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Article: Immunosensor for the differentiation and detection of Salmonella species based on a quartz crystal microbalance

TitleImmunosensor for the differentiation and detection of Salmonella species based on a quartz crystal microbalance
Authors
KeywordsImmunosensors
Microbalance
Monoclonal antibodies
Polyethyleneimine
Salmonella
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bios
Citation
Biosensors And Bioelectronics, 2002, v. 17 n. 8, p. 676-684 How to Cite?
AbstractImmunosensors based on the microgravimetric quartz crystal microbalance (QCM) technique have been developed for the detection of Salmonella species from serogroups A, B and D. Salmonella serogroup-specific murine monoclonal antibodies, respectively, raised against these serogroups were immobilized onto the silver electrodes of piezoelectric (PZ) crystals by cross-linkage via glutaraldehyde (GA) to the electrode surfaces pre-coated with thin polyethyleneimine (PEI) layer. The specific immunosensors developed gave responses in linear ranges from 10 5 to 5×10 8 cells per ml with no significant interference from other strains of Salmonella and Escherichia coli up to 10 8 cells per ml. They showed good repeatability and excellent linear range, achieving detection limits down to 10 4 cells per ml with ability to distinguish different strains of Salmonella. These biosensors exhibited an exquisite specificity evidenced by their ability to discriminate antigens, the structures of which differ only by the isomeric form of di-deoxyhexose. The antibody-modified crystals showed no loss in activity over 4 days under storage at 4°C. © 2002 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68981
ISSN
2015 Impact Factor: 7.476
2015 SCImago Journal Rankings: 2.150
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, YYen_HK
dc.contributor.authorNg, SPen_HK
dc.contributor.authorNg, MHen_HK
dc.contributor.authorSi, SHen_HK
dc.contributor.authorYao, SZen_HK
dc.contributor.authorFung, YSen_HK
dc.date.accessioned2010-09-06T06:09:28Z-
dc.date.available2010-09-06T06:09:28Z-
dc.date.issued2002en_HK
dc.identifier.citationBiosensors And Bioelectronics, 2002, v. 17 n. 8, p. 676-684en_HK
dc.identifier.issn0956-5663en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68981-
dc.description.abstractImmunosensors based on the microgravimetric quartz crystal microbalance (QCM) technique have been developed for the detection of Salmonella species from serogroups A, B and D. Salmonella serogroup-specific murine monoclonal antibodies, respectively, raised against these serogroups were immobilized onto the silver electrodes of piezoelectric (PZ) crystals by cross-linkage via glutaraldehyde (GA) to the electrode surfaces pre-coated with thin polyethyleneimine (PEI) layer. The specific immunosensors developed gave responses in linear ranges from 10 5 to 5×10 8 cells per ml with no significant interference from other strains of Salmonella and Escherichia coli up to 10 8 cells per ml. They showed good repeatability and excellent linear range, achieving detection limits down to 10 4 cells per ml with ability to distinguish different strains of Salmonella. These biosensors exhibited an exquisite specificity evidenced by their ability to discriminate antigens, the structures of which differ only by the isomeric form of di-deoxyhexose. The antibody-modified crystals showed no loss in activity over 4 days under storage at 4°C. © 2002 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biosen_HK
dc.relation.ispartofBiosensors and Bioelectronicsen_HK
dc.rightsBiosensors and Bioelectronics. Copyright © Elsevier BV.en_HK
dc.subjectImmunosensorsen_HK
dc.subjectMicrobalanceen_HK
dc.subjectMonoclonal antibodiesen_HK
dc.subjectPolyethyleneimineen_HK
dc.subjectSalmonellaen_HK
dc.titleImmunosensor for the differentiation and detection of Salmonella species based on a quartz crystal microbalanceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0956-5663&volume=17&spage=676&epage=684&date=2002&atitle=Immunosensor+for+the+differentiation+and+detection+of+Salmonella+species+based+on+a+quartz+crystal+microbalanceen_HK
dc.identifier.emailFung, YS:ysfung@hku.hken_HK
dc.identifier.authorityFung, YS=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0956-5663(02)00030-1en_HK
dc.identifier.pmid12052353-
dc.identifier.scopuseid_2-s2.0-0036015962en_HK
dc.identifier.hkuros68494en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036015962&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue8en_HK
dc.identifier.spage676en_HK
dc.identifier.epage684en_HK
dc.identifier.isiWOS:000176900000006-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWong, YY=7403041805en_HK
dc.identifier.scopusauthoridNg, SP=8862966400en_HK
dc.identifier.scopusauthoridNg, MH=7202076421en_HK
dc.identifier.scopusauthoridSi, SH=7006049232en_HK
dc.identifier.scopusauthoridYao, SZ=35619833600en_HK
dc.identifier.scopusauthoridFung, YS=13309754700en_HK

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