence detection of flavin derivatives by microchip capillary electrophoresis with laser-induced fluorescence intensified charge-coupled device detectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1027&spage=223&epage=229&date=2004&atitle=Native+fluorescence+detection+of+flavin+derivatives+by+microchip+capillary+electrophoresis+with+laser-induced+fluorescence+intensified+charge-coupled+device+detection+en_HK
dc.identifier.emailFung, Y:ysfung@hku.hken_HK
dc.identifier.authorityFung, Y=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chroma.2003.10.055en_HK
dc.identifier.pmid14971506-
dc.identifier.scopuseid_2-s2.0-0346023067en_HK
dc.identifier.hkuros93166en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0346023067&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1027en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage223en_HK
dc.identifier.epage229en_HK
dc.identifier.isiWOS:000188498900031-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridQin, J=7402895572en_HK
dc.identifier.scopusauthoridFung, Y=13309754700en_HK
dc.identifier.scopusauthoridZhu, D=7403599128en_HK
dc.identifier.scopusauthoridLin, B=7403508047en_HK

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