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Article: Electrophoretic migration behavior of DNA fragments in polymer solution

TitleElectrophoretic migration behavior of DNA fragments in polymer solution
Authors
KeywordsCapillary electrophoresis
DNA separation
Entagled polymer solution theory
Polymer coil shrinking theory
Issue Date2001
PublisherWiley - V C H Verlag GmbH & Co KGaA.
Citation
Electrophoresis, 2001, v. 22 n. 11, p. 2150-2158 How to Cite?
AbstractA newly developed polymer coil shrinking theory is described and compared with the existing entangled solution theory to explain electrophoretic migration behaviour of DNA in hydroxypropylmethylcellulose (HPMC) polymer solution in buffer containing 100 mM tris(hydroxymethyl)aminomethane 100 mM boric acid, 2 mM ethylenediamine-tetraacetic acid at pH 8.3. The polymer coil shrinking theory gave a better model to explain the results obtained. The polymer coil shrinking concentration, C s, was found to be 0.305% and the uniform entangled concentration, C +, 0.806%. The existence of three regions (the dilute, semidilute, and concentrated solution) at different polymer concentrations enables a better understanding of the system to guide the selection of the best conditions to separate DNA fragments. For separating large fragments (700/800 bp), dilute solutions (HPMC < 0.3%) should be used to achieve a short migration time (10 min). For small fragments (200/300 bp), concentrated solutions are preferred to obtain constant resolution and uniform separation. The best resolution is 0.6% HPMC due to a combined interaction of the polymer coils and the entangled structure. The possibility of DNA separation in semidilute solution is often neglected and the present results indicate that this region has a promising potential for analytical separation of DNA fragments.
Persistent Identifierhttp://hdl.handle.net/10722/68978
ISSN
2015 Impact Factor: 2.482
2015 SCImago Journal Rankings: 0.896
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, Yen_HK
dc.contributor.authorLin, Ben_HK
dc.contributor.authorFung, YSen_HK
dc.date.accessioned2010-09-06T06:09:26Z-
dc.date.available2010-09-06T06:09:26Z-
dc.date.issued2001en_HK
dc.identifier.citationElectrophoresis, 2001, v. 22 n. 11, p. 2150-2158en_HK
dc.identifier.issn0173-0835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68978-
dc.description.abstractA newly developed polymer coil shrinking theory is described and compared with the existing entangled solution theory to explain electrophoretic migration behaviour of DNA in hydroxypropylmethylcellulose (HPMC) polymer solution in buffer containing 100 mM tris(hydroxymethyl)aminomethane 100 mM boric acid, 2 mM ethylenediamine-tetraacetic acid at pH 8.3. The polymer coil shrinking theory gave a better model to explain the results obtained. The polymer coil shrinking concentration, C s, was found to be 0.305% and the uniform entangled concentration, C +, 0.806%. The existence of three regions (the dilute, semidilute, and concentrated solution) at different polymer concentrations enables a better understanding of the system to guide the selection of the best conditions to separate DNA fragments. For separating large fragments (700/800 bp), dilute solutions (HPMC < 0.3%) should be used to achieve a short migration time (10 min). For small fragments (200/300 bp), concentrated solutions are preferred to obtain constant resolution and uniform separation. The best resolution is 0.6% HPMC due to a combined interaction of the polymer coils and the entangled structure. The possibility of DNA separation in semidilute solution is often neglected and the present results indicate that this region has a promising potential for analytical separation of DNA fragments.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA.en_HK
dc.relation.ispartofElectrophoresisen_HK
dc.subjectCapillary electrophoresisen_HK
dc.subjectDNA separationen_HK
dc.subjectEntagled polymer solution theoryen_HK
dc.subjectPolymer coil shrinking theoryen_HK
dc.titleElectrophoretic migration behavior of DNA fragments in polymer solutionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0173-0835&volume=22&spage=2150&epage=2158&date=2001&atitle=Electrophoretic+migration+behavior+of+DNA+fragments+in+polymer+solutionen_HK
dc.identifier.emailFung, YS:ysfung@hku.hken_HK
dc.identifier.authorityFung, YS=rp00697en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/1522-2683(20017)22:11<2150::AID-ELPS2150>3.0.CO;2-Uen_HK
dc.identifier.pmid11504046-
dc.identifier.scopuseid_2-s2.0-0034927031en_HK
dc.identifier.hkuros68485en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034927031&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue11en_HK
dc.identifier.spage2150en_HK
dc.identifier.epage2158en_HK
dc.identifier.isiWOS:000170175800005-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridJin, Y=39861604500en_HK
dc.identifier.scopusauthoridLin, B=7403508047en_HK
dc.identifier.scopusauthoridFung, YS=13309754700en_HK

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